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Article: Production of rosmarinic acid and lithospermic acid B in Ti transformed Salvia miltiorrhiza cell suspension cultures

TitleProduction of rosmarinic acid and lithospermic acid B in Ti transformed Salvia miltiorrhiza cell suspension cultures
Authors
KeywordsBioreactor
Conductivity
Initial pH
Lithosperrnic acid B
Rosmarinic acid
Salvia miltiorrhiza
Ti transformed cell culture
Issue Date1999
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio
Citation
Process Biochemistry, 1999, v. 34 n. 8, p. 777-784 How to Cite?
AbstractA Ti transformed cell culture of S. miltiorrhiza demonstrated the ability to produce rosmarinic acid (RA) and lithospermic acid B (LAB) as determined by HPLC. A time course of biomass accumulation, phenolic compound formation, medium pH and conductivity as well as sugar, nitrate, ammonium and phosphate uptake was examined both in flask and in bioreactor cultures using hormone free B5 medium containing 2% sucrose. In flasks, the stationary phase of growth was reached after about 16 days of cultivation; the maximum biomass concentration was about 10.5 g l-1 dry weight. The maximum production of RA (4.59% of dry cell weight or 481.5 mg l-1) appeared on the 20th day of cultivation, at which time the production of LAB was 0.81% of dry weight or 85.0 mg l-1. A linear relationship with an intercept between the dry cell weight increase and the medium conductivity decrease was observed which may be used for monitoring cell growth rate. Initial medium pH over a wide range (3.4-8.5) had little effect on cell growth but had significant influences on rosmarinic acid formation, although the trend was irregular. In bioreactor cultivation, the maximum dry Cell biomass accumulation obtained was 7.52 g l-1 on the 15th day of cultivation. The lower performance of cell growth in the bioreactor was due to the poor mixing of the culture.
Persistent Identifierhttp://hdl.handle.net/10722/68737
ISSN
2015 Impact Factor: 2.529
2015 SCImago Journal Rankings: 0.937
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Hen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorZhang, YLen_HK
dc.contributor.authorSong, JYen_HK
dc.date.accessioned2010-09-06T06:07:11Z-
dc.date.available2010-09-06T06:07:11Z-
dc.date.issued1999en_HK
dc.identifier.citationProcess Biochemistry, 1999, v. 34 n. 8, p. 777-784en_HK
dc.identifier.issn1359-5113en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68737-
dc.description.abstractA Ti transformed cell culture of S. miltiorrhiza demonstrated the ability to produce rosmarinic acid (RA) and lithospermic acid B (LAB) as determined by HPLC. A time course of biomass accumulation, phenolic compound formation, medium pH and conductivity as well as sugar, nitrate, ammonium and phosphate uptake was examined both in flask and in bioreactor cultures using hormone free B5 medium containing 2% sucrose. In flasks, the stationary phase of growth was reached after about 16 days of cultivation; the maximum biomass concentration was about 10.5 g l-1 dry weight. The maximum production of RA (4.59% of dry cell weight or 481.5 mg l-1) appeared on the 20th day of cultivation, at which time the production of LAB was 0.81% of dry weight or 85.0 mg l-1. A linear relationship with an intercept between the dry cell weight increase and the medium conductivity decrease was observed which may be used for monitoring cell growth rate. Initial medium pH over a wide range (3.4-8.5) had little effect on cell growth but had significant influences on rosmarinic acid formation, although the trend was irregular. In bioreactor cultivation, the maximum dry Cell biomass accumulation obtained was 7.52 g l-1 on the 15th day of cultivation. The lower performance of cell growth in the bioreactor was due to the poor mixing of the culture.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbioen_HK
dc.relation.ispartofProcess Biochemistryen_HK
dc.rightsProcess Biochemistry. Copyright © Elsevier Ltd.en_HK
dc.subjectBioreactoren_HK
dc.subjectConductivityen_HK
dc.subjectInitial pHen_HK
dc.subjectLithosperrnic acid Ben_HK
dc.subjectRosmarinic aciden_HK
dc.subjectSalvia miltiorrhizaen_HK
dc.subjectTi transformed cell cultureen_HK
dc.titleProduction of rosmarinic acid and lithospermic acid B in Ti transformed Salvia miltiorrhiza cell suspension culturesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1359-5113&volume=34&spage=777&epage=784&date=1999&atitle=Production+of+rosmarinic+acid+and+lithospermic+acid+B+in+Ti+transformed+Salvia+miltiorrhiza+cell+suspension+culturesen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0032-9592(98)00155-1en_HK
dc.identifier.scopuseid_2-s2.0-0032867519en_HK
dc.identifier.hkuros52975en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032867519&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue8en_HK
dc.identifier.spage777en_HK
dc.identifier.epage784en_HK
dc.identifier.isiWOS:000083166300002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChen, H=26642984400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridZhang, YL=7601313628en_HK
dc.identifier.scopusauthoridSong, JY=7404788369en_HK

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