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Article: Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection

TitleSimultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection
Authors
KeywordsColumn liquid chromatography
Fluorescence detection
Pharmaceuticals
Tetracycline antibiotics
Issue Date2004
PublisherFriedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://www.elsevier.com/locate/chromatographia
Citation
Chromatographia, 2004, v. 60 n. 5-6, p. 259-264 How to Cite?
AbstractA simple, rapid and sensitive high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline was developed, and successfully applied to the analysis of commercial tetracycline antibiotics. The separation was performed on a reverse-phase C18 column with a gradient elution composed of methanol and sodium acetate buffer (containing disodium ethylenediaminetetraacetate and calcium chloride, pH 8.10) as the mobile phase, and fluorescence detection at 532 nm (excitation at 380 nm). The detection limits for oxytetracycline, doxycycline, tetracycline and chlortetracycline were 0.1, 0.5, 0.3 and 0.4 μg L-1, respectively. Data with respect to precision and accuracy were reported and discussed.
Persistent Identifierhttp://hdl.handle.net/10722/68671
ISSN
2015 Impact Factor: 1.332
2015 SCImago Journal Rankings: 0.480
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, HTen_HK
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorLi, HBen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorWong, MHen_HK
dc.date.accessioned2010-09-06T06:06:38Z-
dc.date.available2010-09-06T06:06:38Z-
dc.date.issued2004en_HK
dc.identifier.citationChromatographia, 2004, v. 60 n. 5-6, p. 259-264en_HK
dc.identifier.issn0009-5893en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68671-
dc.description.abstractA simple, rapid and sensitive high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline was developed, and successfully applied to the analysis of commercial tetracycline antibiotics. The separation was performed on a reverse-phase C18 column with a gradient elution composed of methanol and sodium acetate buffer (containing disodium ethylenediaminetetraacetate and calcium chloride, pH 8.10) as the mobile phase, and fluorescence detection at 532 nm (excitation at 380 nm). The detection limits for oxytetracycline, doxycycline, tetracycline and chlortetracycline were 0.1, 0.5, 0.3 and 0.4 μg L-1, respectively. Data with respect to precision and accuracy were reported and discussed.en_HK
dc.languageengen_HK
dc.publisherFriedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://www.elsevier.com/locate/chromatographiaen_HK
dc.relation.ispartofChromatographiaen_HK
dc.subjectColumn liquid chromatographyen_HK
dc.subjectFluorescence detectionen_HK
dc.subjectPharmaceuticalsen_HK
dc.subjectTetracycline antibioticsen_HK
dc.titleSimultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0009-5893&volume=60&spage=259&epage=264&date=2004&atitle=Simultaneous+determination+of+oxytetracycline,+doxycycline,+tetracycline+and+chlortetracycline+in+tetracycline+antibiotics+by+high-performance+liquid+chromatography+with+fluorescence+detectionen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1365/s10337-004-0356-zen_HK
dc.identifier.scopuseid_2-s2.0-4744362375en_HK
dc.identifier.hkuros104991en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4744362375&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_HK
dc.identifier.issue5-6en_HK
dc.identifier.spage259en_HK
dc.identifier.epage264en_HK
dc.identifier.isiWOS:000224101000002-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLu, HT=7404843255en_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridWong, MH=7403908633en_HK

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