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Article: Isolation of a gene encoding Arabidopsis membrane-associated acyl-CoA binding protein and immunolocalization of its gene product

TitleIsolation of a gene encoding Arabidopsis membrane-associated acyl-CoA binding protein and immunolocalization of its gene product
Authors
Issue Date1999
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412
Citation
Plant Journal, 1999, v. 18 n. 2, p. 205-214 How to Cite?
AbstractUntil recently, only cytosolic acyl-CoA binding proteins (ACBPs) have been characterized. The isolation of an Arabidopsis thaliana cDNA encoding a novel membrane-associated ACBP that accumulates in developing seeds, designated ACBP1, has provided evidence for the existence of membrane-associated forms of ACBPs (Chye, 1998, Plant Mol. Biol. 38, 827-838). We now report on the isolation of its corresponding gene from an A. thaliana Columbia genomic library using the ACBP1 cDNA as a hybridization probe. Nucleotide sequence analysis of Arabidopsis ACBP1 showed that its promoter lacks a TATA box, resembling the promoters of rat, Drosophila and human genes encoding cytosolic ACBP and suggesting that it is a housekeeping gene. We show by Western blot analysis that ACBP1 expression in developing seeds coincides with lipid deposition and that homologues of membrane-associated ACBP1 exist in other plants. Using light microscopy, we show that ACBP1 is strongly expressed in the embryo at the cotyledons, hypocotyl, procambium of the axis and in most peripheral cells of the cotyledons and hypocotyl, immunogold labelling localized ACBP1 to vesicles, to the plasma membrane especially at epidermal cells of heart, torpedo and cotyledonary stage embryos, and to the cell wall of the outer integument cells at the seed coat. Our results suggest that ACBP1 is involved in intermembrane lipid transport from the ER via vesicles to the plasma membrane where it could maintain a membrane-associated acyl pool; its immunolocalization to the cell wall of outer integument cells at the seed coat suggests a role in cuticle and cutin formation.
Persistent Identifierhttp://hdl.handle.net/10722/68657
ISSN
2015 Impact Factor: 5.468
2015 SCImago Journal Rankings: 3.457
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChye, MLen_HK
dc.contributor.authorHuang, BQen_HK
dc.contributor.authorZee, SYen_HK
dc.date.accessioned2010-09-06T06:06:31Z-
dc.date.available2010-09-06T06:06:31Z-
dc.date.issued1999en_HK
dc.identifier.citationPlant Journal, 1999, v. 18 n. 2, p. 205-214en_HK
dc.identifier.issn0960-7412en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68657-
dc.description.abstractUntil recently, only cytosolic acyl-CoA binding proteins (ACBPs) have been characterized. The isolation of an Arabidopsis thaliana cDNA encoding a novel membrane-associated ACBP that accumulates in developing seeds, designated ACBP1, has provided evidence for the existence of membrane-associated forms of ACBPs (Chye, 1998, Plant Mol. Biol. 38, 827-838). We now report on the isolation of its corresponding gene from an A. thaliana Columbia genomic library using the ACBP1 cDNA as a hybridization probe. Nucleotide sequence analysis of Arabidopsis ACBP1 showed that its promoter lacks a TATA box, resembling the promoters of rat, Drosophila and human genes encoding cytosolic ACBP and suggesting that it is a housekeeping gene. We show by Western blot analysis that ACBP1 expression in developing seeds coincides with lipid deposition and that homologues of membrane-associated ACBP1 exist in other plants. Using light microscopy, we show that ACBP1 is strongly expressed in the embryo at the cotyledons, hypocotyl, procambium of the axis and in most peripheral cells of the cotyledons and hypocotyl, immunogold labelling localized ACBP1 to vesicles, to the plasma membrane especially at epidermal cells of heart, torpedo and cotyledonary stage embryos, and to the cell wall of the outer integument cells at the seed coat. Our results suggest that ACBP1 is involved in intermembrane lipid transport from the ER via vesicles to the plasma membrane where it could maintain a membrane-associated acyl pool; its immunolocalization to the cell wall of outer integument cells at the seed coat suggests a role in cuticle and cutin formation.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412en_HK
dc.relation.ispartofPlant Journalen_HK
dc.rightsThe Plant Journal. Copyright © Blackwell Publishing Ltd.en_HK
dc.titleIsolation of a gene encoding Arabidopsis membrane-associated acyl-CoA binding protein and immunolocalization of its gene producten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0960-7412&volume=18&spage=205&epage=214&date=1999&atitle=Isolation+of+a+gene+encoding+Arabidopsis+membrane-associated+acyl-CoA+binding+protein+and+immunolocalization+of+its+gene+producten_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-313X.1999.00443.xen_HK
dc.identifier.pmid10363372-
dc.identifier.scopuseid_2-s2.0-0033118479en_HK
dc.identifier.hkuros46518en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033118479&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue2en_HK
dc.identifier.spage205en_HK
dc.identifier.epage214en_HK
dc.identifier.isiWOS:000080474500009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK
dc.identifier.scopusauthoridHuang, BQ=13607908600en_HK
dc.identifier.scopusauthoridZee, SY=7004001733en_HK

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