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Article: Actin visualization in living immature pollen of rice using a GFP-mouse talin fusion protein

TitleActin visualization in living immature pollen of rice using a GFP-mouse talin fusion protein
绿色荧光蛋白基因结合鼠Talin基因蛋白标记转基因水稻未成熟花粉的微丝骨架
Authors
KeywordsActin
Green fluorescent protein (GFP)
Microfilament
Mouse talin
Oryza sativa
Pollen
Issue Date2002
PublisherScience Press (科学出版社) The Journal's web site is located at http://www.jipb.net/
Citation
Acta Botanica Sinica, 2002, v. 44 n. 6, p. 642-648 How to Cite?
植物学报, 2002, v. 44 n. 6, p. 642-648 How to Cite?
AbstractGreen flourescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants. Actin microfilaments could be visualized only in the late-developmental stage of the immature pollen. During this developmental stage, microfilaments, initially composed of very short fibrils, develop into a very complex and novel network that sometimes totally and sometimes partially encloses the vegetative nucleus and the spherical shaped generative cell in the central cytoplasm of the immature pollen. The behavior of the actin microfilamentous structure throughout the late-developmental stage of the immature pollen is extremely dynamic, and the likelihood of this structure in generating forces for vegetative nucleus and generative cell movement in the immature pollen has been discussed. No actin filaments were visualized in the spherical generative cells. 利用绿色荧光蛋白基因结合鼠Talin基因表达技术及水稻转基因技术,在未成熟花粉发育期(即生殖细胞在形成后从靠壁部位移向中央部位的阶段)的水稻(Oryza sativa L.)内发现了一系列前人未曾报道过的微丝骨架的形成和多变过程.在这一发育阶段,未成熟花粉内的生殖细胞呈圆形,中央部位存有一个大液泡,大量微丝在细胞的中央胞质内形成.微丝首先在营养核的核膜表面形成两个集结中心,中心内的微丝呈短粗状.尔后,中心微丝不断延长,最终在细胞中央的胞质内形成一个非常复杂的类似多个纺锤体结合在一起的网络结构.这一网络的中间部位经常包围着营养核和生殖细胞,网络的部分微丝则与存在周缘细胞质(或称周质)的微丝网络形成连接,在连接点部位则形成一些由微丝环状组成的结构.未成熟花粉中央的微丝网络可能与营养核和生殖细胞在未成熟花粉内的运动有密切关系.
Persistent Identifierhttp://hdl.handle.net/10722/68518
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorXu, SXen_HK
dc.contributor.authorWang, LJen_HK
dc.contributor.authorQiu, ZPen_HK
dc.contributor.authorYe, YJen_HK
dc.contributor.authorYu, XHen_HK
dc.date.accessioned2010-09-06T06:05:19Z-
dc.date.available2010-09-06T06:05:19Z-
dc.date.issued2002en_HK
dc.identifier.citationActa Botanica Sinica, 2002, v. 44 n. 6, p. 642-648en_HK
dc.identifier.citation植物学报, 2002, v. 44 n. 6, p. 642-648-
dc.identifier.issn0577-7496en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68518-
dc.description.abstractGreen flourescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants. Actin microfilaments could be visualized only in the late-developmental stage of the immature pollen. During this developmental stage, microfilaments, initially composed of very short fibrils, develop into a very complex and novel network that sometimes totally and sometimes partially encloses the vegetative nucleus and the spherical shaped generative cell in the central cytoplasm of the immature pollen. The behavior of the actin microfilamentous structure throughout the late-developmental stage of the immature pollen is extremely dynamic, and the likelihood of this structure in generating forces for vegetative nucleus and generative cell movement in the immature pollen has been discussed. No actin filaments were visualized in the spherical generative cells. 利用绿色荧光蛋白基因结合鼠Talin基因表达技术及水稻转基因技术,在未成熟花粉发育期(即生殖细胞在形成后从靠壁部位移向中央部位的阶段)的水稻(Oryza sativa L.)内发现了一系列前人未曾报道过的微丝骨架的形成和多变过程.在这一发育阶段,未成熟花粉内的生殖细胞呈圆形,中央部位存有一个大液泡,大量微丝在细胞的中央胞质内形成.微丝首先在营养核的核膜表面形成两个集结中心,中心内的微丝呈短粗状.尔后,中心微丝不断延长,最终在细胞中央的胞质内形成一个非常复杂的类似多个纺锤体结合在一起的网络结构.这一网络的中间部位经常包围着营养核和生殖细胞,网络的部分微丝则与存在周缘细胞质(或称周质)的微丝网络形成连接,在连接点部位则形成一些由微丝环状组成的结构.未成熟花粉中央的微丝网络可能与营养核和生殖细胞在未成熟花粉内的运动有密切关系.en_HK
dc.languageengen_HK
dc.publisherScience Press (科学出版社) The Journal's web site is located at http://www.jipb.net/-
dc.relation.ispartofActa Botanica Sinicaen_HK
dc.relation.ispartof植物学报-
dc.subjectActinen_HK
dc.subjectGreen fluorescent protein (GFP)en_HK
dc.subjectMicrofilamenten_HK
dc.subjectMouse talinen_HK
dc.subjectOryza sativaen_HK
dc.subjectPollenen_HK
dc.subject.meshMicrofilaments (微丝)-
dc.subject.meshPollen (花粉)-
dc.subject.meshGerm Cells (生殖细胞)-
dc.subject.meshNuclear Envelope (核被膜)-
dc.titleActin visualization in living immature pollen of rice using a GFP-mouse talin fusion proteinen_HK
dc.title绿色荧光蛋白基因结合鼠Talin基因蛋白标记转基因水稻未成熟花粉的微丝骨架-
dc.typeArticleen_HK
dc.identifier.emailYe, YJ: wkyip@hkucc.hku.hken_HK
dc.identifier.authorityYe, YJ=rp00833en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3321/j.issn:1672-9072.2002.06.002-
dc.identifier.scopuseid_2-s2.0-0036329819en_HK
dc.identifier.hkuros67987en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036329819&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume44en_HK
dc.identifier.issue6en_HK
dc.identifier.spage642en_HK
dc.identifier.epage648en_HK
dc.publisher.placeBeijing (北京)en_HK
dc.identifier.scopusauthoridXu, SX=7404438821en_HK
dc.identifier.scopusauthoridWang, LJ=7409175686en_HK
dc.identifier.scopusauthoridQiu, ZP=7202941720en_HK
dc.identifier.scopusauthoridYe, YJ=7102784428en_HK
dc.identifier.scopusauthoridYu, XH=37861474600en_HK
dc.customcontrol.immutablecsl 150514-

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