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Article: Preparative isolation and purification of bergapten and imperatorin from the medicinal plant Cnidium monnieri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase

TitlePreparative isolation and purification of bergapten and imperatorin from the medicinal plant Cnidium monnieri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase
Authors
KeywordsBergapten
Cnidium monnieri
Counter-current chromatography
Imperatorin
Pharmaceutical analysis
Plant materials
Preparative chromatography
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2004, v. 1061 n. 1, p. 51-54 How to Cite?
AbstractA high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of bergapten and imperatorin from the Chinese medicinal plant Cnidium monnieri (L.) Cusson. The crude extract was obtained by extraction with ethanol from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:5:5, v/v/v/v) was successfully performed by increasing the flow-rate of the mobile phase stepwise from 1.0 to 2.0 ml min -1 after 180 min. The components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 45.8 mg of bergapten at 96.5% purity and 118.3 mg of imperatorin at 98.2% purity from 500 mg of the crude extract in a single run. The recoveries of bergapten and imperatorin were 92.1 and 93.7%, respectively. © 2004 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68546
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:05:34Z-
dc.date.available2010-09-06T06:05:34Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Chromatography A, 2004, v. 1061 n. 1, p. 51-54en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68546-
dc.description.abstractA high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of bergapten and imperatorin from the Chinese medicinal plant Cnidium monnieri (L.) Cusson. The crude extract was obtained by extraction with ethanol from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:5:5, v/v/v/v) was successfully performed by increasing the flow-rate of the mobile phase stepwise from 1.0 to 2.0 ml min -1 after 180 min. The components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 45.8 mg of bergapten at 96.5% purity and 118.3 mg of imperatorin at 98.2% purity from 500 mg of the crude extract in a single run. The recoveries of bergapten and imperatorin were 92.1 and 93.7%, respectively. © 2004 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectBergaptenen_HK
dc.subjectCnidium monnierien_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectImperatorinen_HK
dc.subjectPharmaceutical analysisen_HK
dc.subjectPlant materialsen_HK
dc.subjectPreparative chromatographyen_HK
dc.titlePreparative isolation and purification of bergapten and imperatorin from the medicinal plant Cnidium monnieri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1061&spage=51&epage=54&date=2004&atitle=Preparative+isolation+and+purification+of+bergapten+and+imperatorin+from+the+medicinal+plant+Cnidium+monnieri+using+high-speed+counter-current+chromatography+by+stepwise+increasing+the+flow-rate+of+the+mobile+phaseen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2004.10.091en_HK
dc.identifier.pmid15633744-
dc.identifier.scopuseid_2-s2.0-9644252773en_HK
dc.identifier.hkuros104977en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-9644252773&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1061en_HK
dc.identifier.issue1en_HK
dc.identifier.spage51en_HK
dc.identifier.epage54en_HK
dc.identifier.isiWOS:000225746500007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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