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Article: Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions

TitleMixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions
Authors
KeywordsCell suspensions
Growth
Haematococcus lacustris
Heterotrophic
Mixotrophic
Theology
Viscosity
Issue Date1997
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biortech
Citation
Bioresource Technology, 1997, v. 62 n. 1-2, p. 19-24 How to Cite?
AbstractMixotrophic and heterotrophic growth of Haematococcus lacustris and theological behaviour of the cell suspensions were investigated using batch culture in a 3.7 l fermenter. The microalga grew well on acetate in both growth modes, although growth was much better in mixotrophic culture than in the heterotrophic culture. The apparent viscosity of the cell-free medium of the two cultures was about 1 cp indicating that no viscous extracellular products such as polysaccharides were produced. In contrast, the apparent viscosity of the cell suspension increased with increasing cell concentrations. The viscosity was affected not only by the cell density but also the growth mode. In heterotrophic culture, the apparent viscosity increased steeply with cell concentrations above approx. 6.5 g l-1. In mixotrophic culture, however, the apparent viscosity increased markedly only when cell concentrations were higher than 25 g l-1. For both growth conditions, at low concentrations (i.e. < approx. 10 g l-1, the cell suspensions exhibited Newtonian characteristics, whereas at higher concentrations (i.e. > approx. 10 g l-1, the cell suspensions became non-Newtonian. The non-Newtonian characteristics of the culture fluids could be described by the Bingham plastic model. Both the yield stress and plastic viscosity increased with increasing cell concentrations. | Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions were investigated using batch culture in a 3.7 l fermenter. The microalga grew well on acetate in both growth modes, although growth was much better in mixotrophic culture than in the heterotrophic culture. The apparent viscosity of the cell-free medium of the two cultures was about 1 cp indicating that no viscous extracellular products such as polysaccharides were produced. In contrast, the apparent viscosity of the cell suspensions increased with increasing cell concentrations. The viscosity was affected not only by the cell density but also by the growth mode. In heterotrophic culture, the apparent viscosity increased steeply with cell concentrations above approx. 6.5 g l-1. In mixotrophic culture, however, the apparent viscosity increased markedly only when cell concentrations were higher than 25 g l-1. For both growth conditions, at low concentrations (i.e. approx. 10 g l-1), the cell suspensions became non-Newtonian. The non-Newtonian characteristics of the culture fluids could be described by the Bingham plastic model. Both the yield stress and plastic viscosity increased with increasing cell concentrations.
Persistent Identifierhttp://hdl.handle.net/10722/68628
ISSN
2015 Impact Factor: 4.917
2015 SCImago Journal Rankings: 2.303
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Fen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorGong, Xen_HK
dc.date.accessioned2010-09-06T06:06:16Z-
dc.date.available2010-09-06T06:06:16Z-
dc.date.issued1997en_HK
dc.identifier.citationBioresource Technology, 1997, v. 62 n. 1-2, p. 19-24en_HK
dc.identifier.issn0960-8524en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68628-
dc.description.abstractMixotrophic and heterotrophic growth of Haematococcus lacustris and theological behaviour of the cell suspensions were investigated using batch culture in a 3.7 l fermenter. The microalga grew well on acetate in both growth modes, although growth was much better in mixotrophic culture than in the heterotrophic culture. The apparent viscosity of the cell-free medium of the two cultures was about 1 cp indicating that no viscous extracellular products such as polysaccharides were produced. In contrast, the apparent viscosity of the cell suspension increased with increasing cell concentrations. The viscosity was affected not only by the cell density but also the growth mode. In heterotrophic culture, the apparent viscosity increased steeply with cell concentrations above approx. 6.5 g l-1. In mixotrophic culture, however, the apparent viscosity increased markedly only when cell concentrations were higher than 25 g l-1. For both growth conditions, at low concentrations (i.e. < approx. 10 g l-1, the cell suspensions exhibited Newtonian characteristics, whereas at higher concentrations (i.e. > approx. 10 g l-1, the cell suspensions became non-Newtonian. The non-Newtonian characteristics of the culture fluids could be described by the Bingham plastic model. Both the yield stress and plastic viscosity increased with increasing cell concentrations. | Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions were investigated using batch culture in a 3.7 l fermenter. The microalga grew well on acetate in both growth modes, although growth was much better in mixotrophic culture than in the heterotrophic culture. The apparent viscosity of the cell-free medium of the two cultures was about 1 cp indicating that no viscous extracellular products such as polysaccharides were produced. In contrast, the apparent viscosity of the cell suspensions increased with increasing cell concentrations. The viscosity was affected not only by the cell density but also by the growth mode. In heterotrophic culture, the apparent viscosity increased steeply with cell concentrations above approx. 6.5 g l-1. In mixotrophic culture, however, the apparent viscosity increased markedly only when cell concentrations were higher than 25 g l-1. For both growth conditions, at low concentrations (i.e. <approx. 10 g l-1), the cell suspensions exhibited Newtonian characteristics, whereas at higher concentrations (i.e. > approx. 10 g l-1), the cell suspensions became non-Newtonian. The non-Newtonian characteristics of the culture fluids could be described by the Bingham plastic model. Both the yield stress and plastic viscosity increased with increasing cell concentrations.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biortechen_HK
dc.relation.ispartofBioresource Technologyen_HK
dc.rightsBioresource Technology. Copyright © Elsevier BV.en_HK
dc.subjectCell suspensionsen_HK
dc.subjectGrowthen_HK
dc.subjectHaematococcus lacustrisen_HK
dc.subjectHeterotrophicen_HK
dc.subjectMixotrophicen_HK
dc.subjectTheologyen_HK
dc.subjectViscosityen_HK
dc.titleMixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0960-8524&volume=62&issue=1-2&spage=19&epage=24&date=1997&atitle=Mixotrophic+and+heterotrophic+growth+of+Haematococcus+lacustris+and+rheological+behaviour+of+the+cell+suspensionsen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0960-8524(97)00115-6en_HK
dc.identifier.scopuseid_2-s2.0-0031253366en_HK
dc.identifier.hkuros35109en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031253366&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume62en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage19en_HK
dc.identifier.epage24en_HK
dc.identifier.isiWOS:000071379500003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridChen, H=26642984400en_HK
dc.identifier.scopusauthoridGong, X=7201999182en_HK

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