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Article: Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection

TitleDetermination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection
Authors
KeywordsFluorescence detection
High performance liquid chromatography
Microalgae
Paralytic shellfish poisoning toxins
Issue Date2005
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
Citation
Analytical And Bioanalytical Chemistry, 2005, v. 383 n. 6, p. 1014-1017 How to Cite?
AbstractA novel method for the determination of paralytic shellfish poisoning (PSP) toxins using high-performance liquid chromatography with fluorescence detection was developed. The fluorescent derivates of neosaxitoxin (neoSTX), saxitoxin (STX), gonyautoxins 1 and 4 (GTX1+4), and gonyautoxins 2 and 3 (GTX2+3) were separated on a μBondapak NH 2 column (300 mm × 3.9 mm, 10 μm) using water and acetate buffer (pH 6.5) as the mobile phase (1.00 mL min -1) in gradient mode with fluorescence detection at 390 nm (excitation at 330 nm). The linear ranges of neoSTX, STX, GTX1+4 and GTX2+3 were 3.31-331, 0.952-95.2, 3.78-378 and 0.124-12.4 ng mL -1, respectively. The detection limits of neoSTX, STX, GTX1+4 and GTX2+3 were 1.10, 0.32, 1.26 and 0.041 ng mL -1, respectively. The method was successfully applied to the determination of PSP toxins in microalgae. The recoveries ranged from 88±2% to 107±4% and the relative standard deviations were 0.16% to 4.4%. The procedure is also environmentally friendly because no organic solvent is used in the mobile phase. © Springer-Verlag 2005.
Persistent Identifierhttp://hdl.handle.net/10722/68554
ISSN
2015 Impact Factor: 3.125
2015 SCImago Journal Rankings: 1.096
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHe, HZen_HK
dc.contributor.authorLi, HBen_HK
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:05:38Z-
dc.date.available2010-09-06T06:05:38Z-
dc.date.issued2005en_HK
dc.identifier.citationAnalytical And Bioanalytical Chemistry, 2005, v. 383 n. 6, p. 1014-1017en_HK
dc.identifier.issn1618-2642en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68554-
dc.description.abstractA novel method for the determination of paralytic shellfish poisoning (PSP) toxins using high-performance liquid chromatography with fluorescence detection was developed. The fluorescent derivates of neosaxitoxin (neoSTX), saxitoxin (STX), gonyautoxins 1 and 4 (GTX1+4), and gonyautoxins 2 and 3 (GTX2+3) were separated on a μBondapak NH 2 column (300 mm × 3.9 mm, 10 μm) using water and acetate buffer (pH 6.5) as the mobile phase (1.00 mL min -1) in gradient mode with fluorescence detection at 390 nm (excitation at 330 nm). The linear ranges of neoSTX, STX, GTX1+4 and GTX2+3 were 3.31-331, 0.952-95.2, 3.78-378 and 0.124-12.4 ng mL -1, respectively. The detection limits of neoSTX, STX, GTX1+4 and GTX2+3 were 1.10, 0.32, 1.26 and 0.041 ng mL -1, respectively. The method was successfully applied to the determination of PSP toxins in microalgae. The recoveries ranged from 88±2% to 107±4% and the relative standard deviations were 0.16% to 4.4%. The procedure is also environmentally friendly because no organic solvent is used in the mobile phase. © Springer-Verlag 2005.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htmen_HK
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_HK
dc.subjectFluorescence detectionen_HK
dc.subjectHigh performance liquid chromatographyen_HK
dc.subjectMicroalgaeen_HK
dc.subjectParalytic shellfish poisoning toxinsen_HK
dc.titleDetermination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1618-2642&volume=383&spage=1014&epage=1017&date=2005&atitle=Determination+of+paralytic+shellfish+poisoning+toxins+in+cultured+microalgae+by+high-performance+liquid+chromatography+with+fluorescence+detectionen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00216-005-0092-8en_HK
dc.identifier.pmid16231133-
dc.identifier.scopuseid_2-s2.0-27744492505en_HK
dc.identifier.hkuros122440en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27744492505&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume383en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1014en_HK
dc.identifier.epage1017en_HK
dc.identifier.isiWOS:000233875200017-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridHe, HZ=55214936100en_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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