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- Publisher Website: 10.1016/j.chroma.2004.09.005
- Scopus: eid_2-s2.0-4744352548
- PMID: 15527143
- WOS: WOS:000224501000029
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Article: Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography
Title | Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography |
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Authors | |
Keywords | Counter-current chromatography Gastrodia elata Gastrodin Plant materials Preparative chromatography |
Issue Date | 2004 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma |
Citation | Journal Of Chromatography A, 2004, v. 1052 n. 1-2, p. 229-232 How to Cite? |
Abstract | Gastrodia elata Blume is a famous Chinese medicinal plant, which has been widely used for the treatment of rheumatism, epilepsy, paralysis, hemiplegia, lumbago, headache and vertigo. High-speed counter-current chromatography was successfully used for the first time for the preparative isolation and purification of the bioactive component gastrodin from G. elata Blume. The crude gastrodin was obtained by extraction with ethanol from the dried roots of G. elata Blume under sonication. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-butanol-ethyl acetate-water (2:3:5, v/v/v) was successfully performed yielding 48 mg gastrodin at 96% purity from 500 mg of the crude extract (10.3% gastrodin) with the recovery of approximately 90% in a one-step separation. © 2004 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/68489 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.717 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, HB | en_HK |
dc.contributor.author | Chen, F | en_HK |
dc.date.accessioned | 2010-09-06T06:05:03Z | - |
dc.date.available | 2010-09-06T06:05:03Z | - |
dc.date.issued | 2004 | en_HK |
dc.identifier.citation | Journal Of Chromatography A, 2004, v. 1052 n. 1-2, p. 229-232 | en_HK |
dc.identifier.issn | 0021-9673 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/68489 | - |
dc.description.abstract | Gastrodia elata Blume is a famous Chinese medicinal plant, which has been widely used for the treatment of rheumatism, epilepsy, paralysis, hemiplegia, lumbago, headache and vertigo. High-speed counter-current chromatography was successfully used for the first time for the preparative isolation and purification of the bioactive component gastrodin from G. elata Blume. The crude gastrodin was obtained by extraction with ethanol from the dried roots of G. elata Blume under sonication. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-butanol-ethyl acetate-water (2:3:5, v/v/v) was successfully performed yielding 48 mg gastrodin at 96% purity from 500 mg of the crude extract (10.3% gastrodin) with the recovery of approximately 90% in a one-step separation. © 2004 Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma | en_HK |
dc.relation.ispartof | Journal of Chromatography A | en_HK |
dc.rights | Journal of Chromatography A. Copyright © Elsevier BV. | en_HK |
dc.subject | Counter-current chromatography | en_HK |
dc.subject | Gastrodia elata | en_HK |
dc.subject | Gastrodin | en_HK |
dc.subject | Plant materials | en_HK |
dc.subject | Preparative chromatography | en_HK |
dc.title | Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1052&issue=1-2&spage=229&epage=232&date=2004&atitle=Preparative+isolation+and+purification+of+gastrodin+from+the+Chinese+medicinal+plant+Gastrodia+elata+by+high-speed+counter-current+chromatography | en_HK |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_HK |
dc.identifier.authority | Chen, F=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.chroma.2004.09.005 | en_HK |
dc.identifier.pmid | 15527143 | - |
dc.identifier.scopus | eid_2-s2.0-4744352548 | en_HK |
dc.identifier.hkuros | 105004 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-4744352548&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 1052 | en_HK |
dc.identifier.issue | 1-2 | en_HK |
dc.identifier.spage | 229 | en_HK |
dc.identifier.epage | 232 | en_HK |
dc.identifier.isi | WOS:000224501000029 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_HK |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_HK |
dc.identifier.issnl | 0021-9673 | - |