File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of TSCL, a nonviral retroposon from Arabidopsis thaliana

TitleCharacterization of TSCL, a nonviral retroposon from Arabidopsis thaliana
Authors
KeywordsCortex
Cruciferae
Genome
In situ hybridization
Retroelements
Sclerenchyma
Issue Date1997
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412
Citation
Plant Molecular Biology, 1997, v. 35 n. 6, p. 893-904 How to Cite?
AbstractWe isolated by differential screening a 1.2 kb cDNA from an Arabidopsis thaliana ecotype Columbia cDNA library that is highly expressed in stem and root. In situ hybridization studies on stem sections and root sections showed that the mRNA is expressed in stem sclerenchyma and root cortex, respectively. The isolation and sequence analysis of four other overlapping cDNA clones from two independent A. thaliana cDNA libraries confirmed that these cDNAs lack a significant open reading frame that has recognizable homology to any known proteins. We have obtained from A. thaliana ecotype Columbia three corresponding genomic clones and nucleotide sequence analysis of these clones revealed that we have isolated a retroposon, TSCL, that is flanked by two 13 bp direct repeats, is intronless, and has a poly(A)+ tract at the 3' end. The site of transcription initiation mapped by primer extension analysis lies 48 bp downstream from an external TATA box. Results from Southern blot analysis suggest that TSCL occurs as a single-copy insert in the genomes of A. thaliana ecotype Columbia (Col-0) and Col-2 but is absent in the genomes of Brassica napus, Brassica juncea and A. thaliana ecotypes Be-0, Oy-0 and Ler-0. This suggests that Col-0 and Col-2 are phylogenetically more closely related to each other than to Be-0, Oy-0 and Let-0, and that the Laibach Landsberg seeds Redei received, from which ecotypes Col-0, Col-2 and Ler-0 originated, were heterogeneous for TSCL.
Persistent Identifierhttp://hdl.handle.net/10722/68732
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 1.915
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChye, MLen_HK
dc.contributor.authorCheung, KYen_HK
dc.contributor.authorXu, Jen_HK
dc.date.accessioned2010-09-06T06:07:09Z-
dc.date.available2010-09-06T06:07:09Z-
dc.date.issued1997en_HK
dc.identifier.citationPlant Molecular Biology, 1997, v. 35 n. 6, p. 893-904en_HK
dc.identifier.issn0167-4412en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68732-
dc.description.abstractWe isolated by differential screening a 1.2 kb cDNA from an Arabidopsis thaliana ecotype Columbia cDNA library that is highly expressed in stem and root. In situ hybridization studies on stem sections and root sections showed that the mRNA is expressed in stem sclerenchyma and root cortex, respectively. The isolation and sequence analysis of four other overlapping cDNA clones from two independent A. thaliana cDNA libraries confirmed that these cDNAs lack a significant open reading frame that has recognizable homology to any known proteins. We have obtained from A. thaliana ecotype Columbia three corresponding genomic clones and nucleotide sequence analysis of these clones revealed that we have isolated a retroposon, TSCL, that is flanked by two 13 bp direct repeats, is intronless, and has a poly(A)+ tract at the 3' end. The site of transcription initiation mapped by primer extension analysis lies 48 bp downstream from an external TATA box. Results from Southern blot analysis suggest that TSCL occurs as a single-copy insert in the genomes of A. thaliana ecotype Columbia (Col-0) and Col-2 but is absent in the genomes of Brassica napus, Brassica juncea and A. thaliana ecotypes Be-0, Oy-0 and Ler-0. This suggests that Col-0 and Col-2 are phylogenetically more closely related to each other than to Be-0, Oy-0 and Let-0, and that the Laibach Landsberg seeds Redei received, from which ecotypes Col-0, Col-2 and Ler-0 originated, were heterogeneous for TSCL.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412en_HK
dc.relation.ispartofPlant Molecular Biologyen_HK
dc.subjectCortexen_HK
dc.subjectCruciferaeen_HK
dc.subjectGenomeen_HK
dc.subjectIn situ hybridizationen_HK
dc.subjectRetroelementsen_HK
dc.subjectSclerenchymaen_HK
dc.titleCharacterization of TSCL, a nonviral retroposon from Arabidopsis thalianaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-4412&volume=35&spage=893&epage=903&date=1997&atitle=Characterization+of+TSCL,+a+nonviral+retroposon+from+Arabidopsis+thalianaen_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1005947804227en_HK
dc.identifier.pmid9426608-
dc.identifier.scopuseid_2-s2.0-0031440663en_HK
dc.identifier.hkuros35735en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031440663&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue6en_HK
dc.identifier.spage893en_HK
dc.identifier.epage904en_HK
dc.identifier.isiWOS:000070980300019-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK
dc.identifier.scopusauthoridCheung, KY=36893099700en_HK
dc.identifier.scopusauthoridXu, J=7407005993en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats