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Article: Actin visualization in living immature pollen of rice using a GFP-mouse talin fusion protein
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TitleActin visualization in living immature pollen of rice using a GFP-mouse talin fusion protein
 
AuthorsXu, SX1
Wang, LJ1
Qiu, ZP1
Ye, YJ1
Yu, XH1
 
KeywordsActin
Green fluorescent protein (GFP)
Microfilament
Mouse talin
Oryza sativa
Pollen
 
Issue Date2002
 
CitationActa Botanica Sinica, 2002, v. 44 n. 6, p. 642-648 [How to Cite?]
 
AbstractGreen flourescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants. Actin microfilaments could be visualized only in the late-developmental stage of the immature pollen. During this developmental stage, microfilaments, initially composed of very short fibrils, develop into a very complex and novel network that sometimes totally and sometimes partially encloses the vegetative nucleus and the spherical shaped generative cell in the central cytoplasm of the immature pollen. The behavior of the actin microfilamentous structure throughout the late-developmental stage of the immature pollen is extremely dynamic, and the likelihood of this structure in generating forces for vegetative nucleus and generative cell movement in the immature pollen has been discussed. No actin filaments were visualized in the spherical generative cells.
 
ISSN0577-7496
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorXu, SX
 
dc.contributor.authorWang, LJ
 
dc.contributor.authorQiu, ZP
 
dc.contributor.authorYe, YJ
 
dc.contributor.authorYu, XH
 
dc.date.accessioned2010-09-06T06:05:19Z
 
dc.date.available2010-09-06T06:05:19Z
 
dc.date.issued2002
 
dc.description.abstractGreen flourescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants. Actin microfilaments could be visualized only in the late-developmental stage of the immature pollen. During this developmental stage, microfilaments, initially composed of very short fibrils, develop into a very complex and novel network that sometimes totally and sometimes partially encloses the vegetative nucleus and the spherical shaped generative cell in the central cytoplasm of the immature pollen. The behavior of the actin microfilamentous structure throughout the late-developmental stage of the immature pollen is extremely dynamic, and the likelihood of this structure in generating forces for vegetative nucleus and generative cell movement in the immature pollen has been discussed. No actin filaments were visualized in the spherical generative cells.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationActa Botanica Sinica, 2002, v. 44 n. 6, p. 642-648 [How to Cite?]
 
dc.identifier.epage648
 
dc.identifier.hkuros67987
 
dc.identifier.issn0577-7496
 
dc.identifier.issue6
 
dc.identifier.scopuseid_2-s2.0-0036329819
 
dc.identifier.spage642
 
dc.identifier.urihttp://hdl.handle.net/10722/68518
 
dc.identifier.volume44
 
dc.languageeng
 
dc.publisher.placeChina
 
dc.relation.ispartofActa Botanica Sinica
 
dc.relation.referencesReferences in Scopus
 
dc.subjectActin
 
dc.subjectGreen fluorescent protein (GFP)
 
dc.subjectMicrofilament
 
dc.subjectMouse talin
 
dc.subjectOryza sativa
 
dc.subjectPollen
 
dc.titleActin visualization in living immature pollen of rice using a GFP-mouse talin fusion protein
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong