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Article: AFLP and RFLP linkage map in Coix
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TitleAFLP and RFLP linkage map in Coix
 
AuthorsQin, F2
Li, J3
Li, X2
Corke, H1
 
KeywordsAFLP
Coix
Genetic map
RFLP
 
Issue Date2005
 
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0925-9864
 
CitationGenetic Resources And Crop Evolution, 2005, v. 52 n. 2, p. 209-214 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10722-003-1387-2
 
AbstractCoix is a genus in the grass family placed in the tribe Maydeae. It is closely related to maize and is also used as a crop plant. Since many valuable traits have been identified recently in Coix, it is considered to be a valuable genetic resource, particularly for maize improvement. In this study, a Coix genetic linkage map was constructed using an F2 population of 131 individuals. Eighty AFLP and 10 RFLP markers were mapped, covering a total length of 1339.5 cM with an average interval of 14.88 cM. The map consisted of 10 linkage groups, were consistent with the chromosome numbers observed cytogenetically. Both AFLP and RFLP markers were used first for genetic analysis in Coix. AFLP markers were generated by two restriction enzyme combinations, EcoRI/MseI and PstI/MseI. A total of 1349 bands were amplified, of which 140 were polymorphic. The polymorphism detection efficiency of the two enzyme combinations was compared, and utility of AFLP markers to construct the linkage map was discussed. Ten RFLP markers detected by three different probes were distributed on eight different linkage groups. The results provide a foundation to map and isolate important genes in Coix, and to investigate its genomic architecture, possible origins, and relationship with maize at the DNA level. © Springer 2005.
 
ISSN0925-9864
2013 Impact Factor: 1.482
 
DOIhttp://dx.doi.org/10.1007/s10722-003-1387-2
 
ISI Accession Number IDWOS:000228500300012
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorQin, F
 
dc.contributor.authorLi, J
 
dc.contributor.authorLi, X
 
dc.contributor.authorCorke, H
 
dc.date.accessioned2010-09-06T06:06:24Z
 
dc.date.available2010-09-06T06:06:24Z
 
dc.date.issued2005
 
dc.description.abstractCoix is a genus in the grass family placed in the tribe Maydeae. It is closely related to maize and is also used as a crop plant. Since many valuable traits have been identified recently in Coix, it is considered to be a valuable genetic resource, particularly for maize improvement. In this study, a Coix genetic linkage map was constructed using an F2 population of 131 individuals. Eighty AFLP and 10 RFLP markers were mapped, covering a total length of 1339.5 cM with an average interval of 14.88 cM. The map consisted of 10 linkage groups, were consistent with the chromosome numbers observed cytogenetically. Both AFLP and RFLP markers were used first for genetic analysis in Coix. AFLP markers were generated by two restriction enzyme combinations, EcoRI/MseI and PstI/MseI. A total of 1349 bands were amplified, of which 140 were polymorphic. The polymorphism detection efficiency of the two enzyme combinations was compared, and utility of AFLP markers to construct the linkage map was discussed. Ten RFLP markers detected by three different probes were distributed on eight different linkage groups. The results provide a foundation to map and isolate important genes in Coix, and to investigate its genomic architecture, possible origins, and relationship with maize at the DNA level. © Springer 2005.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationGenetic Resources And Crop Evolution, 2005, v. 52 n. 2, p. 209-214 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10722-003-1387-2
 
dc.identifier.citeulike164765
 
dc.identifier.doihttp://dx.doi.org/10.1007/s10722-003-1387-2
 
dc.identifier.epage214
 
dc.identifier.hkuros106373
 
dc.identifier.isiWOS:000228500300012
 
dc.identifier.issn0925-9864
2013 Impact Factor: 1.482
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-17744384775
 
dc.identifier.spage209
 
dc.identifier.urihttp://hdl.handle.net/10722/68644
 
dc.identifier.volume52
 
dc.languageeng
 
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0925-9864
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofGenetic Resources and Crop Evolution
 
dc.relation.referencesReferences in Scopus
 
dc.subjectAFLP
 
dc.subjectCoix
 
dc.subjectGenetic map
 
dc.subjectRFLP
 
dc.titleAFLP and RFLP linkage map in Coix
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Huazhong Agricultural University
  3. China Agricultural University