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Article: Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography

TitleIsolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography
Authors
KeywordsCarotenoids
Counter-current chromatography
Microcystis aeruginosa
Preparative chromatography
Zeaxanthin
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2005, v. 1064 n. 2, p. 183-186 How to Cite?
AbstractHigh-speed counter-current chromatography was successfully applied for the first time to the isolation and purification of the bioactive carotenoid zeaxanthin from the cyanobacterium Microcystis aeruginosa. The crude zeaxanthin was obtained by extraction with organic solvents after the microalgal sample had been saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (8:2:7:3, v/v/v/v) was successfully performed yielding zeaxanthin at 96.2% purity from 150 mg of the crude extract in a one-step separation. The recovery of zeaxanthin was 91.4%. This was also the first report that zeaxanthin was successfully separated and purified from microalgae. © 2004 Published by Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/68688
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Fen_HK
dc.contributor.authorLi, HBen_HK
dc.contributor.authorWong, RNSen_HK
dc.contributor.authorJi, Ben_HK
dc.contributor.authorJiang, Yen_HK
dc.date.accessioned2010-09-06T06:06:46Z-
dc.date.available2010-09-06T06:06:46Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Chromatography A, 2005, v. 1064 n. 2, p. 183-186en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68688-
dc.description.abstractHigh-speed counter-current chromatography was successfully applied for the first time to the isolation and purification of the bioactive carotenoid zeaxanthin from the cyanobacterium Microcystis aeruginosa. The crude zeaxanthin was obtained by extraction with organic solvents after the microalgal sample had been saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (8:2:7:3, v/v/v/v) was successfully performed yielding zeaxanthin at 96.2% purity from 150 mg of the crude extract in a one-step separation. The recovery of zeaxanthin was 91.4%. This was also the first report that zeaxanthin was successfully separated and purified from microalgae. © 2004 Published by Elsevier B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectCarotenoidsen_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectMicrocystis aeruginosaen_HK
dc.subjectPreparative chromatographyen_HK
dc.subjectZeaxanthinen_HK
dc.titleIsolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatographyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1064&spage=183&epage=186&date=2005&atitle=Isolation+and+purification+of+the+bioactive+carotenoid+zeaxanthin+from+the+microalga+Microcystis+aeruginosa+by+high-speed+counter-current+chromatographyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2004.12.065en_HK
dc.identifier.pmid15739885en_HK
dc.identifier.scopuseid_2-s2.0-12344335183en_HK
dc.identifier.hkuros104941en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-12344335183&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1064en_HK
dc.identifier.issue2en_HK
dc.identifier.spage183en_HK
dc.identifier.epage186en_HK
dc.identifier.isiWOS:000226782100005-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridWong, RNS=7402126957en_HK
dc.identifier.scopusauthoridJi, B=36642013700en_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK

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