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Article: Proteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae)

TitleProteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae)
Authors
KeywordsAstaxanthin
Haematococcus
Oxidative stress
Protein expression
Proteomics
Stress response
Issue Date2004
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 2004, v. 220 n. 1, p. 17-29 How to Cite?
AbstractRapidly growing, green motile flagellates of Haematococcus pluvialis can transform into enlarged red resting cysts (aplanospores) under oxidative stress conditions. However, it is not known what initial molecular defense mechanisms occur in response to oxidative stress, and may ultimately lead to cellular transformation. In this study, global-expression profiling of cellular proteins in response to stress was analyzed by two-dimensional gel electrophoresis, image analysis, and peptide mass fingerprinting. Oxidative stress was induced in cultures of green flagellates by addition of acetate and Fe2+, and exposure to excess light intensity. Overall, 70 proteins were identified with altered expression patterns following stress induction. Some key proteins involved in photosynthesis and nitrogen assimilation were down-regulated, whereas some mitochondrial respiratory proteins were transiently up-regulated after the onset of stress. Most of the identified proteins, particularly those from the families of superoxide dismutase, catalase, and peroxidase, were transiently up-regulated, but reverted to down-regulation during the 6 days of stress. On the other hand, cellular accumulation of the antioxidant astaxanthin occurred well after initiation of oxidative stress and reached its maximum cellular level after six or more days of stress. It appears that the early stress response involves multiple enzymatic defense processes that play a critical role upon onset of stress and also during the early transition of green vegetative cells to red cysts. As cyst development continues, the intensive, enzyme-mediated initial responses were largely replaced in mature red cysts by accumulation of the molecular antioxidant astaxanthin. This study provides the first direct evidence for a massive, and concerted up-regulation of multiple antioxidative defense mechanisms, both spatially and temporarily, to protect H. pluvialis cells against oxidative stress. © Springer-Verlag 2004.
Persistent Identifierhttp://hdl.handle.net/10722/68607
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, SBen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorSommerfeld, Men_HK
dc.contributor.authorHu, Qen_HK
dc.date.accessioned2010-09-06T06:06:05Z-
dc.date.available2010-09-06T06:06:05Z-
dc.date.issued2004en_HK
dc.identifier.citationPlanta, 2004, v. 220 n. 1, p. 17-29en_HK
dc.identifier.issn0032-0935en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68607-
dc.description.abstractRapidly growing, green motile flagellates of Haematococcus pluvialis can transform into enlarged red resting cysts (aplanospores) under oxidative stress conditions. However, it is not known what initial molecular defense mechanisms occur in response to oxidative stress, and may ultimately lead to cellular transformation. In this study, global-expression profiling of cellular proteins in response to stress was analyzed by two-dimensional gel electrophoresis, image analysis, and peptide mass fingerprinting. Oxidative stress was induced in cultures of green flagellates by addition of acetate and Fe2+, and exposure to excess light intensity. Overall, 70 proteins were identified with altered expression patterns following stress induction. Some key proteins involved in photosynthesis and nitrogen assimilation were down-regulated, whereas some mitochondrial respiratory proteins were transiently up-regulated after the onset of stress. Most of the identified proteins, particularly those from the families of superoxide dismutase, catalase, and peroxidase, were transiently up-regulated, but reverted to down-regulation during the 6 days of stress. On the other hand, cellular accumulation of the antioxidant astaxanthin occurred well after initiation of oxidative stress and reached its maximum cellular level after six or more days of stress. It appears that the early stress response involves multiple enzymatic defense processes that play a critical role upon onset of stress and also during the early transition of green vegetative cells to red cysts. As cyst development continues, the intensive, enzyme-mediated initial responses were largely replaced in mature red cysts by accumulation of the molecular antioxidant astaxanthin. This study provides the first direct evidence for a massive, and concerted up-regulation of multiple antioxidative defense mechanisms, both spatially and temporarily, to protect H. pluvialis cells against oxidative stress. © Springer-Verlag 2004.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_HK
dc.relation.ispartofPlantaen_HK
dc.subjectAstaxanthinen_HK
dc.subjectHaematococcusen_HK
dc.subjectOxidative stressen_HK
dc.subjectProtein expressionen_HK
dc.subjectProteomicsen_HK
dc.subjectStress responseen_HK
dc.titleProteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0032-0935&volume=220&spage=17&epage=29&date=2004&atitle=Proteomic+analysis+of+molecular+response+to+oxidative+stress+by+the+green+alga+Haematococcus+pluvialis+(Chlorophyceae)en_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00425-004-1323-5en_HK
dc.identifier.pmid15258760-
dc.identifier.scopuseid_2-s2.0-11144320702en_HK
dc.identifier.hkuros104982en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-11144320702&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume220en_HK
dc.identifier.issue1en_HK
dc.identifier.spage17en_HK
dc.identifier.epage29en_HK
dc.identifier.isiWOS:000224938000003-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridWang, SB=7410347419en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridSommerfeld, M=7007025132en_HK
dc.identifier.scopusauthoridHu, Q=26666082400en_HK
dc.identifier.citeulike652737-

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