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Article: Preparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by high-speed counter-current chromatography

TitlePreparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by high-speed counter-current chromatography
Authors
KeywordsCounter-current chromatography
Forsythia suspensa
Phillyrin
Plant materials
Preparative chromatography
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2005, v. 1083 n. 1-2, p. 102-105 How to Cite?
AbstractForsythia suspensa (Thunb.) Vahl. has been used widely in traditional medicines to treat gonorrhea, erysipelas, inflammation, pyrexia and ulcer. It has also shown antioxidant activity, as well as antibacterial, antiviral, choleretic and antiemetic effects. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of the bioactive molecule phillyrin from F. suspensa (Thunb.) Vahl. The crude phillyrin was obtained by extraction with 50% ethanol from the dried fruits of F. suspensa (Thunb.) Vahl. under sonication. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (1:9:1:9, v/v/v/v) was successfully performed, and the components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 5.6 mg phillyrin at 98.6% purity from 500 mg of the crude extract (1.2% phillyrin) with the recovery of 92% in a one-step separation. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68470
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:53Z-
dc.date.available2010-09-06T06:04:53Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Chromatography A, 2005, v. 1083 n. 1-2, p. 102-105en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68470-
dc.description.abstractForsythia suspensa (Thunb.) Vahl. has been used widely in traditional medicines to treat gonorrhea, erysipelas, inflammation, pyrexia and ulcer. It has also shown antioxidant activity, as well as antibacterial, antiviral, choleretic and antiemetic effects. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of the bioactive molecule phillyrin from F. suspensa (Thunb.) Vahl. The crude phillyrin was obtained by extraction with 50% ethanol from the dried fruits of F. suspensa (Thunb.) Vahl. under sonication. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (1:9:1:9, v/v/v/v) was successfully performed, and the components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 5.6 mg phillyrin at 98.6% purity from 500 mg of the crude extract (1.2% phillyrin) with the recovery of 92% in a one-step separation. © 2005 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectForsythia suspensaen_HK
dc.subjectPhillyrinen_HK
dc.subjectPlant materialsen_HK
dc.subjectPreparative chromatographyen_HK
dc.titlePreparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by high-speed counter-current chromatographyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1083&spage=102&epage=105&date=2005&atitle=Preparative+isolation+and+purification+of+phillyrin+from+the+medicinal+plant+Forsythia+suspensa+by+high-speed+counter-current+chromatographyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2005.06.025en_HK
dc.identifier.pmid16078694-
dc.identifier.scopuseid_2-s2.0-21544458363en_HK
dc.identifier.hkuros122505en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-21544458363&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1083en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage102en_HK
dc.identifier.epage105en_HK
dc.identifier.isiWOS:000230538400014-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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