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Article: Isolation and purification of canthaxanthin from the microalga Chlorella zofingiensis by high-speed counter-current chromatography

TitleIsolation and purification of canthaxanthin from the microalga Chlorella zofingiensis by high-speed counter-current chromatography
Authors
KeywordsCanthaxanthin
Chlorella zofingiensis
Counter-current chromatography
Issue Date2006
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jss
Citation
Journal Of Separation Science, 2006, v. 29 n. 5, p. 699-703 How to Cite?
AbstractCertain microalgae are considered to be a potential source of canthaxanthin, which possesses strong antioxidant and anticancer activities. A high-speed counter-current chromatography (HSCCC) method was developed for the separation and purification of canthaxanthin from the microalga Chlorella zofingiensis. The crude canthaxanthin was obtained by extraction with organic solvents after the microalgal sample had been saponified. Preparative HSCCC, with a two-phase solvent system composed of n-hexane-ethanol-water (10:9:1 v/v), was successfully performed yielding canthaxanthin at 98.7% purity from 150 mg of the crude extract (2.1% canthaxanthin) in a one-step separation. The recovery of canthaxanthin was 92.3%. This was the first report that canthaxanthin was successfully separated and purified from microalgae. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/68572
ISSN
2015 Impact Factor: 2.741
2015 SCImago Journal Rankings: 1.041
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorFan, KWen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:05:47Z-
dc.date.available2010-09-06T06:05:47Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Separation Science, 2006, v. 29 n. 5, p. 699-703en_HK
dc.identifier.issn1615-9306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68572-
dc.description.abstractCertain microalgae are considered to be a potential source of canthaxanthin, which possesses strong antioxidant and anticancer activities. A high-speed counter-current chromatography (HSCCC) method was developed for the separation and purification of canthaxanthin from the microalga Chlorella zofingiensis. The crude canthaxanthin was obtained by extraction with organic solvents after the microalgal sample had been saponified. Preparative HSCCC, with a two-phase solvent system composed of n-hexane-ethanol-water (10:9:1 v/v), was successfully performed yielding canthaxanthin at 98.7% purity from 150 mg of the crude extract (2.1% canthaxanthin) in a one-step separation. The recovery of canthaxanthin was 92.3%. This was the first report that canthaxanthin was successfully separated and purified from microalgae. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jssen_HK
dc.relation.ispartofJournal of Separation Scienceen_HK
dc.subjectCanthaxanthinen_HK
dc.subjectChlorella zofingiensisen_HK
dc.subjectCounter-current chromatographyen_HK
dc.titleIsolation and purification of canthaxanthin from the microalga Chlorella zofingiensis by high-speed counter-current chromatographyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9306&volume=29&issue=5&spage=699&epage=703&date=2006&atitle=Isolation+and+purification+of+canthaxanthin+from+the+microalga+Chlorella+zofingiensis+by+high-speed+counter-current+chromatographyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jssc.200500365en_HK
dc.identifier.pmid16605091-
dc.identifier.scopuseid_2-s2.0-33645736301en_HK
dc.identifier.hkuros122407en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645736301&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue5en_HK
dc.identifier.spage699en_HK
dc.identifier.epage703en_HK
dc.identifier.isiWOS:000236486500016-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridFan, KW=7202978325en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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