File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: DNA binding and cytotoxicity of ruthenium(II) and rhenium(I) complexes of 2-amino-4-phenylamino-6-(2-pyridyl)-1,3,5-triazine

TitleDNA binding and cytotoxicity of ruthenium(II) and rhenium(I) complexes of 2-amino-4-phenylamino-6-(2-pyridyl)-1,3,5-triazine
Authors
Issue Date2007
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ic
Citation
Inorganic Chemistry, 2007, v. 46 n. 3, p. 740-749 How to Cite?
Abstract[Ru(Bu2bpy)2(2-appt)](PF6)2 [1·(PF6)2, tBu2bpy = 4,4′-di-tert-butyl-2,2′-bipyridine, 2-appt = 2-amino-4-phenylamino- 6-(2-pyridyl)-1,3,5-triazine] and [Re(CO)3(2-appt)Cl] (2) were prepared and characterized by X-ray crystal analysis. The binding of 1·(PF6)2 and 2 to calf thymus DNA (ct DNA) led to increases in the DNA melting temperature (ΔTm = +12°C), modest hypochromism (29% and 5% of the absorption bands at λ max = 450 and 376 nm, respectively), and insignificant shifts in the absorption maxima. The binding constants of 1·(PF6) 2 and 2 with ct DNA, as determined by absorption titration, are (8.9 ± 0.5) × 104 and (3.6 ± 0.1) × 10 4 dm3 mol-1, respectively. UV-vis absorption titration, DNA melting studies, and competition dialysis using synthetic oligonucleotides [poly(dA-dT)2 and poly(dG-dC)2] revealed that 1·(PF6)2 and 2 exhibit a binding preference for AT sequences. A modeling study on the interaction between 1 or 2 and B-DNA revealed that the minor groove is the most favored binding site and an extensive hydrogen-bonding network is formed. As determined by MTT assays, 1·(PF6)2 and 2 exhibited moderate cytotoxicities toward several human cancer cell lines (KB-3-1, HepG2, and HeLa), as well as a multi-drug-resistant cancer cell line (KB-V-1). According to confocal microscopic and flow cytometric studies, 1·(PF6)2 and 2 induced apoptosis (50-60%) in cancer cells with <5% necrosis detected. © 2007 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/68956
ISSN
2015 Impact Factor: 4.82
2015 SCImago Journal Rankings: 1.873
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, DLen_HK
dc.contributor.authorChe, CMen_HK
dc.contributor.authorSiu, FMen_HK
dc.contributor.authorYang, Men_HK
dc.contributor.authorWong, KYen_HK
dc.date.accessioned2010-09-06T06:09:15Z-
dc.date.available2010-09-06T06:09:15Z-
dc.date.issued2007en_HK
dc.identifier.citationInorganic Chemistry, 2007, v. 46 n. 3, p. 740-749en_HK
dc.identifier.issn0020-1669en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68956-
dc.description.abstract[Ru(Bu2bpy)2(2-appt)](PF6)2 [1·(PF6)2, tBu2bpy = 4,4′-di-tert-butyl-2,2′-bipyridine, 2-appt = 2-amino-4-phenylamino- 6-(2-pyridyl)-1,3,5-triazine] and [Re(CO)3(2-appt)Cl] (2) were prepared and characterized by X-ray crystal analysis. The binding of 1·(PF6)2 and 2 to calf thymus DNA (ct DNA) led to increases in the DNA melting temperature (ΔTm = +12°C), modest hypochromism (29% and 5% of the absorption bands at λ max = 450 and 376 nm, respectively), and insignificant shifts in the absorption maxima. The binding constants of 1·(PF6) 2 and 2 with ct DNA, as determined by absorption titration, are (8.9 ± 0.5) × 104 and (3.6 ± 0.1) × 10 4 dm3 mol-1, respectively. UV-vis absorption titration, DNA melting studies, and competition dialysis using synthetic oligonucleotides [poly(dA-dT)2 and poly(dG-dC)2] revealed that 1·(PF6)2 and 2 exhibit a binding preference for AT sequences. A modeling study on the interaction between 1 or 2 and B-DNA revealed that the minor groove is the most favored binding site and an extensive hydrogen-bonding network is formed. As determined by MTT assays, 1·(PF6)2 and 2 exhibited moderate cytotoxicities toward several human cancer cell lines (KB-3-1, HepG2, and HeLa), as well as a multi-drug-resistant cancer cell line (KB-V-1). According to confocal microscopic and flow cytometric studies, 1·(PF6)2 and 2 induced apoptosis (50-60%) in cancer cells with <5% necrosis detected. © 2007 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/icen_HK
dc.relation.ispartofInorganic Chemistryen_HK
dc.titleDNA binding and cytotoxicity of ruthenium(II) and rhenium(I) complexes of 2-amino-4-phenylamino-6-(2-pyridyl)-1,3,5-triazineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-1669&volume=46&spage=740&epage=749&date=2007&atitle=DNA+Binding+and+Cytotoxicity+of+Ruthenium(II)+and+Rhenium(I)+Complexes+of+2-Amino-4-Phenylamino-6-(2-Pyridyl)-1,3,5-Triazineen_HK
dc.identifier.emailMa, DL:edmondma@hku.hken_HK
dc.identifier.emailChe, CM:cmche@hku.hken_HK
dc.identifier.emailSiu, FM:fmsiu@hku.hken_HK
dc.identifier.authorityMa, DL=rp00760en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.identifier.authoritySiu, FM=rp00776en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/ic061518sen_HK
dc.identifier.pmid17257015-
dc.identifier.scopuseid_2-s2.0-33847096353en_HK
dc.identifier.hkuros128148en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33847096353&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue3en_HK
dc.identifier.spage740en_HK
dc.identifier.epage749en_HK
dc.identifier.isiWOS:000243789400018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMa, DL=7402075538en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.scopusauthoridSiu, FM=6701518489en_HK
dc.identifier.scopusauthoridYang, M=7404925734en_HK
dc.identifier.scopusauthoridWong, KY=7404760030en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats