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Article: Preparative purification of glycyrrhizin extracted from the root of liquorice using high-speed counter-current chromatography

TitlePreparative purification of glycyrrhizin extracted from the root of liquorice using high-speed counter-current chromatography
Authors
KeywordsCounter-current chromatography
Glycyrrhiza uralensis
Glycyrrhizin
Pharmaceutical analysis
Plant materials
Preparative chromatography
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2004, v. 1033 n. 1, p. 183-186 How to Cite?
AbstractGlycyrrhizin is one of the main bioactive components in liquorice (Glycyrrhiza uralensis Fisch) which has recently been found to be highly active in inhibiting replication of the severe acute respiratory syndrome (SARS)-associated virus. The separation and purification of glycyrrhizin from a methanol-water (70:30 (v/v)) extract of liquorice roots was achieved using high-speed counter-current chromatography. The separation was performed at a preparative scale in a one-step separation with a two-phase solvent system composed of ethyl acetate-methanol-water (5:2:5 (v/v)). The lower phase was used as the mobile phase in the head-to-tail elution mode. The present method yielded 42.2mg glycyrrhizin at 96.8% purity from 130mg of the crude exact with 95.2% recovery as determined by HPLC analysis. © 2004 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68724
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorLu, HTen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:07:05Z-
dc.date.available2010-09-06T06:07:05Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Chromatography A, 2004, v. 1033 n. 1, p. 183-186en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68724-
dc.description.abstractGlycyrrhizin is one of the main bioactive components in liquorice (Glycyrrhiza uralensis Fisch) which has recently been found to be highly active in inhibiting replication of the severe acute respiratory syndrome (SARS)-associated virus. The separation and purification of glycyrrhizin from a methanol-water (70:30 (v/v)) extract of liquorice roots was achieved using high-speed counter-current chromatography. The separation was performed at a preparative scale in a one-step separation with a two-phase solvent system composed of ethyl acetate-methanol-water (5:2:5 (v/v)). The lower phase was used as the mobile phase in the head-to-tail elution mode. The present method yielded 42.2mg glycyrrhizin at 96.8% purity from 130mg of the crude exact with 95.2% recovery as determined by HPLC analysis. © 2004 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectGlycyrrhiza uralensisen_HK
dc.subjectGlycyrrhizinen_HK
dc.subjectPharmaceutical analysisen_HK
dc.subjectPlant materialsen_HK
dc.subjectPreparative chromatographyen_HK
dc.titlePreparative purification of glycyrrhizin extracted from the root of liquorice using high-speed counter-current chromatographyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1033&issue=1&spage=183&epage=186&date=2004&atitle=Preparative+purification+of+glycyrrhizin+extracted+from+the+root+of+liquorice+using+high-speed+counter-current+chromatographyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2004.01.033en_HK
dc.identifier.pmid15072305-
dc.identifier.scopuseid_2-s2.0-1542298896en_HK
dc.identifier.hkuros90881en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542298896&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1033en_HK
dc.identifier.issue1en_HK
dc.identifier.spage183en_HK
dc.identifier.epage186en_HK
dc.identifier.isiWOS:000220290100021-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK
dc.identifier.scopusauthoridLu, HT=7404843255en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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