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Article: Genome-wide biological response fingerprinting (BioReF) of the Chinese botanical formulation ISF-1 enables the selection of multiple marker genes as a potential metric for quality control

TitleGenome-wide biological response fingerprinting (BioReF) of the Chinese botanical formulation ISF-1 enables the selection of multiple marker genes as a potential metric for quality control
Authors
Issue Date2007
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal of Ethnopharmacology, 2007, v. 113 n. 1, p. 35-44 How to Cite?
AbstractQuality control plays a critical role in the process of translating the traditional/alternative medicines into modern evidence-based therapies. High performance liquid chromatography (HPLC) is widely applied to assess the chemical composition of botanical drug products. The chromatographic fingerprints or chemical profiles are currently used as the de facto quality control metric. As a complement to chemical profiles, a biological quality control assessment offers distinct advantages. This study describes a genome-wide biological response fingerprinting (BioReF) approach to define a set of marker genes that define a signature pattern for a specific botanical formulation. These marker genes are chosen on the basis of the levels of the regulated expression and the involvement in the cellular signaling pathways. Subsequently, qRT-PCR technique is used to simultaneously monitor the gene expression of multiple marker genes in an efficient and quantitative manner. This set of marker genes represents the biological responses of human cells to the chemical composition of the botanical drug that could serve as potential quality control of botanical drugs in terms of the consistency of biological activities. We demonstrate the BioReF approach with a well-documented Chinese Medicine formula, designated as ISF-1, traditionally used for the management of post-stroke disorders. A set of nine marker genes were selected to assess the batch-to-batch consistency of the biological effects of ISF-1. This approach provides a potential comprehensive and cost-effective quality control metric of the biological activities of botanical drugs.
Persistent Identifierhttp://hdl.handle.net/10722/68895
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRong, Jen_HK
dc.contributor.authorTilton, Ren_HK
dc.contributor.authorShen, Jen_HK
dc.contributor.authorNg, KMen_HK
dc.contributor.authorLiu, Cen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorLau, ASYen_HK
dc.contributor.authorCheng, YCen_HK
dc.date.accessioned2010-09-06T06:08:42Z-
dc.date.available2010-09-06T06:08:42Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal of Ethnopharmacology, 2007, v. 113 n. 1, p. 35-44en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68895-
dc.description.abstractQuality control plays a critical role in the process of translating the traditional/alternative medicines into modern evidence-based therapies. High performance liquid chromatography (HPLC) is widely applied to assess the chemical composition of botanical drug products. The chromatographic fingerprints or chemical profiles are currently used as the de facto quality control metric. As a complement to chemical profiles, a biological quality control assessment offers distinct advantages. This study describes a genome-wide biological response fingerprinting (BioReF) approach to define a set of marker genes that define a signature pattern for a specific botanical formulation. These marker genes are chosen on the basis of the levels of the regulated expression and the involvement in the cellular signaling pathways. Subsequently, qRT-PCR technique is used to simultaneously monitor the gene expression of multiple marker genes in an efficient and quantitative manner. This set of marker genes represents the biological responses of human cells to the chemical composition of the botanical drug that could serve as potential quality control of botanical drugs in terms of the consistency of biological activities. We demonstrate the BioReF approach with a well-documented Chinese Medicine formula, designated as ISF-1, traditionally used for the management of post-stroke disorders. A set of nine marker genes were selected to assess the batch-to-batch consistency of the biological effects of ISF-1. This approach provides a potential comprehensive and cost-effective quality control metric of the biological activities of botanical drugs.-
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshCost-Benefit Analysis-
dc.subject.meshDrugs, Chinese Herbal - standards-
dc.subject.meshGenes, Plant-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methods-
dc.titleGenome-wide biological response fingerprinting (BioReF) of the Chinese botanical formulation ISF-1 enables the selection of multiple marker genes as a potential metric for quality controlen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-8741&volume=113&spage=35&epage=44&date=2007&atitle=Genome-wide+Biological+Response+Fingerprinting+(BioReF)+of+the+Chinese+Botanical+Formulatrion+ISF-1+Enables+the+Selection+of+Multiple+Marker+Genes+as+a+Potential+Metric+for+Quality+Control+en_HK
dc.identifier.emailRong, J: jrong@hku.hken_HK
dc.identifier.emailShen, J: shenjg@hkucc.hku.hken_HK
dc.identifier.emailNg, KM: h9925586@graduate.hku.hken_HK
dc.identifier.emailLiu, C: changliu@hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.emailLau, ASY: asylau@hkucc.hku.hken_HK
dc.identifier.authorityRong, J=rp00515en_HK
dc.identifier.authorityShen, J=rp00487en_HK
dc.identifier.authorityNg, KM=rp00766en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityLau, ASY=rp00474en_HK
dc.identifier.doi10.1016/j.jep.2007.01.021-
dc.identifier.pmid17307317-
dc.identifier.scopuseid_2-s2.0-34447542716-
dc.identifier.hkuros135755en_HK
dc.identifier.volume113-
dc.identifier.issue1-
dc.identifier.spage35-
dc.identifier.epage44-
dc.identifier.eissn1872-7573-
dc.identifier.isiWOS:000249055200003-
dc.publisher.placeIreland-

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