File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: High cell density mixotrophic culture of Spirulina platensis on glucose for phycocyanin production using a fed-batch system

TitleHigh cell density mixotrophic culture of Spirulina platensis on glucose for phycocyanin production using a fed-batch system
Authors
KeywordsFed-batch system
Glucose
High cell density
Microalgae
Mixotrophic culture
Phycocyanin production
Spirulina platensis
Issue Date1997
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictec
Citation
Enzyme And Microbial Technology, 1997, v. 20 n. 3, p. 221-224 How to Cite?
AbstractA mixotrophic culture might be used as an alternative to conventional photoautotrophic mass culture systems for production of high value chemicals and pharmaceutical by Spirulina platensis. The possibility of using mixotrophic culture to achieve high cell densities and phycocyanin productivities was investigated using fed-batch culture in a 3.7-l fermentor. In fed-batch cultures, the highest cell concentration of 10.24 g l-1 (dry weight) and the highest phycocyanin production of 795 mg l-1 were achieved which were much higher than those reported in the literature. The highest biomass concentration and phycocyanin production in the mixotrophic fed-batch culture were 5.1-fold and 2.87-fold, respectively, of that obtained in the photoautotrophic batch culture using the same fermentor. The cellular phycocyanin content was enhanced with increasing photosynthetic activities. In the photoautotrophic batch culture, the phycocyanin content was constant at approximately 135 mg g-1 dry cells throughout the course of cultivation. In contrast, in the mixotrophic batch culture, the phycocyanin content was not constant. It increased from 54 mg g-1 to 125 g-1 dry cells during the course of cultivation presumably due to the continuous changes of heterotrophic to photoautotrophic domination. These results suggest that the maximum phycocyanin production should occur at the best combination of cell densities and photosynthetic activities in mixotrophic fed-batch cultures of the microalga. | A mixotrophic culture might be used as an alternative to conventional photoautotrophic mass culture systems for production of high value chemicals and pharmaceuticals by Spirulina platensis. The possibility of using mixotrophic culture to achieve high cell densities and phycocyanin productivities was investigated using fed-batch culture in a 3.7-l fermentor. In fed-batch cultures, the highest cell concentration of 10.24 g l-1 (dry weight) and the highest phycocyanin production of 795 mg l-1 were achieved which were much higher than those reported in the literature. The highest biomass concentration and phycocyanin production in the mixotrophic fed-batch culture were 5.1-fold and 2.8-fold, respectively, of that obtained in the photoautotrophic batch culture using the same fermentor. The cellular phycocyanin content was enhanced with increasing photosynthetic activities. In the photoautotrophic batch culture, the phycocyanin content was constant at approximately 135 mg g-1 dry cells throughout the course of cultivation. In contrast, in the mixotrophic batch culture, the phycocyanin content was not constant. It increased from 54 mg g-1 to 125 g-1 dry cells during the course of cultivation presumably due to the continuous changes of heterotrophic to photoautotrophic domination. These results suggest that the maximum phycocyanin production should occur at the best combination of cell densities and photosynthetic activities in mixotrophic fed-batch cultures of the microalga.
Persistent Identifierhttp://hdl.handle.net/10722/68597
ISSN
2015 Impact Factor: 2.624
2015 SCImago Journal Rankings: 0.854
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Fen_HK
dc.contributor.authorZhang, Yen_HK
dc.date.accessioned2010-09-06T06:06:00Z-
dc.date.available2010-09-06T06:06:00Z-
dc.date.issued1997en_HK
dc.identifier.citationEnzyme And Microbial Technology, 1997, v. 20 n. 3, p. 221-224en_HK
dc.identifier.issn0141-0229en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68597-
dc.description.abstractA mixotrophic culture might be used as an alternative to conventional photoautotrophic mass culture systems for production of high value chemicals and pharmaceutical by Spirulina platensis. The possibility of using mixotrophic culture to achieve high cell densities and phycocyanin productivities was investigated using fed-batch culture in a 3.7-l fermentor. In fed-batch cultures, the highest cell concentration of 10.24 g l-1 (dry weight) and the highest phycocyanin production of 795 mg l-1 were achieved which were much higher than those reported in the literature. The highest biomass concentration and phycocyanin production in the mixotrophic fed-batch culture were 5.1-fold and 2.87-fold, respectively, of that obtained in the photoautotrophic batch culture using the same fermentor. The cellular phycocyanin content was enhanced with increasing photosynthetic activities. In the photoautotrophic batch culture, the phycocyanin content was constant at approximately 135 mg g-1 dry cells throughout the course of cultivation. In contrast, in the mixotrophic batch culture, the phycocyanin content was not constant. It increased from 54 mg g-1 to 125 g-1 dry cells during the course of cultivation presumably due to the continuous changes of heterotrophic to photoautotrophic domination. These results suggest that the maximum phycocyanin production should occur at the best combination of cell densities and photosynthetic activities in mixotrophic fed-batch cultures of the microalga. | A mixotrophic culture might be used as an alternative to conventional photoautotrophic mass culture systems for production of high value chemicals and pharmaceuticals by Spirulina platensis. The possibility of using mixotrophic culture to achieve high cell densities and phycocyanin productivities was investigated using fed-batch culture in a 3.7-l fermentor. In fed-batch cultures, the highest cell concentration of 10.24 g l-1 (dry weight) and the highest phycocyanin production of 795 mg l-1 were achieved which were much higher than those reported in the literature. The highest biomass concentration and phycocyanin production in the mixotrophic fed-batch culture were 5.1-fold and 2.8-fold, respectively, of that obtained in the photoautotrophic batch culture using the same fermentor. The cellular phycocyanin content was enhanced with increasing photosynthetic activities. In the photoautotrophic batch culture, the phycocyanin content was constant at approximately 135 mg g-1 dry cells throughout the course of cultivation. In contrast, in the mixotrophic batch culture, the phycocyanin content was not constant. It increased from 54 mg g-1 to 125 g-1 dry cells during the course of cultivation presumably due to the continuous changes of heterotrophic to photoautotrophic domination. These results suggest that the maximum phycocyanin production should occur at the best combination of cell densities and photosynthetic activities in mixotrophic fed-batch cultures of the microalga.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictecen_HK
dc.relation.ispartofEnzyme and Microbial Technologyen_HK
dc.rightsEnzyme and Microbial Technology. Copyright © Elsevier Inc.en_HK
dc.subjectFed-batch systemen_HK
dc.subjectGlucoseen_HK
dc.subjectHigh cell densityen_HK
dc.subjectMicroalgaeen_HK
dc.subjectMixotrophic cultureen_HK
dc.subjectPhycocyanin productionen_HK
dc.subjectSpirulina platensisen_HK
dc.titleHigh cell density mixotrophic culture of Spirulina platensis on glucose for phycocyanin production using a fed-batch systemen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-0229&volume=20&spage=221&epage=224&date=1997&atitle=High+cell+density+mixotrophic+culture+of+Spirulina+platensis+on+glucose+for+phycocyanin+production+using+a+fed-batch+systemen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0141-0229(96)00116-0en_HK
dc.identifier.scopuseid_2-s2.0-0031568791en_HK
dc.identifier.hkuros23375en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031568791&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue3en_HK
dc.identifier.spage221en_HK
dc.identifier.epage224en_HK
dc.identifier.isiWOS:A1997WM69900009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridZhang, Y=23089593700en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats