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Article: Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture

TitleEmployment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture
Authors
KeywordsAstaxanthin
Chlorella zofingiensis
Heterotrophic culture
Oxidative stress
Reactive oxygen species
Secondary carotenoids
Issue Date2005
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio
Citation
Process Biochemistry, 2005, v. 40 n. 11, p. 3491-3496 How to Cite?
AbstractThe advantages of using heterotrophic cultivation for commercial production of the high-value ketocarotenoid astaxanthin by microalgae have been emphasized in recent years [Chen F. High cell density culture of microalgae in heterotrophic growth. Trends Biotechnol 1996;14:421-5; Chen F, Chen H, Gong X. Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions. Bioresour Technol 1997;62:19-24; Ip PF, Chen F. Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark. Process Biochem 2005;40:733-8]. In a heterotrophic culture where light is not provided, the employment of oxidative stress is essential for promoting the formation of secondary carotenoids including astaxanthin. In the present study, oxidative treatment imposed by chemically generated reactive oxygen species (ROS) including the hydroxyl radicals (OH) and singlet oxygen ( 1O2) for inducing carotenogenesis and astaxanthin production by C. zofingiensis in heterotrophic culture was investigated. OH stimulated secondary carotenoids accumulation significantly, whereas 1O2 did not. The addition of 0.1 mM hydrogen peroxide to the culture leading to the generation of OH enhanced the yield of astaxanthin from 9.9 mg l-1 to 12.58 mg l-1. These results indicate that the application of a suitable type and dosage of ROS is potentially useful for mass production of astaxanthin by C. zofingiensis in heterotrophic culture. © 2005 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68478
ISSN
2015 Impact Factor: 2.529
2015 SCImago Journal Rankings: 0.937
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorIp, PFen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:58Z-
dc.date.available2010-09-06T06:04:58Z-
dc.date.issued2005en_HK
dc.identifier.citationProcess Biochemistry, 2005, v. 40 n. 11, p. 3491-3496en_HK
dc.identifier.issn1359-5113en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68478-
dc.description.abstractThe advantages of using heterotrophic cultivation for commercial production of the high-value ketocarotenoid astaxanthin by microalgae have been emphasized in recent years [Chen F. High cell density culture of microalgae in heterotrophic growth. Trends Biotechnol 1996;14:421-5; Chen F, Chen H, Gong X. Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions. Bioresour Technol 1997;62:19-24; Ip PF, Chen F. Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark. Process Biochem 2005;40:733-8]. In a heterotrophic culture where light is not provided, the employment of oxidative stress is essential for promoting the formation of secondary carotenoids including astaxanthin. In the present study, oxidative treatment imposed by chemically generated reactive oxygen species (ROS) including the hydroxyl radicals (OH) and singlet oxygen ( 1O2) for inducing carotenogenesis and astaxanthin production by C. zofingiensis in heterotrophic culture was investigated. OH stimulated secondary carotenoids accumulation significantly, whereas 1O2 did not. The addition of 0.1 mM hydrogen peroxide to the culture leading to the generation of OH enhanced the yield of astaxanthin from 9.9 mg l-1 to 12.58 mg l-1. These results indicate that the application of a suitable type and dosage of ROS is potentially useful for mass production of astaxanthin by C. zofingiensis in heterotrophic culture. © 2005 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbioen_HK
dc.relation.ispartofProcess Biochemistryen_HK
dc.rightsProcess Biochemistry. Copyright © Elsevier Ltd.en_HK
dc.subjectAstaxanthinen_HK
dc.subjectChlorella zofingiensisen_HK
dc.subjectHeterotrophic cultureen_HK
dc.subjectOxidative stressen_HK
dc.subjectReactive oxygen speciesen_HK
dc.subjectSecondary carotenoidsen_HK
dc.titleEmployment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic cultureen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1359-5113&volume=40&spage=3491&epage=3496&date=2005&atitle=Employment+of+reactive+oxygen+species+to+enhance+astaxanthin+formation+in+Chlorella+zofingiensis+in+heterotrophic+cultureen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.procbio.2005.02.014en_HK
dc.identifier.scopuseid_2-s2.0-24944561909en_HK
dc.identifier.hkuros122509en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-24944561909&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue11en_HK
dc.identifier.spage3491en_HK
dc.identifier.epage3496en_HK
dc.identifier.isiWOS:000232393700015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridIp, PF=7003622680en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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