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Article: Expression of three members of the calcium-dependent protein kinase gene family in Arabidopsis thaliana

TitleExpression of three members of the calcium-dependent protein kinase gene family in Arabidopsis thaliana
Authors
KeywordsArabidopsis thaliana
calcium-dependent protein kinase
gene structure
immunostaining
Issue Date1996
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412
Citation
Plant Molecular Biology, 1996, v. 30 n. 6, p. 1259-1275 How to Cite?
AbstractCalcium-dependent protein kinases (CDPKs) belong to a unique family of enzymes containing a single polypeptide chain with a kinase domain at the amino terminus and a putative calcium-binding EF hands structure at the carboxyl terminus. From Arabidopsis thaliana, we have cloned three distinct cDNA sequences encoding CDPKs, which were designated as atcdpk6, atcdpk9 and atcdpk19. The full-length cDNA sequences for atcdpk6, atcdpk9 and atcdpk19 encode proteins with a molecular weight of 59343, 55376 and 59947, respectively. Recombinant atCDPK6 and atCDPK9 proteins were fully active as kinases whose activities were induced by Ca 2+. Biochemical studies suggested the presence of an autoinhibitory domain in the junction between the kinase domain and the EF hands structure. Serial deletion of the four EF hands of atCDPK6 demonstrated that the integrity of the four EF hands was crucial to the Ca 2+ response. All the three atcdpk genes were ubiquitously expressed in the plant as demonstrated by RNA gel blot experiments. Comparison of the genomic sequences suggested that the three cdpk genes have evolved differently. Using antibodies against atCDPK6 and atCDPK9 for immunohistochemical experiments, CDPKs were found to be expressed in specific cell types in a temporally and developmentally regulated manner.
Persistent Identifierhttp://hdl.handle.net/10722/68674
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 1.915
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHong, Yen_HK
dc.contributor.authorTakano, Men_HK
dc.contributor.authorLiu, CMen_HK
dc.contributor.authorGasch, Aen_HK
dc.contributor.authorChye, MLen_HK
dc.contributor.authorChua, NHen_HK
dc.date.accessioned2010-09-06T06:06:39Z-
dc.date.available2010-09-06T06:06:39Z-
dc.date.issued1996en_HK
dc.identifier.citationPlant Molecular Biology, 1996, v. 30 n. 6, p. 1259-1275en_HK
dc.identifier.issn0167-4412en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68674-
dc.description.abstractCalcium-dependent protein kinases (CDPKs) belong to a unique family of enzymes containing a single polypeptide chain with a kinase domain at the amino terminus and a putative calcium-binding EF hands structure at the carboxyl terminus. From Arabidopsis thaliana, we have cloned three distinct cDNA sequences encoding CDPKs, which were designated as atcdpk6, atcdpk9 and atcdpk19. The full-length cDNA sequences for atcdpk6, atcdpk9 and atcdpk19 encode proteins with a molecular weight of 59343, 55376 and 59947, respectively. Recombinant atCDPK6 and atCDPK9 proteins were fully active as kinases whose activities were induced by Ca 2+. Biochemical studies suggested the presence of an autoinhibitory domain in the junction between the kinase domain and the EF hands structure. Serial deletion of the four EF hands of atCDPK6 demonstrated that the integrity of the four EF hands was crucial to the Ca 2+ response. All the three atcdpk genes were ubiquitously expressed in the plant as demonstrated by RNA gel blot experiments. Comparison of the genomic sequences suggested that the three cdpk genes have evolved differently. Using antibodies against atCDPK6 and atCDPK9 for immunohistochemical experiments, CDPKs were found to be expressed in specific cell types in a temporally and developmentally regulated manner.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412en_HK
dc.relation.ispartofPlant Molecular Biologyen_HK
dc.subjectArabidopsis thalianaen_HK
dc.subjectcalcium-dependent protein kinaseen_HK
dc.subjectgene structureen_HK
dc.subjectimmunostainingen_HK
dc.titleExpression of three members of the calcium-dependent protein kinase gene family in Arabidopsis thalianaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-4412&volume=30&spage=1259&epage=1275&date=1996&atitle=Expression+of+three+members+of+the+calcium-dependent+protein+kinase+gene+family+in+Arabidopsis+thalianaen_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8704134-
dc.identifier.scopuseid_2-s2.0-0030110747en_HK
dc.identifier.hkuros13092en_HK
dc.identifier.volume30en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1259en_HK
dc.identifier.epage1275en_HK
dc.identifier.isiWOS:A1996UX50400015-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHong, Y=36082936200en_HK
dc.identifier.scopusauthoridTakano, M=35273558100en_HK
dc.identifier.scopusauthoridLiu, CM=7409786807en_HK
dc.identifier.scopusauthoridGasch, A=23469038400en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK
dc.identifier.scopusauthoridChua, NH=36863130400en_HK

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