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Article: Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions

TitleDifferential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions
Authors
KeywordsAuxin
Differential expression
Ethylene
Ethylene receptors
Rice
Issue Date2004
PublisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/
Citation
Journal Of Experimental Botany, 2004, v. 55 n. 397, p. 547-556 How to Cite?
AbstractFive ethylene receptor genes, OS-ERS1, OS-ERS2, OS-ETR2, OS-ETR3, and OS-ETR4 were isolated and characterized from rice. The genomic structure of OS-ERS1 and OS-ERS2 revealed that the introns within the coding sequences occurred in conserved positions to those of At-ETR1 and At-ERS1, whereas each of the OS-ETR2, OS-ETR3, and OS-ETR4 genes contained 1 intron within its coding region located at a position equivalent to those of At-ERS2, At-ETR2, and At-EIN4. Deduced amino acid sequences of OS-ERS1, OS-ERS2, OS-ETR2, OS-ETR3, and OS-ETR4 showed that they exhibited significant homology to the prokaryotic two-component signal transducer and a wide range of ethylene receptors in a variety of plant species. Northern analysis revealed that the level of OS-ETR2 mRNA was markedly elevated either by the exogenous application of IAA or by ethylene treatment in young etiolated rice seedlings, whereas the OS-ERS1 transcript level was only slightly induced under the same experimental conditions. Pretreatment with silver prevented IAA-induced and ethylene-induced accumulation of both mRNAs (OS-ERS1 and OS-ETR2). However, the abundance of OS-ERS2 mRNA was shown to be down-regulated by both IAA and ethylene treatments, indicating that it was not positively regulated by ethylene. Analysis of the expression of the three ethylene receptor genes in different tissues of rice has unravelled their corresponding tissue-specificity in which OS-ERS1 was constitutively expressed in considerable amounts in all tissues studied, while OS-ERS2 and OS-ETR2 exhibited differential expression patterns in different tissues of rice. Moreover, higher levels of these three mRNAs were commonly observed in anthers when compared with their corresponding levels in other tissues, suggesting the important role played by ethylene involved in the regulation of pollen development in rice. Among the five ethylene receptor genes, the expression levels of both OS-ETR3 and OS-ETR4 were too low to be detected by the northern blot analysis. Results from RT-PCR illustrated that both mRNAs were present in young green rice seedlings and anthers.
Persistent Identifierhttp://hdl.handle.net/10722/68495
ISSN
2015 Impact Factor: 5.677
2015 SCImago Journal Rankings: 2.798
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYau, CPen_HK
dc.contributor.authorWang, Len_HK
dc.contributor.authorYu, Men_HK
dc.contributor.authorZee, SYen_HK
dc.contributor.authorYip, WKen_HK
dc.date.accessioned2010-09-06T06:05:07Z-
dc.date.available2010-09-06T06:05:07Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Experimental Botany, 2004, v. 55 n. 397, p. 547-556en_HK
dc.identifier.issn0022-0957en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68495-
dc.description.abstractFive ethylene receptor genes, OS-ERS1, OS-ERS2, OS-ETR2, OS-ETR3, and OS-ETR4 were isolated and characterized from rice. The genomic structure of OS-ERS1 and OS-ERS2 revealed that the introns within the coding sequences occurred in conserved positions to those of At-ETR1 and At-ERS1, whereas each of the OS-ETR2, OS-ETR3, and OS-ETR4 genes contained 1 intron within its coding region located at a position equivalent to those of At-ERS2, At-ETR2, and At-EIN4. Deduced amino acid sequences of OS-ERS1, OS-ERS2, OS-ETR2, OS-ETR3, and OS-ETR4 showed that they exhibited significant homology to the prokaryotic two-component signal transducer and a wide range of ethylene receptors in a variety of plant species. Northern analysis revealed that the level of OS-ETR2 mRNA was markedly elevated either by the exogenous application of IAA or by ethylene treatment in young etiolated rice seedlings, whereas the OS-ERS1 transcript level was only slightly induced under the same experimental conditions. Pretreatment with silver prevented IAA-induced and ethylene-induced accumulation of both mRNAs (OS-ERS1 and OS-ETR2). However, the abundance of OS-ERS2 mRNA was shown to be down-regulated by both IAA and ethylene treatments, indicating that it was not positively regulated by ethylene. Analysis of the expression of the three ethylene receptor genes in different tissues of rice has unravelled their corresponding tissue-specificity in which OS-ERS1 was constitutively expressed in considerable amounts in all tissues studied, while OS-ERS2 and OS-ETR2 exhibited differential expression patterns in different tissues of rice. Moreover, higher levels of these three mRNAs were commonly observed in anthers when compared with their corresponding levels in other tissues, suggesting the important role played by ethylene involved in the regulation of pollen development in rice. Among the five ethylene receptor genes, the expression levels of both OS-ETR3 and OS-ETR4 were too low to be detected by the northern blot analysis. Results from RT-PCR illustrated that both mRNAs were present in young green rice seedlings and anthers.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Experimental Botanyen_HK
dc.rightsJournal of Experimental Botany. Copyright © Oxford University Press.en_HK
dc.subjectAuxinen_HK
dc.subjectDifferential expressionen_HK
dc.subjectEthyleneen_HK
dc.subjectEthylene receptorsen_HK
dc.subjectRiceen_HK
dc.titleDifferential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-0957&volume=55&issue=397&spage=547&epage=556&date=2004&atitle=Differential+expression+of+three+genes+encoding+an+ethylene+receptor+in+rice+during+development,+and+in+response+to+indole-3-acetic+acid+and+silver+ionsen_HK
dc.identifier.emailYip, WK: wkyip@hkucc.hku.hken_HK
dc.identifier.authorityYip, WK=rp00833en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/jxb/erh055en_HK
dc.identifier.pmid14754915-
dc.identifier.scopuseid_2-s2.0-1542440028en_HK
dc.identifier.hkuros87937en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542440028&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume55en_HK
dc.identifier.issue397en_HK
dc.identifier.spage547en_HK
dc.identifier.epage556en_HK
dc.identifier.isiWOS:000220073400001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYau, CP=7007038455en_HK
dc.identifier.scopusauthoridWang, L=7409175686en_HK
dc.identifier.scopusauthoridYu, M=7404272859en_HK
dc.identifier.scopusauthoridZee, SY=7004001733en_HK
dc.identifier.scopusauthoridYip, WK=7102784428en_HK

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