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Article: Optimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis

TitleOptimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis
Authors
KeywordsDiatom
EPA
Heterotrophic growth
Nitrogen source
Nitzschia laevis
Issue Date2001
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictec
Citation
Enzyme And Microbial Technology, 2001, v. 29 n. 6-7, p. 341-347 How to Cite?
AbstractThe nitrogen (N) sources including nitrate, ammonium, urea, tryptone and yeast extract were optimized for eicosapentaenoic acid (EPA) production by the diatom Nitzschia laevis in heterotrophic cultures. First, nitrate and urea were found to be the preferred N sources for both cell growth and EPA content. The optimal concentrations of nitrate and urea for cell growth and EPA yield were 620 mg/liter and 600 mg/liter, respectively. Secondly, tryptone and yeast extract were respectively added to the medium and both of them were found to enhance EPA production compared with the control. To investigate the co-effects of tryptone and yeast extract, in the subsequent experiments, a central composite experimental design was used to optimize the two complex nitrogen sources. The results showed that the EPA production was influenced not only by tryptone or yeast extract, but also by their interactions. The cell dry weight (DW), EPA content and EPA yield could be described by the second-order polynomial equations with high confidence levels (>99%). Based on the surface plots of the responses, the optimal concentrations of tryptone and yeast extract were determined to be 1.6 g/liter and 0.8 g/liter, respectively. To verify the predicted models, the alga was grown in the medium with tryptone and yeast extract at optimal concentrations. The resulting DW, EPA content and EPA yield reached 6.48 g/liter, 2.74% (w/w), and 175 mg/liter, respectively, which agreed with the predicted values, and were much higher than those obtained in the previous studies. © 2001 Elsevier Science Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68734
ISSN
2015 Impact Factor: 2.624
2015 SCImago Journal Rankings: 0.854
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWen, ZYen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:07:10Z-
dc.date.available2010-09-06T06:07:10Z-
dc.date.issued2001en_HK
dc.identifier.citationEnzyme And Microbial Technology, 2001, v. 29 n. 6-7, p. 341-347en_HK
dc.identifier.issn0141-0229en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68734-
dc.description.abstractThe nitrogen (N) sources including nitrate, ammonium, urea, tryptone and yeast extract were optimized for eicosapentaenoic acid (EPA) production by the diatom Nitzschia laevis in heterotrophic cultures. First, nitrate and urea were found to be the preferred N sources for both cell growth and EPA content. The optimal concentrations of nitrate and urea for cell growth and EPA yield were 620 mg/liter and 600 mg/liter, respectively. Secondly, tryptone and yeast extract were respectively added to the medium and both of them were found to enhance EPA production compared with the control. To investigate the co-effects of tryptone and yeast extract, in the subsequent experiments, a central composite experimental design was used to optimize the two complex nitrogen sources. The results showed that the EPA production was influenced not only by tryptone or yeast extract, but also by their interactions. The cell dry weight (DW), EPA content and EPA yield could be described by the second-order polynomial equations with high confidence levels (>99%). Based on the surface plots of the responses, the optimal concentrations of tryptone and yeast extract were determined to be 1.6 g/liter and 0.8 g/liter, respectively. To verify the predicted models, the alga was grown in the medium with tryptone and yeast extract at optimal concentrations. The resulting DW, EPA content and EPA yield reached 6.48 g/liter, 2.74% (w/w), and 175 mg/liter, respectively, which agreed with the predicted values, and were much higher than those obtained in the previous studies. © 2001 Elsevier Science Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictecen_HK
dc.relation.ispartofEnzyme and Microbial Technologyen_HK
dc.rightsEnzyme and Microbial Technology. Copyright © Elsevier Inc.en_HK
dc.subjectDiatomen_HK
dc.subjectEPAen_HK
dc.subjectHeterotrophic growthen_HK
dc.subjectNitrogen sourceen_HK
dc.subjectNitzschia laevisen_HK
dc.titleOptimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-0229&volume=29&spage=341&epage=347&date=2001&atitle=Optimization+of+nitrogen+sources+for+heterotrophic+production+of+eicosapentaenoic+acid+by+the+diatom+Nitzschia+laevisen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0141-0229(01)00385-4en_HK
dc.identifier.scopuseid_2-s2.0-0035807431en_HK
dc.identifier.hkuros72425en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035807431&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue6-7en_HK
dc.identifier.spage341en_HK
dc.identifier.epage347en_HK
dc.identifier.isiWOS:000171480100004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWen, ZY=14012373200en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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