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Article: Chromatin structure modulation in Saccharomyces cerevisiae by centromere and promoter factor 1

TitleChromatin structure modulation in Saccharomyces cerevisiae by centromere and promoter factor 1
Authors
Issue Date1994
PublisherAmerican Society for Microbiology.
Citation
Molecular And Cellular Biology, 1994, v. 14 n. 8, p. 5229-5241 How to Cite?
AbstractCPF1 is an abundant basic-helix-loop-helix-ZIP protein that binds to the CDEI motif in Saccharomyces cerevisiae centromeres and in the promoters of numerous genes, including those encoding enzymes of the methionine biosynthetic pathway. Strains lacking CPF1 are methionine auxotrophs, and it has been proposed that CPF1 might positively influence transcription at the MET25 and MET16 genes by modulating promoter chromatin structure. We test this hypothesis and show that the regions surrounding the CDEI motifs in the MET25 and MET16 promoters are maintained in a nucleosome-free state and that this requires the entire CPF1 protein. However, the chromatin structure around the CDEI motifs does not change on derepression of transcription and does not correlate with the methionine phenotype of the cell. An intact CDEI motif but not CPF1 is required for transcriptional activation from a region of the MET25 upstream activation sequence. Our results suggest that CPF1 functions to modulate chromatin structure around the CDEI motif but that these changes at the MET25 and MET16 promoters do not explain how CPF1 functions to maintain methionine-independent growth. The presence of CPF1- dependent chromatin structures at these promoters leads to a weak repression of transcription.
Persistent Identifierhttp://hdl.handle.net/10722/68649
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
PubMed Central ID
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DC FieldValueLanguage
dc.contributor.authorKent, NAen_HK
dc.contributor.authorTsang, JSHen_HK
dc.contributor.authorCrowther, DJen_HK
dc.contributor.authorMellor, Jen_HK
dc.date.accessioned2010-09-06T06:06:27Z-
dc.date.available2010-09-06T06:06:27Z-
dc.date.issued1994en_HK
dc.identifier.citationMolecular And Cellular Biology, 1994, v. 14 n. 8, p. 5229-5241en_HK
dc.identifier.issn0270-7306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68649-
dc.description.abstractCPF1 is an abundant basic-helix-loop-helix-ZIP protein that binds to the CDEI motif in Saccharomyces cerevisiae centromeres and in the promoters of numerous genes, including those encoding enzymes of the methionine biosynthetic pathway. Strains lacking CPF1 are methionine auxotrophs, and it has been proposed that CPF1 might positively influence transcription at the MET25 and MET16 genes by modulating promoter chromatin structure. We test this hypothesis and show that the regions surrounding the CDEI motifs in the MET25 and MET16 promoters are maintained in a nucleosome-free state and that this requires the entire CPF1 protein. However, the chromatin structure around the CDEI motifs does not change on derepression of transcription and does not correlate with the methionine phenotype of the cell. An intact CDEI motif but not CPF1 is required for transcriptional activation from a region of the MET25 upstream activation sequence. Our results suggest that CPF1 functions to modulate chromatin structure around the CDEI motif but that these changes at the MET25 and MET16 promoters do not explain how CPF1 functions to maintain methionine-independent growth. The presence of CPF1- dependent chromatin structures at these promoters leads to a weak repression of transcription.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofMolecular and Cellular Biologyen_HK
dc.rightsMolecular and Cellular Biology. Copyright © American Society for Microbiology.en_HK
dc.titleChromatin structure modulation in Saccharomyces cerevisiae by centromere and promoter factor 1en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-7306&volume=14&spage=5229&epage=5241&date=1994&atitle=Chromatin+structure+modulation+in+Saccharomyces+cerevisiae+by+centromere+and+promoter+factor+1en_HK
dc.identifier.emailTsang, JSH: jshtsang@hku.hken_HK
dc.identifier.authorityTsang, JSH=rp00792en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid8035802-
dc.identifier.pmcidPMC359042-
dc.identifier.scopuseid_2-s2.0-0028017601en_HK
dc.identifier.hkuros479en_HK
dc.identifier.volume14en_HK
dc.identifier.issue8en_HK
dc.identifier.spage5229en_HK
dc.identifier.epage5241en_HK
dc.identifier.isiWOS:A1994NY42900022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKent, NA=7003693811en_HK
dc.identifier.scopusauthoridTsang, JSH=7102483508en_HK
dc.identifier.scopusauthoridCrowther, DJ=7103297153en_HK
dc.identifier.scopusauthoridMellor, J=7103106833en_HK

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