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Article: Simultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography with diode array detection

TitleSimultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography with diode array detection
Authors
KeywordsDrugs
High performance liquid chromatography
Vitamins
Issue Date2001
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jss
Citation
Journal Of Separation Science, 2001, v. 24 n. 4, p. 271-274 How to Cite?
AbstractA simple and rapid method for the simultaneous determination of nine water-soluble vitamins was developed by high performance liquid chromatography with diode array detection. The water-soluble vitamins were analyzed by HPLC on a μBondapak C18 column (300 × 3.9 mm, 10 μm) with methanol-0.1 mol/L KH2PO4 buffer (pH 7.0) as mobile phase (1.5 mL/min) in gradient mode as follows: in the range 0-7.0 min, 10:90; and in the range 8.0-15.0 min, 40:60 (v/v). All nine water-soluble vitamins were separated in a single HPLC run within less than 15 min. The linearity of the calibration graphs was compound-dependent with correlation coefficients of 0.9986 to 0.9999 (n = 6). The detection limits ranged from 0.02 μg/mL to 0.5 μg/mL. The method was successfully applied to the determination of vitamins in pharmaceutical preparations. The recoveries were from 95% to 102% and the relative standard deviations were in the range of 0.8% to 4.2%.
Persistent Identifierhttp://hdl.handle.net/10722/68670
ISSN
2015 Impact Factor: 2.741
2015 SCImago Journal Rankings: 1.041
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:06:37Z-
dc.date.available2010-09-06T06:06:37Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Separation Science, 2001, v. 24 n. 4, p. 271-274en_HK
dc.identifier.issn1615-9306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68670-
dc.description.abstractA simple and rapid method for the simultaneous determination of nine water-soluble vitamins was developed by high performance liquid chromatography with diode array detection. The water-soluble vitamins were analyzed by HPLC on a μBondapak C18 column (300 × 3.9 mm, 10 μm) with methanol-0.1 mol/L KH2PO4 buffer (pH 7.0) as mobile phase (1.5 mL/min) in gradient mode as follows: in the range 0-7.0 min, 10:90; and in the range 8.0-15.0 min, 40:60 (v/v). All nine water-soluble vitamins were separated in a single HPLC run within less than 15 min. The linearity of the calibration graphs was compound-dependent with correlation coefficients of 0.9986 to 0.9999 (n = 6). The detection limits ranged from 0.02 μg/mL to 0.5 μg/mL. The method was successfully applied to the determination of vitamins in pharmaceutical preparations. The recoveries were from 95% to 102% and the relative standard deviations were in the range of 0.8% to 4.2%.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jssen_HK
dc.relation.ispartofJournal of Separation Scienceen_HK
dc.subjectDrugsen_HK
dc.subjectHigh performance liquid chromatographyen_HK
dc.subjectVitaminsen_HK
dc.titleSimultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography with diode array detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9306&volume=24&spage=271&epage=274&date=2001&atitle=Simultaneous+determination+of+nine+water-soluble+vitamins+in+pharmaceutical+preparations+by+high-performance+liquid+chromatography+with+diode+array+detectionen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1615-9314(20010401)24:4<271::AID-JSSC271>3.0.CO;2-Len_HK
dc.identifier.scopuseid_2-s2.0-0035020502en_HK
dc.identifier.hkuros61475en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035020502&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue4en_HK
dc.identifier.spage271en_HK
dc.identifier.epage274en_HK
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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