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Article: Preparative isolation and purification of six diterpenoids from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography

TitlePreparative isolation and purification of six diterpenoids from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography
Authors
KeywordsCounter-current chromatography
Pharmaceutical analysis
Plant materials
Salvia miltiorrhiza
Tanshinones
Terpenoids
Issue Date2001
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2001, v. 925 n. 1-2, p. 109-114 How to Cite?
AbstractA high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of six diterpenoids, dihydrotanshinone I, cryptotanshinone, methylenetanshiquinone, tanshinone I, tanshinone IIA and danshenxinkun B from the Chinese medicinal plant Salvia miltiorrhiza Bunge. The crude diterpenoids were obtained by extraction with ethanol-n-hexane (1:1, v/v) from S. miltiorrhiza Bunge. Preparative HSCCC with the two-phase solvent systems A composed of n-hexane-ethanol-water (10:5.5:4.5, v/v) and B composed of n-hexane-ethanol-water (10:7:3, v/v) was successfully performed in a stepwise elution yielding six relatively pure diterpenoids from 300 mg of the crude extract in a single run. The purities of dihydrotanshinone I, cryptotanshinone, methylenetanshiquinone, tanshinone I, tanshinone IIA and danshenxinkun B were 88.1, 98.8, 97.6, 93.5, 96.8 and 94.3%, respectively. Copyright © 2001 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/68494
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:05:06Z-
dc.date.available2010-09-06T06:05:06Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Chromatography A, 2001, v. 925 n. 1-2, p. 109-114en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68494-
dc.description.abstractA high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of six diterpenoids, dihydrotanshinone I, cryptotanshinone, methylenetanshiquinone, tanshinone I, tanshinone IIA and danshenxinkun B from the Chinese medicinal plant Salvia miltiorrhiza Bunge. The crude diterpenoids were obtained by extraction with ethanol-n-hexane (1:1, v/v) from S. miltiorrhiza Bunge. Preparative HSCCC with the two-phase solvent systems A composed of n-hexane-ethanol-water (10:5.5:4.5, v/v) and B composed of n-hexane-ethanol-water (10:7:3, v/v) was successfully performed in a stepwise elution yielding six relatively pure diterpenoids from 300 mg of the crude extract in a single run. The purities of dihydrotanshinone I, cryptotanshinone, methylenetanshiquinone, tanshinone I, tanshinone IIA and danshenxinkun B were 88.1, 98.8, 97.6, 93.5, 96.8 and 94.3%, respectively. Copyright © 2001 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectPharmaceutical analysisen_HK
dc.subjectPlant materialsen_HK
dc.subjectSalvia miltiorrhizaen_HK
dc.subjectTanshinonesen_HK
dc.subjectTerpenoidsen_HK
dc.titlePreparative isolation and purification of six diterpenoids from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatographyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=925&spage=109&epage=114&date=2001&atitle=Preparative+isolation+and+purification+of+six+diterpenoids+from+the+Chinese+medicinal+plant+Salvia+miltiorrhiza+by+high-speed+counter-current+chromatographyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0021-9673(01)01026-3en_HK
dc.identifier.pmid11519796-
dc.identifier.scopuseid_2-s2.0-0035800697en_HK
dc.identifier.hkuros72664en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035800697&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume925en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage109en_HK
dc.identifier.epage114en_HK
dc.identifier.isiWOS:000170432100010-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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