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Article: Production of lithospermic acid B and rosmarinic acid in hairy root cultures of Salvia miltiorrhiza

TitleProduction of lithospermic acid B and rosmarinic acid in hairy root cultures of Salvia miltiorrhiza
Authors
KeywordsAgrobacterium rhizogenes
Hairy roots
Lamiaceae
Lithospermic acid B
Rosmarinic acid
Salvia miltiorrhiza
Issue Date1999
PublisherSpringer Verlag.
Citation
Journal Of Industrial Microbiology And Biotechnology, 1999, v. 22 n. 3, p. 133-138 How to Cite?
AbstractHairy root cultures of Salvia miltiorrhiza were established by infecting sterile plantlets with Agrobacterium rhizogenes ATCC 15834, and the transformation was proved by direct detection of the inserted T-DNA by the polymerase chain reaction. As determined by HPLC, these hairy root cultures had the ability to produce lithospermic acid B (LAB), rosmarinic acid (RA) and other related phenolic compounds, the water-soluble active components of the plant. The effect of five different basal media, MS, MS-NH 4 (MS without ammonium nitrate), B5, WPM and 6,7-V on the root growth and phenolic compound production was studied. It was found that MS-NH 4, and 6,7-V media were superior to MS, B5 and WPM media in terms of both root growth and phenolic compound production. The time course of biomass accumulation and phenolic compound formation was also examined in the culture using MS-NH 4, medium. During cultivation, the content of RA in the roots was stable being approximately 0.48% of dry weight while the content of LAB fluctuated between 0.73% and 1.61% of dry weight, and decreased gradually at the stationary phase of growth. The highest production of LAB and RA was about 64 mg L -1 and 23 mg L -1, respectively.
Persistent Identifierhttp://hdl.handle.net/10722/68712
ISSN
2015 Impact Factor: 2.745
2015 SCImago Journal Rankings: 0.966
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Hen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorZhang, YLen_HK
dc.contributor.authorSong, JYen_HK
dc.date.accessioned2010-09-06T06:06:59Z-
dc.date.available2010-09-06T06:06:59Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Industrial Microbiology And Biotechnology, 1999, v. 22 n. 3, p. 133-138en_HK
dc.identifier.issn1367-5435en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68712-
dc.description.abstractHairy root cultures of Salvia miltiorrhiza were established by infecting sterile plantlets with Agrobacterium rhizogenes ATCC 15834, and the transformation was proved by direct detection of the inserted T-DNA by the polymerase chain reaction. As determined by HPLC, these hairy root cultures had the ability to produce lithospermic acid B (LAB), rosmarinic acid (RA) and other related phenolic compounds, the water-soluble active components of the plant. The effect of five different basal media, MS, MS-NH 4 (MS without ammonium nitrate), B5, WPM and 6,7-V on the root growth and phenolic compound production was studied. It was found that MS-NH 4, and 6,7-V media were superior to MS, B5 and WPM media in terms of both root growth and phenolic compound production. The time course of biomass accumulation and phenolic compound formation was also examined in the culture using MS-NH 4, medium. During cultivation, the content of RA in the roots was stable being approximately 0.48% of dry weight while the content of LAB fluctuated between 0.73% and 1.61% of dry weight, and decreased gradually at the stationary phase of growth. The highest production of LAB and RA was about 64 mg L -1 and 23 mg L -1, respectively.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag.en_HK
dc.relation.ispartofJournal of Industrial Microbiology and Biotechnologyen_HK
dc.subjectAgrobacterium rhizogenesen_HK
dc.subjectHairy rootsen_HK
dc.subjectLamiaceaeen_HK
dc.subjectLithospermic acid Ben_HK
dc.subjectRosmarinic aciden_HK
dc.subjectSalvia miltiorrhizaen_HK
dc.titleProduction of lithospermic acid B and rosmarinic acid in hairy root cultures of Salvia miltiorrhizaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1367-5435&volume=22&spage=133&epage=138&date=1999&atitle=Production+of+lithospermic+acid+B+and+rosmarinic+acid+in+hairy+root+cultures+of+Salvia+miltiorrhizaen_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/sj.jim.2900624en_HK
dc.identifier.scopuseid_2-s2.0-0033005013en_HK
dc.identifier.hkuros45272en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033005013&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue3en_HK
dc.identifier.spage133en_HK
dc.identifier.epage138en_HK
dc.identifier.isiWOS:000080883900002-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridZhang, YL=7601313628en_HK
dc.identifier.scopusauthoridSong, JY=7404788369en_HK

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