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Article: Separation and determination of the antitumor drug piritrexim by molecularly imprinted microspheres in high-performance liquid chromatography

TitleSeparation and determination of the antitumor drug piritrexim by molecularly imprinted microspheres in high-performance liquid chromatography
Authors
KeywordsAqueous microsuspension polymerisation
Chromatographic stationary phase
Molecularly imprinted microspheres
Piritrexim
Trimetrexate
Issue Date2003
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
Citation
Analytical And Bioanalytical Chemistry, 2003, v. 377 n. 1, p. 208-213 How to Cite?
AbstractMolecularly imprinted microspheres were synthesised using the antitumor drug piritrexim (PTX) as a template molecule by aqueous microsuspension polymerisation and were used as a high-performance liquid chromatographic stationary phase. The molecularly imprinted column exhibited strong retention behaviour to the template molecule. The influences of pH of the buffer and the ratio of methanol to buffer on the retention behaviour were investigated in detail. Results indicated that the baseline separation of PTX, trimetrexate (TMX), trimethoprim (TMP) and sulfamethazine (SMZ) was achieved when the pH value of the acetate buffer was above pH 3.5 and the ratio of methanol to the buffer was 6:4 (v/v). A gradient elution programme was employed to enhance the separation, which led to an improvement in sensitivity and a reduction in determination errors. The method developed was used to analyse urine samples supplemented with PTX. The recoveries of 5 μg mL-1 PTX in the urine sample were 99.1±3.0% and 93.3±2.8% at the beginning and 24h later, respectively. © Springer-Verlag 2003.
Persistent Identifierhttp://hdl.handle.net/10722/68744
ISSN
2015 Impact Factor: 3.125
2015 SCImago Journal Rankings: 1.096
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, JPen_HK
dc.contributor.authorHe, XWen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:07:15Z-
dc.date.available2010-09-06T06:07:15Z-
dc.date.issued2003en_HK
dc.identifier.citationAnalytical And Bioanalytical Chemistry, 2003, v. 377 n. 1, p. 208-213en_HK
dc.identifier.issn1618-2642en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68744-
dc.description.abstractMolecularly imprinted microspheres were synthesised using the antitumor drug piritrexim (PTX) as a template molecule by aqueous microsuspension polymerisation and were used as a high-performance liquid chromatographic stationary phase. The molecularly imprinted column exhibited strong retention behaviour to the template molecule. The influences of pH of the buffer and the ratio of methanol to buffer on the retention behaviour were investigated in detail. Results indicated that the baseline separation of PTX, trimetrexate (TMX), trimethoprim (TMP) and sulfamethazine (SMZ) was achieved when the pH value of the acetate buffer was above pH 3.5 and the ratio of methanol to the buffer was 6:4 (v/v). A gradient elution programme was employed to enhance the separation, which led to an improvement in sensitivity and a reduction in determination errors. The method developed was used to analyse urine samples supplemented with PTX. The recoveries of 5 μg mL-1 PTX in the urine sample were 99.1±3.0% and 93.3±2.8% at the beginning and 24h later, respectively. © Springer-Verlag 2003.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htmen_HK
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_HK
dc.subjectAqueous microsuspension polymerisationen_HK
dc.subjectChromatographic stationary phaseen_HK
dc.subjectMolecularly imprinted microspheresen_HK
dc.subjectPiritreximen_HK
dc.subjectTrimetrexateen_HK
dc.titleSeparation and determination of the antitumor drug piritrexim by molecularly imprinted microspheres in high-performance liquid chromatographyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1618-2642&volume=377&issue=1&spage=208&epage=213&date=2003&atitle=Separation+and+determination+of+the+antitumor+drug+piritrexim+by+molecularly+imprinted+microspheres+in+high+performance+liquid+chromatographyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00216-003-2086-8en_HK
dc.identifier.pmid12845402-
dc.identifier.scopuseid_2-s2.0-1542652190en_HK
dc.identifier.hkuros90963en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542652190&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume377en_HK
dc.identifier.issue1en_HK
dc.identifier.spage208en_HK
dc.identifier.epage213en_HK
dc.identifier.isiWOS:000185423200030-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLai, JP=7401939703en_HK
dc.identifier.scopusauthoridHe, XW=7404409073en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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