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Article: Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography

TitleIsolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography
Authors
KeywordsBaicalein
Counter-current chromatography
Oroxylin A
Plant materials
Preparative chromatography
Scutellaria baicalensis
Wogonin
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2005, v. 1074 n. 1-2, p. 107-110 How to Cite?
AbstractThe medicinal plant Scutellaria baicalensis Georgi has been used widely in traditional Chinese medicine for anti-inflammation, anticancer, antiviral and antibacterial infections, reducing the total cholesterol level and decreasing blood pressures. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of three bioactive flavonoids, namely, baicalein, wogonin and oroxylin A, from S. baicalensis Georgi. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-n-butanol-water (1:1:8:10, v/v/v/v) was successfully performed by increasing the flow-rate of the mobile phase stepwise from 1.0 to 2.0 ml min-1 after 4 h. The components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 144.8 mg of baicalein at 95.7% purity, 50.2 mg of wogonin at 98.5% purity, and 12.4 mg of oroxylin A at 93.2% purity from 500 mg of the crude extract in a one-step separation. The recoveries of baicalein, wogonin and oroxylin A were 92.7%, 91.6% and 92.5%, respectively. © 2005 Published by Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/68491
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:05:05Z-
dc.date.available2010-09-06T06:05:05Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Chromatography A, 2005, v. 1074 n. 1-2, p. 107-110en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68491-
dc.description.abstractThe medicinal plant Scutellaria baicalensis Georgi has been used widely in traditional Chinese medicine for anti-inflammation, anticancer, antiviral and antibacterial infections, reducing the total cholesterol level and decreasing blood pressures. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of three bioactive flavonoids, namely, baicalein, wogonin and oroxylin A, from S. baicalensis Georgi. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-n-butanol-water (1:1:8:10, v/v/v/v) was successfully performed by increasing the flow-rate of the mobile phase stepwise from 1.0 to 2.0 ml min-1 after 4 h. The components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 144.8 mg of baicalein at 95.7% purity, 50.2 mg of wogonin at 98.5% purity, and 12.4 mg of oroxylin A at 93.2% purity from 500 mg of the crude extract in a one-step separation. The recoveries of baicalein, wogonin and oroxylin A were 92.7%, 91.6% and 92.5%, respectively. © 2005 Published by Elsevier B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectBaicaleinen_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectOroxylin Aen_HK
dc.subjectPlant materialsen_HK
dc.subjectPreparative chromatographyen_HK
dc.subjectScutellaria baicalensisen_HK
dc.subjectWogoninen_HK
dc.titleIsolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatographyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1074&spage=107&epage=110&date=2005&atitle=Isolation+and+purification+of+baicalein,+wogonin+and+oroxylin+A+from+the+medicinal+plant+Scutellaria+baicalensis+by+high-speed-counter-current+chromatographyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2005.03.088en_HK
dc.identifier.pmid15941045-
dc.identifier.scopuseid_2-s2.0-18144407267en_HK
dc.identifier.hkuros104900en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18144407267&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1074en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage107en_HK
dc.identifier.epage110en_HK
dc.identifier.isiWOS:000229192500014-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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