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Article: Gold (III) porphyrin complexes induce apoptosis and cell cycle arrest and inhibit tumor growth in colon cancer

TitleGold (III) porphyrin complexes induce apoptosis and cell cycle arrest and inhibit tumor growth in colon cancer
Authors
KeywordsApoptosis
Cell cycle arrest
Colon cancer
Complexes
Gold (III)
Issue Date2009
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 2009, v. 115 n. 19, p. 4459-4469 How to Cite?
AbstractBACKGROUND: Gold (III) compounds have exhibited favorable antitumor properties both in vitro and in vivo. In a previous study, the authors reported that the novel gold (III) complex 1a (gold 1a) exhibited strong cytotoxicity in some tumor cell lines. In the current study, the effect of gold 1a was investigated on colon cancer cells. METHODS: The cytotoxicity of gold 1a was determined by using the 3-(4,5-dimethyl-2-thihazyl)-2,5-diphenyl-2H-tetrazolium bromide method. Flow cytometry was used to detect apoptosis and cell cycle. The expression of protein was evaluated by Western blot assay. Tumor growth in vivo was evaluated in nude mice. RESULTS: Gold 1a exhibited marked cytotoxic effects in vitro to human colon cancer, and the concentration of drug required to inhibit cell growth by 50% compared with control (IC50) values ranged from 0.2 lM to 3.4 lM, which represented 8.7-fold to 20.8-fold greater potency than that of cisplatin. Gold 1a significantly induced apoptosis and cell cycle arrest and cleaved caspase 3, caspase 7, and poly( ADP-ribose) polymerase; released cytochrome C, and up-regulated p53, p21, p27, and Bax. In vivo, intraperitoneal injection of gold 1a at doses of 1.5 mg/kg and 3.0 mg/kg significantly inhibited tumor cell proliferation, induced apoptosis, and suppressed colon cancer tumor growth. An acute toxicology study indicated that gold 1a at effective antitumor concentrations did not cause any toxic side effects in mice. CONCLUSIONS: The current results suggested that gold 1a may be a new potential therapeutic drug for colon cancer. © 2009 American Cancer Society.
Persistent Identifierhttp://hdl.handle.net/10722/68974
ISSN
2015 Impact Factor: 5.649
2015 SCImago Journal Rankings: 3.188
ISI Accession Number ID
Funding AgencyGrant Number
Areas of Excellence SchemeAoE/ P-10/01
Gastroenterological Research Fund of University of Hong Kong, Hong Kong, China
Funding Information:

Supported by grants from the Areas of Excellence Scheme (AoE/ P-10/01) administered by the University Grants Council (Hong Kong SAR, China) and the Gastroenterological Research Fund of University of Hong Kong, Hong Kong, China.

References

 

DC FieldValueLanguage
dc.contributor.authorTu, Sen_HK
dc.contributor.authorSun, RWYen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorJian, TCen_HK
dc.contributor.authorZou, Ben_HK
dc.contributor.authorGu, Qen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorChe, CMen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-06T06:09:24Z-
dc.date.available2010-09-06T06:09:24Z-
dc.date.issued2009en_HK
dc.identifier.citationCancer, 2009, v. 115 n. 19, p. 4459-4469en_HK
dc.identifier.issn0008-543Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68974-
dc.description.abstractBACKGROUND: Gold (III) compounds have exhibited favorable antitumor properties both in vitro and in vivo. In a previous study, the authors reported that the novel gold (III) complex 1a (gold 1a) exhibited strong cytotoxicity in some tumor cell lines. In the current study, the effect of gold 1a was investigated on colon cancer cells. METHODS: The cytotoxicity of gold 1a was determined by using the 3-(4,5-dimethyl-2-thihazyl)-2,5-diphenyl-2H-tetrazolium bromide method. Flow cytometry was used to detect apoptosis and cell cycle. The expression of protein was evaluated by Western blot assay. Tumor growth in vivo was evaluated in nude mice. RESULTS: Gold 1a exhibited marked cytotoxic effects in vitro to human colon cancer, and the concentration of drug required to inhibit cell growth by 50% compared with control (IC50) values ranged from 0.2 lM to 3.4 lM, which represented 8.7-fold to 20.8-fold greater potency than that of cisplatin. Gold 1a significantly induced apoptosis and cell cycle arrest and cleaved caspase 3, caspase 7, and poly( ADP-ribose) polymerase; released cytochrome C, and up-regulated p53, p21, p27, and Bax. In vivo, intraperitoneal injection of gold 1a at doses of 1.5 mg/kg and 3.0 mg/kg significantly inhibited tumor cell proliferation, induced apoptosis, and suppressed colon cancer tumor growth. An acute toxicology study indicated that gold 1a at effective antitumor concentrations did not cause any toxic side effects in mice. CONCLUSIONS: The current results suggested that gold 1a may be a new potential therapeutic drug for colon cancer. © 2009 American Cancer Society.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_HK
dc.relation.ispartofCanceren_HK
dc.rightsCancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectApoptosisen_HK
dc.subjectCell cycle arresten_HK
dc.subjectColon canceren_HK
dc.subjectComplexesen_HK
dc.subjectGold (III)en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntineoplastic Agents - therapeutic useen_HK
dc.subject.meshApoptosis - drug effectsen_HK
dc.subject.meshCell Cycle - drug effectsen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshColonic Neoplasms - drug therapyen_HK
dc.subject.meshDrug Screening Assays, Antitumoren_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMetalloporphyrins - adverse effects - pharmacology - therapeutic useen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshXenograft Model Antitumor Assaysen_HK
dc.titleGold (III) porphyrin complexes induce apoptosis and cell cycle arrest and inhibit tumor growth in colon canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-543X&volume=115&issue=19&spage=4459&epage=4469&date=2009&atitle=Gold+(III)+Porphyrin+Complexes+Induce+Apoptosis+and+Cell+Cycle+Arrest+and+Inhibit+Tumor+Growth+in+Colon+Canceren_HK
dc.identifier.emailSun, RWY:rwysun@hku.hken_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.emailChe, CM:cmche@hku.hken_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.authoritySun, RWY=rp00781en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/cncr.24514en_HK
dc.identifier.pmid19572413-
dc.identifier.scopuseid_2-s2.0-70349268321en_HK
dc.identifier.hkuros167195en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349268321&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume115en_HK
dc.identifier.issue19en_HK
dc.identifier.spage4459en_HK
dc.identifier.epage4469en_HK
dc.identifier.isiWOS:000270375700007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTu, S=7202726555en_HK
dc.identifier.scopusauthoridSun, RWY=26325835800en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridJian, TC=26867735900en_HK
dc.identifier.scopusauthoridZou, B=35228257300en_HK
dc.identifier.scopusauthoridGu, Q=24469982400en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK

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