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Article: Purification of trans-astaxanthin from a high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis

TitlePurification of trans-astaxanthin from a high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis
Authors
Issue Date2000
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/foodchem
Citation
Food Chemistry, 2000, v. 68 n. 4, p. 443-448 How to Cite?
AbstractThe purification method including extraction, saponification, and separation was established for preparing free trans-astaxanthin from a high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis which contained 3.67% trans-astaxanthins and 1.35% cis-astaxanthins of the dry cells. Low temperature (5°C) was chosen to minimize the degradation of astaxanthins during saponification, and 94.4% free trans-astaxanthin was obtained from trans-astaxanthin esters after 12 h of saponification. With this method, 32.2 mg trans-astaxanthin was obtained from 1 g dry algal cells. In addition, a new gradient reversed-phase HPLC method, suited for the quick analysis of free astaxanthins and astaxanthin esters in the unsaponified and saponified pigment extracts from the high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis, was developed and applied to the determination of astaxanthin contents during the processes of extraction, saponification, and purification. Copyright (C) 2000 Elsevier Science Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/68676
ISSN
2015 Impact Factor: 4.052
2015 SCImago Journal Rankings: 1.620
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYuan, JPen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:06:40Z-
dc.date.available2010-09-06T06:06:40Z-
dc.date.issued2000en_HK
dc.identifier.citationFood Chemistry, 2000, v. 68 n. 4, p. 443-448en_HK
dc.identifier.issn0308-8146en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68676-
dc.description.abstractThe purification method including extraction, saponification, and separation was established for preparing free trans-astaxanthin from a high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis which contained 3.67% trans-astaxanthins and 1.35% cis-astaxanthins of the dry cells. Low temperature (5°C) was chosen to minimize the degradation of astaxanthins during saponification, and 94.4% free trans-astaxanthin was obtained from trans-astaxanthin esters after 12 h of saponification. With this method, 32.2 mg trans-astaxanthin was obtained from 1 g dry algal cells. In addition, a new gradient reversed-phase HPLC method, suited for the quick analysis of free astaxanthins and astaxanthin esters in the unsaponified and saponified pigment extracts from the high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis, was developed and applied to the determination of astaxanthin contents during the processes of extraction, saponification, and purification. Copyright (C) 2000 Elsevier Science Ltd.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/foodchemen_HK
dc.relation.ispartofFood Chemistryen_HK
dc.rightsFood Chemistry. Copyright © Elsevier BV.en_HK
dc.titlePurification of trans-astaxanthin from a high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0308-8146&volume=68&spage=443&epage=448&date=2000&atitle=Purification+of+trans-astaxanthin+from+a+high-yielding+astaxanthin+ester-producing+strain+of+the+microalga+Haematococcus+pluvialisen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0308-8146(99)00219-8en_HK
dc.identifier.scopuseid_2-s2.0-0033990687en_HK
dc.identifier.hkuros52899en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033990687&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume68en_HK
dc.identifier.issue4en_HK
dc.identifier.spage443en_HK
dc.identifier.epage448en_HK
dc.identifier.isiWOS:000084848400010-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridYuan, JP=7403401467en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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