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Article: Genotyping the -6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis

TitleGenotyping the -6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis
Authors
KeywordsAngiotensinogen gene
Genotyping
Microchip electrophoresis
Miniaturization
Polymorphism analysis
Issue Date2005
PublisherWiley - V C H Verlag GmbH & Co KGaA.
Citation
Electrophoresis, 2005, v. 26 n. 1, p. 219-224 How to Cite?
AbstractAngiotensinogen (AGT) gene has been regarded as one of the candidate genes for essential hypertension. In our study, the role of AGT gene as a putatively predisposing gene for hypertension was evaluated by genotyping a A (-6) G polymorphism in the core promoter region in 123 patients with essential hypertension and 103 healthy controls. A microchip electrophoresis method coupled with polymorphism chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay was used for genotyping the A (-6) G single-nucleotide polymorphism. The separation and detection of the digested PCR amplicons were completed just in 280 s or less. The genotype frequency fulfilled the criteria of the Hardy-Weinbery equilibrium (X2 = 3.067, P > 0.05). The results showed a higher frequency of the -6 A allele (0.70) in the normotensive subjects, which is higher than those reported in Germany (0.47) and Czech (0.40) populations, but similar to that found in Japanese populations (0.73). The frequencies of genotype AA, AG, and GG were 0.46, 0.49, and 0.05 in hypertensive subjects, and 0.44, 0.53, and 0.03 in control subjects. There is no significant difference in the distributions of the genotype and allele between the two groups (X2 = 0.88, P > 0.05; X2 = 0.024, P > 0.05). These findings differ from some of the results obtained in other ethnic groups, indicating the potential importance of ethnic origin in the assessment of genetic risk identifiers for a complex disease. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/68909
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQin, Jen_HK
dc.contributor.authorLiu, Zen_HK
dc.contributor.authorWu, Den_HK
dc.contributor.authorZhu, Nen_HK
dc.contributor.authorZhou, Xen_HK
dc.contributor.authorFung, Yen_HK
dc.contributor.authorLin, Ben_HK
dc.date.accessioned2010-09-06T06:08:49Z-
dc.date.available2010-09-06T06:08:49Z-
dc.date.issued2005en_HK
dc.identifier.citationElectrophoresis, 2005, v. 26 n. 1, p. 219-224en_HK
dc.identifier.issn0173-0835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68909-
dc.description.abstractAngiotensinogen (AGT) gene has been regarded as one of the candidate genes for essential hypertension. In our study, the role of AGT gene as a putatively predisposing gene for hypertension was evaluated by genotyping a A (-6) G polymorphism in the core promoter region in 123 patients with essential hypertension and 103 healthy controls. A microchip electrophoresis method coupled with polymorphism chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay was used for genotyping the A (-6) G single-nucleotide polymorphism. The separation and detection of the digested PCR amplicons were completed just in 280 s or less. The genotype frequency fulfilled the criteria of the Hardy-Weinbery equilibrium (X2 = 3.067, P > 0.05). The results showed a higher frequency of the -6 A allele (0.70) in the normotensive subjects, which is higher than those reported in Germany (0.47) and Czech (0.40) populations, but similar to that found in Japanese populations (0.73). The frequencies of genotype AA, AG, and GG were 0.46, 0.49, and 0.05 in hypertensive subjects, and 0.44, 0.53, and 0.03 in control subjects. There is no significant difference in the distributions of the genotype and allele between the two groups (X2 = 0.88, P > 0.05; X2 = 0.024, P > 0.05). These findings differ from some of the results obtained in other ethnic groups, indicating the potential importance of ethnic origin in the assessment of genetic risk identifiers for a complex disease. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA.en_HK
dc.relation.ispartofElectrophoresisen_HK
dc.subjectAngiotensinogen geneen_HK
dc.subjectGenotypingen_HK
dc.subjectMicrochip electrophoresisen_HK
dc.subjectMiniaturizationen_HK
dc.subjectPolymorphism analysisen_HK
dc.titleGenotyping the -6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0173-0835&volume=26&spage=219&epage=224&date=2005&atitle=Genotyping+the+-6A/G+functional+polymorphism+in+the+core+promoter+region+of+angiotensinogen+gene+by+microchip+electrophoresisen_HK
dc.identifier.emailFung, Y:ysfung@hku.hken_HK
dc.identifier.authorityFung, Y=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/elps.200406158en_HK
dc.identifier.pmid15624175-
dc.identifier.scopuseid_2-s2.0-13444282463en_HK
dc.identifier.hkuros104846en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13444282463&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue1en_HK
dc.identifier.spage219en_HK
dc.identifier.epage224en_HK
dc.identifier.isiWOS:000226467400028-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridQin, J=7402895572en_HK
dc.identifier.scopusauthoridLiu, Z=37075585500en_HK
dc.identifier.scopusauthoridWu, D=36164763100en_HK
dc.identifier.scopusauthoridZhu, N=36923126600en_HK
dc.identifier.scopusauthoridZhou, X=10939365300en_HK
dc.identifier.scopusauthoridFung, Y=13309754700en_HK
dc.identifier.scopusauthoridLin, B=34668182800en_HK

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