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Article: Actin visualization in living immature pollen of rice using a GFP-mouse talin fusion protein

TitleActin visualization in living immature pollen of rice using a GFP-mouse talin fusion protein
Authors
KeywordsActin
Green fluorescent protein (GFP)
Microfilament
Mouse talin
Oryza sativa
Pollen
Issue Date2002
Citation
Acta Botanica Sinica, 2002, v. 44 n. 6, p. 642-648 How to Cite?
Abstract
Green flourescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants. Actin microfilaments could be visualized only in the late-developmental stage of the immature pollen. During this developmental stage, microfilaments, initially composed of very short fibrils, develop into a very complex and novel network that sometimes totally and sometimes partially encloses the vegetative nucleus and the spherical shaped generative cell in the central cytoplasm of the immature pollen. The behavior of the actin microfilamentous structure throughout the late-developmental stage of the immature pollen is extremely dynamic, and the likelihood of this structure in generating forces for vegetative nucleus and generative cell movement in the immature pollen has been discussed. No actin filaments were visualized in the spherical generative cells.
Persistent Identifierhttp://hdl.handle.net/10722/68518
ISSN
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorXu, SXen_HK
dc.contributor.authorWang, LJen_HK
dc.contributor.authorQiu, ZPen_HK
dc.contributor.authorYe, YJen_HK
dc.contributor.authorYu, XHen_HK
dc.date.accessioned2010-09-06T06:05:19Z-
dc.date.available2010-09-06T06:05:19Z-
dc.date.issued2002en_HK
dc.identifier.citationActa Botanica Sinica, 2002, v. 44 n. 6, p. 642-648en_HK
dc.identifier.issn0577-7496en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68518-
dc.description.abstractGreen flourescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants. Actin microfilaments could be visualized only in the late-developmental stage of the immature pollen. During this developmental stage, microfilaments, initially composed of very short fibrils, develop into a very complex and novel network that sometimes totally and sometimes partially encloses the vegetative nucleus and the spherical shaped generative cell in the central cytoplasm of the immature pollen. The behavior of the actin microfilamentous structure throughout the late-developmental stage of the immature pollen is extremely dynamic, and the likelihood of this structure in generating forces for vegetative nucleus and generative cell movement in the immature pollen has been discussed. No actin filaments were visualized in the spherical generative cells.en_HK
dc.languageengen_HK
dc.relation.ispartofActa Botanica Sinicaen_HK
dc.subjectActinen_HK
dc.subjectGreen fluorescent protein (GFP)en_HK
dc.subjectMicrofilamenten_HK
dc.subjectMouse talinen_HK
dc.subjectOryza sativaen_HK
dc.subjectPollenen_HK
dc.titleActin visualization in living immature pollen of rice using a GFP-mouse talin fusion proteinen_HK
dc.typeArticleen_HK
dc.identifier.emailYe, YJ: wkyip@hkucc.hku.hken_HK
dc.identifier.authorityYe, YJ=rp00833en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0036329819en_HK
dc.identifier.hkuros67987en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036329819&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume44en_HK
dc.identifier.issue6en_HK
dc.identifier.spage642en_HK
dc.identifier.epage648en_HK
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridXu, SX=7404438821en_HK
dc.identifier.scopusauthoridWang, LJ=7409175686en_HK
dc.identifier.scopusauthoridQiu, ZP=7202941720en_HK
dc.identifier.scopusauthoridYe, YJ=7102784428en_HK
dc.identifier.scopusauthoridYu, XH=37861474600en_HK

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