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Article: A proteinase inhibitor II of Solanum americanum is expressed in phloem

TitleA proteinase inhibitor II of Solanum americanum is expressed in phloem
Authors
KeywordsBlack nightshade
Companion cell
Immunolocalization
In situ hybridization
Sieve element
Sieve-area pore
Issue Date2001
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412
Citation
Plant Molecular Biology, 2001, v. 47 n. 6, p. 727-738 How to Cite?
AbstractAlthough proteinase inhibitor proteins are known to confer insect resistance in transgenic plants, their endogenous roles remain undefined. Here, we describe the expression of a proteinase inhibitor II (PIN2) protein from Solanum americanum in phloem of stems, roots and leaves suggesting a novel endogenous role for PIN2 in phloem. The phloem consists of parenchyma cells, sieve elements (SE), and companion cells (CC) which are in close association with SE. We isolated two cDNAs encoding PIN2, SaPIN2a and SaPIN2b, from a S. americanum cDNA library using a tomato PIN2 cDNA as hybridization probe. SaPIN2a shows 73.6% identity to SaPIN2b. Southern blot analysis confirmed that two genes occur in S. americanum. Northern blot analysis showed that both are wound-inducible and are expressed in flowers. Unlike SaPIN2b and other previously characterized plant PIN2 proteins, SaPIN2a is abundantly expressed in stems. In situ hybridization studies on stem sections showed that SaPIN2a mRNA is expressed in CC and some SE, likely the immature developing SE, of external and internal phloem. Western blot analysis using SaPIN2a-specific antibodies showed SaPIN2a accumulation in stems, leaf midribs and fruits. Immunohistochemical localization, using these antibodies, revealed SaPIN2a expression in external and internal phloem of stem. Immunoelectron microscopy of stem, root and leaf sections further localized SaPIN2a to the CC and predominantly to the SE, particularly the parietal cytoplasm adjacent to the cell wall, the lumen and the sieve-area pores. These results suggest that, other than a possible role in plant defense, SaPIN2a could be involved in regulating proteolysis in the SE.
Persistent Identifierhttp://hdl.handle.net/10722/68479
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 1.915
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, ZFen_HK
dc.contributor.authorQi, WQen_HK
dc.contributor.authorOuyang, XZen_HK
dc.contributor.authorYeung, Een_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2010-09-06T06:04:58Z-
dc.date.available2010-09-06T06:04:58Z-
dc.date.issued2001en_HK
dc.identifier.citationPlant Molecular Biology, 2001, v. 47 n. 6, p. 727-738en_HK
dc.identifier.issn0167-4412en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68479-
dc.description.abstractAlthough proteinase inhibitor proteins are known to confer insect resistance in transgenic plants, their endogenous roles remain undefined. Here, we describe the expression of a proteinase inhibitor II (PIN2) protein from Solanum americanum in phloem of stems, roots and leaves suggesting a novel endogenous role for PIN2 in phloem. The phloem consists of parenchyma cells, sieve elements (SE), and companion cells (CC) which are in close association with SE. We isolated two cDNAs encoding PIN2, SaPIN2a and SaPIN2b, from a S. americanum cDNA library using a tomato PIN2 cDNA as hybridization probe. SaPIN2a shows 73.6% identity to SaPIN2b. Southern blot analysis confirmed that two genes occur in S. americanum. Northern blot analysis showed that both are wound-inducible and are expressed in flowers. Unlike SaPIN2b and other previously characterized plant PIN2 proteins, SaPIN2a is abundantly expressed in stems. In situ hybridization studies on stem sections showed that SaPIN2a mRNA is expressed in CC and some SE, likely the immature developing SE, of external and internal phloem. Western blot analysis using SaPIN2a-specific antibodies showed SaPIN2a accumulation in stems, leaf midribs and fruits. Immunohistochemical localization, using these antibodies, revealed SaPIN2a expression in external and internal phloem of stem. Immunoelectron microscopy of stem, root and leaf sections further localized SaPIN2a to the CC and predominantly to the SE, particularly the parietal cytoplasm adjacent to the cell wall, the lumen and the sieve-area pores. These results suggest that, other than a possible role in plant defense, SaPIN2a could be involved in regulating proteolysis in the SE.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412en_HK
dc.relation.ispartofPlant Molecular Biologyen_HK
dc.subjectBlack nightshadeen_HK
dc.subjectCompanion cellen_HK
dc.subjectImmunolocalizationen_HK
dc.subjectIn situ hybridizationen_HK
dc.subjectSieve elementen_HK
dc.subjectSieve-area poreen_HK
dc.titleA proteinase inhibitor II of Solanum americanum is expressed in phloemen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-4412&volume=47&spage=727&epage=738&date=2001&atitle=A+proteinase+inhibitor+II+of+Solanum+americanum+is+expressed+in+phloemen_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1013623628857en_HK
dc.identifier.pmid11785934-
dc.identifier.scopuseid_2-s2.0-0035543848en_HK
dc.identifier.hkuros65879en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035543848&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue6en_HK
dc.identifier.spage727en_HK
dc.identifier.epage738en_HK
dc.identifier.isiWOS:000173046300004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridXu, ZF=7405425506en_HK
dc.identifier.scopusauthoridQi, WQ=55119073400en_HK
dc.identifier.scopusauthoridOuyang, XZ=7102723642en_HK
dc.identifier.scopusauthoridYeung, E=7101971768en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK

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