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Article: Heterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture

TitleHeterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture
Authors
Issue Date1996
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio
Citation
Process Biochemistry, 1996, v. 31 n. 6, p. 601-604 How to Cite?
AbstractThe possibility of using heterotrophic chemostat culture to achieve relatively high cell densities of Chlamydomonas reinhardtii by avoiding the inhibition of growth by the substrate, acetate was investigated. Continuous culture gave significant improvement in cell dry weight concentration and productivity compared to batch culture. It is postulated that this was due to low acetate concentration in the continuous culture. The highest cell concentration obtained was 1.5 g/litre at a feed acetate concentration of 3.4 g/litre. At acetate concentrations up to this level, the modified Monod model successfully predicted the steady-state cell concentration and cellular productivity. However, no further increase in cell concentration was achieved, despite the use of higher feed acetate concentrations (i.e. 5.1 g/litre), in which, steady-state acetate concentrations were below the inhibition level (i.e. 0.4 g/litre). This inhibitory effect was probably due to sodium rather than to acetate per se in the cultures.
Persistent Identifierhttp://hdl.handle.net/10722/68621
ISSN
2015 Impact Factor: 2.529
2015 SCImago Journal Rankings: 0.937
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Fen_HK
dc.contributor.authorJohns, MRen_HK
dc.date.accessioned2010-09-06T06:06:13Z-
dc.date.available2010-09-06T06:06:13Z-
dc.date.issued1996en_HK
dc.identifier.citationProcess Biochemistry, 1996, v. 31 n. 6, p. 601-604en_HK
dc.identifier.issn1359-5113en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68621-
dc.description.abstractThe possibility of using heterotrophic chemostat culture to achieve relatively high cell densities of Chlamydomonas reinhardtii by avoiding the inhibition of growth by the substrate, acetate was investigated. Continuous culture gave significant improvement in cell dry weight concentration and productivity compared to batch culture. It is postulated that this was due to low acetate concentration in the continuous culture. The highest cell concentration obtained was 1.5 g/litre at a feed acetate concentration of 3.4 g/litre. At acetate concentrations up to this level, the modified Monod model successfully predicted the steady-state cell concentration and cellular productivity. However, no further increase in cell concentration was achieved, despite the use of higher feed acetate concentrations (i.e. 5.1 g/litre), in which, steady-state acetate concentrations were below the inhibition level (i.e. 0.4 g/litre). This inhibitory effect was probably due to sodium rather than to acetate per se in the cultures.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbioen_HK
dc.relation.ispartofProcess Biochemistryen_HK
dc.rightsProcess Biochemistry. Copyright © Elsevier Ltd.en_HK
dc.titleHeterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat cultureen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1359-5113&volume=31&issue=6&spage=601&epage=604&date=1996&atitle=Heterotrophic+growth+of+Chlamydomonas+reinhardtii+on+acetate+in+chemostat+cultureen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0001164186en_HK
dc.identifier.hkuros12304en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0001164186&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue6en_HK
dc.identifier.spage601en_HK
dc.identifier.epage604en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridJohns, MR=7202660598en_HK

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