File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization

TitleSecretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization
Authors
KeywordsBacillus
Phosphorus
Phytase
Phytic acid
Root
Secretion
Issue Date2005
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/plantsci
Citation
Plant Science, 2005, v. 169 n. 2, p. 341-349 How to Cite?
AbstractA large proportion of soil phosphorus (P) exists as organic compounds, of which phytic acid (IHP) is the dominant form. To generate transgenic plants capable of utilizing exogenous IHP, β-propeller phytase from Bacillus subtilis (168phyA) was constitutively expressed in tobacco and Arabidopsis, and was shown to be secreted from their roots. In tobacco, phytase activities in transgenic leaf and root extracts were seven to nine times higher than those in wild-type extracts; whereas, the extracellular phytase activities of transgenic plants were enhanced by four to six times. In sterile hydroponic culture using 1 mM Na-IHP as the sole P source, the transgenic tobacco lines accumulated 1.7-2.2 times more shoot biomass than the wild-type plants after 30 days of growth, concomitant with a 27-36% increase in shoot P concentration. Similar results were observed from the transgenic Arabidopsis. Our work on the exudation of recombinant Bacillus phytase from plant roots may offer a new perspective on mobilizing soil phytate into inorganic phosphate for plant uptake. © 2005 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68529
ISSN
2015 Impact Factor: 3.362
2015 SCImago Journal Rankings: 1.601
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLung, SCen_HK
dc.contributor.authorChan, WLen_HK
dc.contributor.authorYip, Wen_HK
dc.contributor.authorWang, Len_HK
dc.contributor.authorYeung, ECen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2010-09-06T06:05:25Z-
dc.date.available2010-09-06T06:05:25Z-
dc.date.issued2005en_HK
dc.identifier.citationPlant Science, 2005, v. 169 n. 2, p. 341-349en_HK
dc.identifier.issn0168-9452en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68529-
dc.description.abstractA large proportion of soil phosphorus (P) exists as organic compounds, of which phytic acid (IHP) is the dominant form. To generate transgenic plants capable of utilizing exogenous IHP, β-propeller phytase from Bacillus subtilis (168phyA) was constitutively expressed in tobacco and Arabidopsis, and was shown to be secreted from their roots. In tobacco, phytase activities in transgenic leaf and root extracts were seven to nine times higher than those in wild-type extracts; whereas, the extracellular phytase activities of transgenic plants were enhanced by four to six times. In sterile hydroponic culture using 1 mM Na-IHP as the sole P source, the transgenic tobacco lines accumulated 1.7-2.2 times more shoot biomass than the wild-type plants after 30 days of growth, concomitant with a 27-36% increase in shoot P concentration. Similar results were observed from the transgenic Arabidopsis. Our work on the exudation of recombinant Bacillus phytase from plant roots may offer a new perspective on mobilizing soil phytate into inorganic phosphate for plant uptake. © 2005 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/plantscien_HK
dc.relation.ispartofPlant Scienceen_HK
dc.rightsPlant Science. Copyright © Elsevier Ireland Ltd.en_HK
dc.subjectBacillusen_HK
dc.subjectPhosphorusen_HK
dc.subjectPhytaseen_HK
dc.subjectPhytic aciden_HK
dc.subjectRooten_HK
dc.subjectSecretionen_HK
dc.titleSecretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilizationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-9452&volume=169&spage=341&epage=349&date=2005&atitle=Secretion+of+beta-propeller+phytase+from+tobacco+and+Arabidopsis+roots+enhances+phosphorus+utilizationen_HK
dc.identifier.emailYip, W: wkyip@hkucc.hku.hken_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityYip, W=rp00833en_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.plantsci.2005.03.006en_HK
dc.identifier.scopuseid_2-s2.0-21444439925en_HK
dc.identifier.hkuros98809en_HK
dc.identifier.hkuros163713-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-21444439925&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume169en_HK
dc.identifier.issue2en_HK
dc.identifier.spage341en_HK
dc.identifier.epage349en_HK
dc.identifier.isiWOS:000230938500007-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridLung, SC=8619522500en_HK
dc.identifier.scopusauthoridChan, WL=8619522000en_HK
dc.identifier.scopusauthoridYip, W=7102784428en_HK
dc.identifier.scopusauthoridWang, L=7409175686en_HK
dc.identifier.scopusauthoridYeung, EC=7101971768en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.citeulike2282839-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats