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Article: Continuous cultivation of the diatom Nitzschia laevis for eicosapentaenoic acid production: Physiological study and process optimization

TitleContinuous cultivation of the diatom Nitzschia laevis for eicosapentaenoic acid production: Physiological study and process optimization
Authors
Issue Date2002
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/bp
Citation
Biotechnology Progress, 2002, v. 18 n. 1, p. 21-28 How to Cite?
AbstractThe continuous cultures of the diatom Nitzschia laevis were performed at different dilution rates (D) and feed glucose concentrations (S o) to investigate cellular physiological responses and its production potential of eicosapentaenoic acid (EPA). Steadystate cell dry weight, residual glucose concentration, cell growth yield, specific glucose consumption rate, and fatty acid profiles were investigated within the range of D from 0.1 to 1.0 day -1 (S 0 fixed at 20 g/L) and the range of S 0 from 5 to 35 g/L (D fixed at 0.3 day -1), respectively. The highest EPA productivity of 73 mg L -1 day -1 was obtained at D = 0.5 day -1 and S 0 = 20 g/L. However, when the continuous culture achieved high productivities of EPA at certain dilution rates and feed glucose concentrations, glucose m the feed could not be consumed completely. Accordingly, the continuous culture was evaluated in terms of both EPA productivity (P) and glucose assimilation efficiency (E). The parameter η, defined as the product of P and E, was used as an overall performance index. Since η is a function of the two independent variables D and S 0, we employed a central composite design to optimize D and S 0 for the highest η value. Based on the experimental results of the design, a second-order polynomial equation was established to represent the relationship between η and D and S 0. The optimal values old and S 0 were subsequently determined as 0.481 day -1 and 15.56 g/L, respectively by the empirical model. The verification experiment confirmed the validity of the model. Under the optimal conditions, η value reached 46.5 mg L -1 day -1, suggesting a considerably high efficiency of the continuous culture of N. laevis in terms of EPA production and glucose utilization.
Persistent Identifierhttp://hdl.handle.net/10722/68599
ISSN
2015 Impact Factor: 2.167
2015 SCImago Journal Rankings: 0.736
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWen, ZYen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:06:01Z-
dc.date.available2010-09-06T06:06:01Z-
dc.date.issued2002en_HK
dc.identifier.citationBiotechnology Progress, 2002, v. 18 n. 1, p. 21-28en_HK
dc.identifier.issn8756-7938en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68599-
dc.description.abstractThe continuous cultures of the diatom Nitzschia laevis were performed at different dilution rates (D) and feed glucose concentrations (S o) to investigate cellular physiological responses and its production potential of eicosapentaenoic acid (EPA). Steadystate cell dry weight, residual glucose concentration, cell growth yield, specific glucose consumption rate, and fatty acid profiles were investigated within the range of D from 0.1 to 1.0 day -1 (S 0 fixed at 20 g/L) and the range of S 0 from 5 to 35 g/L (D fixed at 0.3 day -1), respectively. The highest EPA productivity of 73 mg L -1 day -1 was obtained at D = 0.5 day -1 and S 0 = 20 g/L. However, when the continuous culture achieved high productivities of EPA at certain dilution rates and feed glucose concentrations, glucose m the feed could not be consumed completely. Accordingly, the continuous culture was evaluated in terms of both EPA productivity (P) and glucose assimilation efficiency (E). The parameter η, defined as the product of P and E, was used as an overall performance index. Since η is a function of the two independent variables D and S 0, we employed a central composite design to optimize D and S 0 for the highest η value. Based on the experimental results of the design, a second-order polynomial equation was established to represent the relationship between η and D and S 0. The optimal values old and S 0 were subsequently determined as 0.481 day -1 and 15.56 g/L, respectively by the empirical model. The verification experiment confirmed the validity of the model. Under the optimal conditions, η value reached 46.5 mg L -1 day -1, suggesting a considerably high efficiency of the continuous culture of N. laevis in terms of EPA production and glucose utilization.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/bpen_HK
dc.relation.ispartofBiotechnology Progressen_HK
dc.titleContinuous cultivation of the diatom Nitzschia laevis for eicosapentaenoic acid production: Physiological study and process optimizationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=8756-7938&volume=18&spage=21&epage=28&date=2002&atitle=Continuous+cultivation+of+the+diatom+Nitzschia+laevis+for+eicosapentaenoic+acid+production:+physiological+study+and+process+optimizationen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/bp010125nen_HK
dc.identifier.scopuseid_2-s2.0-0036007985en_HK
dc.identifier.hkuros72373en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036007985&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue1en_HK
dc.identifier.spage21en_HK
dc.identifier.epage28en_HK
dc.identifier.isiWOS:000173819100003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWen, ZY=14012373200en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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