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Article: Effects of environmental factors and denaturants on the specific absorption spectrum and fluorescence characters of B-phycoerythrin

TitleEffects of environmental factors and denaturants on the specific absorption spectrum and fluorescence characters of B-phycoerythrin
Authors
KeywordsDenaturant
Environmental factors
Fluorescence
Phycoerythrin
Specific absorption spectrum
Issue Date2006
Citation
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy And Spectral Analysis, 2006, v. 26 n. 12, p. 2258-2263 How to Cite?
AbstractEffects of environmental factors and denaturants on the variation rules of the specific absorption spectrum and fluorescence emission intensity of B-phycoerythrin were systematically investigated in the present paper. The results showed that B-phycoerythrin was relatively stable under certain conditions: the pH range from 4. 5 to 9. 5, temperature range from 25 to 60°C, and illumination intensity at 2 800 lx in 24 h. The specific absorption spectrum and fluorescence emission intensity of B-phycoerythrin changed slightly. The conformation of Br phycoerythrin changed remarkably with 0. 5% -3% SDS or 0. 25%-l% β-mercaptoethanol, the fluorescence emission intensity declined greatly, and the specific absorption peaks disappeared. The fluorescence emission intensity declined, while the specific absorption spectrum did not change, but the peak intensity reduced slightly with 10%-70% acetonitrile or 2-8 mol · L -1 urea. The possible reasons for the conformation changes of B-phycoerythrin with various environmental factors and different denaturants were briefly discussed.
Persistent Identifierhttp://hdl.handle.net/10722/68654
ISSN
2015 Impact Factor: 0.275
2015 SCImago Journal Rankings: 0.199

 

DC FieldValueLanguage
dc.contributor.authorHu, JMen_HK
dc.contributor.authorWei, Den_HK
dc.contributor.authorGuo, SYen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:06:29Z-
dc.date.available2010-09-06T06:06:29Z-
dc.date.issued2006en_HK
dc.identifier.citationGuang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy And Spectral Analysis, 2006, v. 26 n. 12, p. 2258-2263en_HK
dc.identifier.issn1000-0593en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68654-
dc.description.abstractEffects of environmental factors and denaturants on the variation rules of the specific absorption spectrum and fluorescence emission intensity of B-phycoerythrin were systematically investigated in the present paper. The results showed that B-phycoerythrin was relatively stable under certain conditions: the pH range from 4. 5 to 9. 5, temperature range from 25 to 60°C, and illumination intensity at 2 800 lx in 24 h. The specific absorption spectrum and fluorescence emission intensity of B-phycoerythrin changed slightly. The conformation of Br phycoerythrin changed remarkably with 0. 5% -3% SDS or 0. 25%-l% β-mercaptoethanol, the fluorescence emission intensity declined greatly, and the specific absorption peaks disappeared. The fluorescence emission intensity declined, while the specific absorption spectrum did not change, but the peak intensity reduced slightly with 10%-70% acetonitrile or 2-8 mol · L -1 urea. The possible reasons for the conformation changes of B-phycoerythrin with various environmental factors and different denaturants were briefly discussed.en_HK
dc.languageengen_HK
dc.relation.ispartofGuang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysisen_HK
dc.subjectDenaturanten_HK
dc.subjectEnvironmental factorsen_HK
dc.subjectFluorescenceen_HK
dc.subjectPhycoerythrinen_HK
dc.subjectSpecific absorption spectrumen_HK
dc.titleEffects of environmental factors and denaturants on the specific absorption spectrum and fluorescence characters of B-phycoerythrinen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-33847267678en_HK
dc.identifier.hkuros137105en_HK
dc.identifier.volume26en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2258en_HK
dc.identifier.epage2263en_HK
dc.identifier.scopusauthoridHu, JM=16021789200en_HK
dc.identifier.scopusauthoridWei, D=24472144100en_HK
dc.identifier.scopusauthoridGuo, SY=8514940700en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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