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Article: Analytical application of acetate anion in negative electrospray ionization mass spectrometry for the analysis of triterpenoid saponins - Ginsenosides

TitleAnalytical application of acetate anion in negative electrospray ionization mass spectrometry for the analysis of triterpenoid saponins - Ginsenosides
Authors
Issue Date2006
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0951-4198/
Citation
Rapid Communications In Mass Spectrometry, 2006, v. 20 n. 10, p. 1545-1550 How to Cite?
AbstractGinsenosides containing different numbers of glycosyl groups can be easily differentiated based on the formation of characteristic ginsenoside-acetate adduct anions and deprotonated ginsenosides generated by electrospray ionization (ESI) of methanolic solutions of ginsenosides (M) and ammonium acetate (NH 4OAc). Ginsenosides containing two glycosyl groups gave a characteristic mass spectral pattern consisting of [M+2OAc] 2-, [M-H+OAc] 2- and [M-2H] 2- ions with m/z values differing by 30 Th, while this mass spectral pattern was not observed for ginsenosides containing one glycosyl group. Formation of [M+2OAc] 2- was influenced by the chain length of glycosyl groups and was used to differentiate the ginsenosides containing different combinations of monosaccharide and disaccharide units in the glycosyl groups. Under identical collisional activation conditions, [M+OAc] -, [M-H+OAc] 2- and [M+2OAc] 2- underwent proton abstractions predominantly to generate [M-H] -, [M-2H] 2- and [M-HH-OAc] 2- ions, respectively. The ion intensity ratios, I [M-H]-/I [M+OAc]-, I [M-2H]2-/I [M-H+2OAc]2- and I [M-H+OAc]2-/ I [M+OAc]2-, being sensitive to the structural differences of ginsenosides, could differentiate the isomeric ginsenosides, including (i) Rf, F 11 and Rg 1, (ii) Rd and Re, and (iii) Rb 2 and Rc. Additionally, NH 4OAc was found to enhance the sensitivity of detection of ginsenosides in the form of [M-H] - down to the femtomole level. Copyright © 2006 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/68801
ISSN
2015 Impact Factor: 2.226
2015 SCImago Journal Rankings: 0.819
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, KMen_HK
dc.contributor.authorChe, CMen_HK
dc.contributor.authorWo, SKen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorLau, ASYen_HK
dc.date.accessioned2010-09-06T06:07:51Z-
dc.date.available2010-09-06T06:07:51Z-
dc.date.issued2006en_HK
dc.identifier.citationRapid Communications In Mass Spectrometry, 2006, v. 20 n. 10, p. 1545-1550en_HK
dc.identifier.issn0951-4198en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68801-
dc.description.abstractGinsenosides containing different numbers of glycosyl groups can be easily differentiated based on the formation of characteristic ginsenoside-acetate adduct anions and deprotonated ginsenosides generated by electrospray ionization (ESI) of methanolic solutions of ginsenosides (M) and ammonium acetate (NH 4OAc). Ginsenosides containing two glycosyl groups gave a characteristic mass spectral pattern consisting of [M+2OAc] 2-, [M-H+OAc] 2- and [M-2H] 2- ions with m/z values differing by 30 Th, while this mass spectral pattern was not observed for ginsenosides containing one glycosyl group. Formation of [M+2OAc] 2- was influenced by the chain length of glycosyl groups and was used to differentiate the ginsenosides containing different combinations of monosaccharide and disaccharide units in the glycosyl groups. Under identical collisional activation conditions, [M+OAc] -, [M-H+OAc] 2- and [M+2OAc] 2- underwent proton abstractions predominantly to generate [M-H] -, [M-2H] 2- and [M-HH-OAc] 2- ions, respectively. The ion intensity ratios, I [M-H]-/I [M+OAc]-, I [M-2H]2-/I [M-H+2OAc]2- and I [M-H+OAc]2-/ I [M+OAc]2-, being sensitive to the structural differences of ginsenosides, could differentiate the isomeric ginsenosides, including (i) Rf, F 11 and Rg 1, (ii) Rd and Re, and (iii) Rb 2 and Rc. Additionally, NH 4OAc was found to enhance the sensitivity of detection of ginsenosides in the form of [M-H] - down to the femtomole level. Copyright © 2006 John Wiley & Sons, Ltd.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0951-4198/en_HK
dc.relation.ispartofRapid Communications in Mass Spectrometryen_HK
dc.rightsRapid Communications in Mass Spectrometry. Copyright © John Wiley & Sons Ltd.en_HK
dc.titleAnalytical application of acetate anion in negative electrospray ionization mass spectrometry for the analysis of triterpenoid saponins - Ginsenosidesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0951-4198&volume=20&spage=1545 &epage= 1550&date=2006&atitle=Analytical+Application+of+Acetate+Anion+in+Negative+Electrospray+Ionization+Mass+Spectrometry+for+the+Analysis+of+Triterpenoid+Saponins+-+Ginsenosides+en_HK
dc.identifier.emailNg, KM:kwanmng@hku.hken_HK
dc.identifier.emailChe, CM:cmche@hku.hken_HK
dc.identifier.emailTam, PKH:paultam@hkucc.hku.hken_HK
dc.identifier.emailLau, ASY:asylau@hku.hken_HK
dc.identifier.authorityNg, KM=rp00766en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityLau, ASY=rp00474en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/rcm.2465en_HK
dc.identifier.pmid16628596-
dc.identifier.scopuseid_2-s2.0-33646752113en_HK
dc.identifier.hkuros113879en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646752113&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1545en_HK
dc.identifier.epage1550en_HK
dc.identifier.isiWOS:000237794300012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridNg, KM=26026091100en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.scopusauthoridWo, SK=6701683232en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK
dc.identifier.scopusauthoridLau, ASY=7202626202en_HK

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