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Article: Induction of apoptosis by 1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3- propanedione through reactive oxygen species production, GADD153 expression, and caspases activation in human epidermoid carcinoma cells

TitleInduction of apoptosis by 1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3- propanedione through reactive oxygen species production, GADD153 expression, and caspases activation in human epidermoid carcinoma cells
Authors
KeywordsAntioxidant
Apoptosis
Caspase-3
Caspase-9
Cytochrome c
GADD153
HMDB
Poly(ADP-ribose) polymerase
Reactive oxygen species
Issue Date2005
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 2005, v. 53 n. 23, p. 9039-9049 How to Cite?
AbstractThis study examined the growth inhibitory effects of the structurally related β-diketones compounds in human cancer cells. Here, we report that 1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione (HMDB) induces growth inhibition of human cancer cells and induction of apoptosis in A431 cells through modulation of mitochondrial functions regulated by reactive oxygen species (ROS). ROS generation occurs in the early stages of HMDB-induced apoptosis, preceding cytochrome c release, caspase activation, and DNA fragmentation. The changes occurred after single breaks in DNA were detected, suggesting that HMDB induced irreparable DNA damage, which in turn triggered the process of apoptosis. Up-regulation of Bad and p21; down-regulation of Bcl-2, Bcl-X L, Bid, p53, and fatty acid synthase; and cleavage of Bax were found in HMDB-treated A431 cells. Glutathione and N-acetylcysteine (NAC) suppress HMDB-induced apoptosis. HMDB markedly enhanced growth arrest DNA damage inducible gene 153 (GADD153) mRNA and protein in a time- and concentration-dependent manner. NAC prevented up-regulation of GADD153 mRNA expression caused by HMDB. These findings suggest that HMDB creates an oxidative cellular environment that induces DNA damage and GADD153 gene activation, which in turn helps trigger apoptosis in A431 cells. © 2005 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/68731
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPan, MHen_HK
dc.contributor.authorSin, YHen_HK
dc.contributor.authorLai, CSen_HK
dc.contributor.authorWang, YJen_HK
dc.contributor.authorLin, JKen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorHo, CTen_HK
dc.date.accessioned2010-09-06T06:07:08Z-
dc.date.available2010-09-06T06:07:08Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2005, v. 53 n. 23, p. 9039-9049en_HK
dc.identifier.issn0021-8561en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68731-
dc.description.abstractThis study examined the growth inhibitory effects of the structurally related β-diketones compounds in human cancer cells. Here, we report that 1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione (HMDB) induces growth inhibition of human cancer cells and induction of apoptosis in A431 cells through modulation of mitochondrial functions regulated by reactive oxygen species (ROS). ROS generation occurs in the early stages of HMDB-induced apoptosis, preceding cytochrome c release, caspase activation, and DNA fragmentation. The changes occurred after single breaks in DNA were detected, suggesting that HMDB induced irreparable DNA damage, which in turn triggered the process of apoptosis. Up-regulation of Bad and p21; down-regulation of Bcl-2, Bcl-X L, Bid, p53, and fatty acid synthase; and cleavage of Bax were found in HMDB-treated A431 cells. Glutathione and N-acetylcysteine (NAC) suppress HMDB-induced apoptosis. HMDB markedly enhanced growth arrest DNA damage inducible gene 153 (GADD153) mRNA and protein in a time- and concentration-dependent manner. NAC prevented up-regulation of GADD153 mRNA expression caused by HMDB. These findings suggest that HMDB creates an oxidative cellular environment that induces DNA damage and GADD153 gene activation, which in turn helps trigger apoptosis in A431 cells. © 2005 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_HK
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_HK
dc.subjectAntioxidanten_HK
dc.subjectApoptosisen_HK
dc.subjectCaspase-3en_HK
dc.subjectCaspase-9en_HK
dc.subjectCytochrome cen_HK
dc.subjectGADD153en_HK
dc.subjectHMDBen_HK
dc.subjectPoly(ADP-ribose) polymeraseen_HK
dc.subjectReactive oxygen speciesen_HK
dc.titleInduction of apoptosis by 1-(2-hydroxy-5-methylphenyl)-3-phenyl-1,3- propanedione through reactive oxygen species production, GADD153 expression, and caspases activation in human epidermoid carcinoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8561&volume=53&issue=23&spage=9039&epage=9049&date=2005&atitle=Induction+of+Apoptosis+by+1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione+through+Reactive+Oxygen+Species+Production,+GADD153+Expression,+and+Caspases+Activation+in+Human+Epidermoid+Carcinoma+Cellsen_HK
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/jf051476aen_HK
dc.identifier.pmid16277400-
dc.identifier.scopuseid_2-s2.0-28444437455en_HK
dc.identifier.hkuros121148en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-28444437455&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue23en_HK
dc.identifier.spage9039en_HK
dc.identifier.epage9049en_HK
dc.identifier.isiWOS:000233306700025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPan, MH=7202544934en_HK
dc.identifier.scopusauthoridSin, YH=37045603400en_HK
dc.identifier.scopusauthoridLai, CS=8744893900en_HK
dc.identifier.scopusauthoridWang, YJ=35081383400en_HK
dc.identifier.scopusauthoridLin, JK=35068241700en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridHo, CT=7404652573en_HK

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