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Article: Simultaneous determination of carotenoids and chlorophylls in algae by high performance liquid chromatography

TitleSimultaneous determination of carotenoids and chlorophylls in algae by high performance liquid chromatography
高效液相色譜法測定藻類中的類胡蘿卜素和葉綠素
Authors
KeywordsHigh performance liquid chromatography
Carotenoids
Chlorophylls
Algae
Issue Date1997
Publisher科学出版社 (The Science Press). The Journal's web site is located at http://www.chrom-china.com/
Citation
色譜 , 1997, v. 15 n. 2, p. 133-135 How to Cite?
Chinese Journal of Chromatography, 1997, v. 15 n. 2, p. 133-135 How to Cite?
AbstractAn isocratic reversed-phase HPLC method for the simultaneous determination of carotenoids and chlorophylls in algae is presented. Both carotenoids and chlorophylls were separated by using a C18 column and a mobile phase consisting of d ichloromethane/acetonitrile/methanol/water (22.5 : 9.5: 67. 5:0.5 ). The flow rate was 1. 0mL/min. These pigments were detected by a UV-VIS absorbance detector set at 450nm. The average recoveries of these pigments by standard addition method were 99. 1% for lutein,98. 5 % for a- carotene, 99. 4% for β- carotene, 100. 6% for chlorophyll a and 99. 9% for chlorophyll b. The relative standard deviations of these pigments were 2. 4% for lutein, 5. 6% for α- carotene, 6.0% for β-carotene, 4. 1 % for chlorophyll a and 4. 0% for chlorophyll b. The calibration curves for these pigments gave good linearity over a wide range of o-50mg/L (r=0.9992-0. 9999), The retention times were 3.0 min for lutein, 4. 7 min for chlorophyll b, 6. 3 min for chlorophyll a, 14. 1 min for α- carotene, and 14. 7 min for β-carotene. Using this method, carotenoids and chlorophylls were determined in Spirulina Platensis, Chlorella vulgaris and Haematococcus lacustris. Chlorella vulgaris and Haematococcus lacustris samples were ground in a B. Braun homogenizer. Spirulina platensis sample was smashed by supersonic wave, Acetone was used as extractant.
Persistent Identifierhttp://hdl.handle.net/10722/68540
ISSN
2015 SCImago Journal Rankings: 0.225

 

DC FieldValueLanguage
dc.contributor.authorYuan, JPen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorShi, XMen_HK
dc.contributor.authorGong, XDen_HK
dc.contributor.authorChen, SFen_HK
dc.date.accessioned2010-09-06T06:05:31Z-
dc.date.available2010-09-06T06:05:31Z-
dc.date.issued1997en_HK
dc.identifier.citation色譜 , 1997, v. 15 n. 2, p. 133-135en_HK
dc.identifier.citationChinese Journal of Chromatography, 1997, v. 15 n. 2, p. 133-135-
dc.identifier.issn1000-8713-
dc.identifier.urihttp://hdl.handle.net/10722/68540-
dc.description.abstractAn isocratic reversed-phase HPLC method for the simultaneous determination of carotenoids and chlorophylls in algae is presented. Both carotenoids and chlorophylls were separated by using a C18 column and a mobile phase consisting of d ichloromethane/acetonitrile/methanol/water (22.5 : 9.5: 67. 5:0.5 ). The flow rate was 1. 0mL/min. These pigments were detected by a UV-VIS absorbance detector set at 450nm. The average recoveries of these pigments by standard addition method were 99. 1% for lutein,98. 5 % for a- carotene, 99. 4% for β- carotene, 100. 6% for chlorophyll a and 99. 9% for chlorophyll b. The relative standard deviations of these pigments were 2. 4% for lutein, 5. 6% for α- carotene, 6.0% for β-carotene, 4. 1 % for chlorophyll a and 4. 0% for chlorophyll b. The calibration curves for these pigments gave good linearity over a wide range of o-50mg/L (r=0.9992-0. 9999), The retention times were 3.0 min for lutein, 4. 7 min for chlorophyll b, 6. 3 min for chlorophyll a, 14. 1 min for α- carotene, and 14. 7 min for β-carotene. Using this method, carotenoids and chlorophylls were determined in Spirulina Platensis, Chlorella vulgaris and Haematococcus lacustris. Chlorella vulgaris and Haematococcus lacustris samples were ground in a B. Braun homogenizer. Spirulina platensis sample was smashed by supersonic wave, Acetone was used as extractant.-
dc.languagechien_HK
dc.publisher科学出版社 (The Science Press). The Journal's web site is located at http://www.chrom-china.com/-
dc.relation.ispartof色譜en_HK
dc.relation.ispartofChinese Journal of Chromatography-
dc.subjectHigh performance liquid chromatography-
dc.subjectCarotenoids-
dc.subjectChlorophylls-
dc.subjectAlgae-
dc.titleSimultaneous determination of carotenoids and chlorophylls in algae by high performance liquid chromatographyen_HK
dc.title高效液相色譜法測定藻類中的類胡蘿卜素和葉綠素-
dc.typeArticleen_HK
dc.identifier.emailChen, SF: sfchen@hkusua.hku.hken_HK
dc.identifier.authorityChen, SF=rp00672en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros21414en_HK
dc.identifier.volume15-
dc.identifier.issue2-
dc.identifier.spage133-
dc.identifier.epage135-
dc.publisher.placeChina-

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