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Article: Separation and purification of phycocyanins from Spirulina platensis in mixotrophic culture

TitleSeparation and purification of phycocyanins from Spirulina platensis in mixotrophic culture
混合异养螺旋藻中藻蓝蛋白的分离和纯化
Authors
KeywordsSeparation
Spirulina platensis
Purification
Phycocyanin
Issue Date1999
PublisherSouth China University of Technology. The Journal's web site is located at http://www.cnki.com.cn/Journal/C-C8-HNLG.htm
Citation
華南理工大學學報(自然科學版), 1999, v. 27 n. 4, p. 32-36 How to Cite?
Journal of South China University of Technology (Natural Science Edition), 1999, v. 27 n. 4, p. 32-36 How to Cite?
AbstractFor phycocyanin separation and purification of Spirulina platensis, a method using fraction precipitation of ammonium sulphate, ion exchange and gel filtration chromatography was investigated. In fraction precipitation of ammonium sulphate, it was found that by adsorption spectra scan of precipitates, 300 g/ L ammonium sulphate solution could remove other proteins well while 500 g/ L and 650 g/ L ammonium sulphate solutions could separate c_phycocy anin and allophycocy anin well. After ion exchange chromatography and gel filtration chromatography, finally , the absorbance ratios, reflecting the purities of c_phy cocyanin and allophy cocyanin, reached 5.06 and 5 .34 respectively .
Persistent Identifierhttp://hdl.handle.net/10722/68576
ISSN
2015 SCImago Journal Rankings: 0.200

 

DC FieldValueLanguage
dc.contributor.authorZhang, YMen_HK
dc.contributor.authorGuo, SYen_HK
dc.contributor.authorChen, SFen_HK
dc.date.accessioned2010-09-06T06:05:49Z-
dc.date.available2010-09-06T06:05:49Z-
dc.date.issued1999en_HK
dc.identifier.citation華南理工大學學報(自然科學版), 1999, v. 27 n. 4, p. 32-36en_HK
dc.identifier.citationJournal of South China University of Technology (Natural Science Edition), 1999, v. 27 n. 4, p. 32-36-
dc.identifier.issn1000-565Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68576-
dc.description.abstractFor phycocyanin separation and purification of Spirulina platensis, a method using fraction precipitation of ammonium sulphate, ion exchange and gel filtration chromatography was investigated. In fraction precipitation of ammonium sulphate, it was found that by adsorption spectra scan of precipitates, 300 g/ L ammonium sulphate solution could remove other proteins well while 500 g/ L and 650 g/ L ammonium sulphate solutions could separate c_phycocy anin and allophycocy anin well. After ion exchange chromatography and gel filtration chromatography, finally , the absorbance ratios, reflecting the purities of c_phy cocyanin and allophy cocyanin, reached 5.06 and 5 .34 respectively .-
dc.languageengen_HK
dc.publisherSouth China University of Technology. The Journal's web site is located at http://www.cnki.com.cn/Journal/C-C8-HNLG.htmen_HK
dc.relation.ispartof華南理工大學學報(自然科學版)en_HK
dc.relation.ispartofJournal of South China University of Technology (Natural Science Edition)-
dc.subjectSeparation-
dc.subjectSpirulina platensis-
dc.subjectPurification-
dc.subjectPhycocyanin-
dc.titleSeparation and purification of phycocyanins from Spirulina platensis in mixotrophic cultureen_HK
dc.title混合异养螺旋藻中藻蓝蛋白的分离和纯化-
dc.typeArticleen_HK
dc.identifier.emailChen, SF: sfchen@hkusua.hku.hken_HK
dc.identifier.authorityChen, SF=rp00672en_HK
dc.identifier.hkuros45474en_HK
dc.identifier.volume27-
dc.identifier.issue4-
dc.identifier.spage32-
dc.identifier.epage36-
dc.publisher.placeChina-

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