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Article: Interactions of bismuth complexes with metallothionein(II)

TitleInteractions of bismuth complexes with metallothionein(II)
Authors
Issue Date1999
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1999, v. 274 n. 41, p. 29094-29101 How to Cite?
AbstractBismuth complexes are widely used as anti-ulcer drugs and can significantly reduce the side effects of platinum anti-cancer drugs. Bismuth is known to induce the synthesis of metallothionein (MT) in the kidney, but there are few chemical studies on the interactions of bismuth complexes with metallothionein. Here we show that Bi3+ binds strongly to metallothionein with a stoichiometry bismuth:MT = 7:1 (Bi7MT) and can readily displace Zn2+ and Cd2+. Bismuth is still bound to the protein even in strongly acidic solutions (pH 1). Reactions of bismuth citrate with MT are faster than those of [Bi(EDTA)]-, and both exhibit biphasic kinetics. 1H NMR data show that Zn2+ is displaced faster than Cd2+, and that both Zn2+ and Cd2+ in the β-domain (three metal cluster) of MT are displaced by Bi3+ much faster than from the α-domain (four metal cluster). The extended x-ray absorption fine structure spectrum of Bi7MT is very similar to that for the glutathione and N-acetyl-L-cysteine complexes [Bi(GS)3] and [Bi(NAC)3] with an inner coordination sphere of three sulfur atoms and average Bi-S distances of 2.55 Å. Some sites appear to contain additional short Bi-O bonds of 2.2 Å and longer Bi-S bonds of 3.1 Å. The Bi3+ sites in Bi7MT are therefore highly distorted in comparison with those of Zn2+ and Cd2+.
Persistent Identifierhttp://hdl.handle.net/10722/68826
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSun, Hen_HK
dc.contributor.authorLi, Hen_HK
dc.contributor.authorHarvey, Ien_HK
dc.contributor.authorSadler, PJen_HK
dc.date.accessioned2010-09-06T06:08:05Z-
dc.date.available2010-09-06T06:08:05Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Biological Chemistry, 1999, v. 274 n. 41, p. 29094-29101en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68826-
dc.description.abstractBismuth complexes are widely used as anti-ulcer drugs and can significantly reduce the side effects of platinum anti-cancer drugs. Bismuth is known to induce the synthesis of metallothionein (MT) in the kidney, but there are few chemical studies on the interactions of bismuth complexes with metallothionein. Here we show that Bi3+ binds strongly to metallothionein with a stoichiometry bismuth:MT = 7:1 (Bi7MT) and can readily displace Zn2+ and Cd2+. Bismuth is still bound to the protein even in strongly acidic solutions (pH 1). Reactions of bismuth citrate with MT are faster than those of [Bi(EDTA)]-, and both exhibit biphasic kinetics. 1H NMR data show that Zn2+ is displaced faster than Cd2+, and that both Zn2+ and Cd2+ in the β-domain (three metal cluster) of MT are displaced by Bi3+ much faster than from the α-domain (four metal cluster). The extended x-ray absorption fine structure spectrum of Bi7MT is very similar to that for the glutathione and N-acetyl-L-cysteine complexes [Bi(GS)3] and [Bi(NAC)3] with an inner coordination sphere of three sulfur atoms and average Bi-S distances of 2.55 Å. Some sites appear to contain additional short Bi-O bonds of 2.2 Å and longer Bi-S bonds of 3.1 Å. The Bi3+ sites in Bi7MT are therefore highly distorted in comparison with those of Zn2+ and Cd2+.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.titleInteractions of bismuth complexes with metallothionein(II)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=274&issue=41&spage=29094&epage=29101&date=1999&atitle=Interactions+of+bismuth+complexes+with+metallothionein(II)en_HK
dc.identifier.emailSun, H:hsun@hkucc.hku.hken_HK
dc.identifier.authoritySun, H=rp00777en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1074/jbc.274.41.29094en_HK
dc.identifier.pmid10506163-
dc.identifier.scopuseid_2-s2.0-0032855503en_HK
dc.identifier.hkuros50017en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032855503&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume274en_HK
dc.identifier.issue41en_HK
dc.identifier.spage29094en_HK
dc.identifier.epage29101en_HK
dc.identifier.isiWOS:000083017800042-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSun, H=7404827446en_HK
dc.identifier.scopusauthoridLi, H=14023043100en_HK
dc.identifier.scopusauthoridHarvey, I=35944665600en_HK
dc.identifier.scopusauthoridSadler, PJ=7103024488en_HK
dc.identifier.citeulike4721323-

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