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Article: Application of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Lonicerae

TitleApplication of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Lonicerae
Authors
KeywordsChlorogenic acid
Counter-current chromatography
Pharmaceutical analysis
Plant materials
Preparative chromatography
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2004, v. 1026 n. 1-2, p. 185-190 How to Cite?
AbstractChlorogenic acid, an ester formed between caffeic acid and quinic acid, is a major phenolic compound in the traditional Chinese medicinal herb Flos Lonicerae. The separation and purification of chlorogenic acid from the crude extract of Flos Lonicerae was achieved by high-speed counter-current chromatography (HSCCC). A high acid, highly polar two-phase solvent system containing n-butanol-acetic acid-water (4:1:5) was run on a preparative scale. The upper phase was used as the mobile phase in the head to tail elution mode. A 300-mg quantity of the crude extract containing 5.97% chlorogenic acid was loaded on a 342-ml HSCCC column. Double separations were performed with the same solvent system yielding 16.9mg chlorogenic acid at 94.8% purity with approximately 90% recovery. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68516
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, HTen_HK
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:05:17Z-
dc.date.available2010-09-06T06:05:17Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Chromatography A, 2004, v. 1026 n. 1-2, p. 185-190en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68516-
dc.description.abstractChlorogenic acid, an ester formed between caffeic acid and quinic acid, is a major phenolic compound in the traditional Chinese medicinal herb Flos Lonicerae. The separation and purification of chlorogenic acid from the crude extract of Flos Lonicerae was achieved by high-speed counter-current chromatography (HSCCC). A high acid, highly polar two-phase solvent system containing n-butanol-acetic acid-water (4:1:5) was run on a preparative scale. The upper phase was used as the mobile phase in the head to tail elution mode. A 300-mg quantity of the crude extract containing 5.97% chlorogenic acid was loaded on a 342-ml HSCCC column. Double separations were performed with the same solvent system yielding 16.9mg chlorogenic acid at 94.8% purity with approximately 90% recovery. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectChlorogenic aciden_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectPharmaceutical analysisen_HK
dc.subjectPlant materialsen_HK
dc.subjectPreparative chromatographyen_HK
dc.titleApplication of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Loniceraeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1026&issue=1-2&spage=185&epage=190&date=2004&atitle=Application+of+preparative+high-speed+counter-current+chromatography+for+separation+of+chlorogenic+acid+from+Flos+Loniceraeen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2003.11.004en_HK
dc.identifier.pmid14763745-
dc.identifier.scopuseid_2-s2.0-0347360203en_HK
dc.identifier.hkuros90919en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0347360203&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1026en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage185en_HK
dc.identifier.epage190en_HK
dc.identifier.isiWOS:000188200300021-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLu, HT=7404843255en_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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