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- Publisher Website: 10.1016/j.chroma.2005.06.025
- Scopus: eid_2-s2.0-21544458363
- PMID: 16078694
- WOS: WOS:000230538400014
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Article: Preparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by high-speed counter-current chromatography
Title | Preparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by high-speed counter-current chromatography |
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Authors | |
Keywords | Counter-current chromatography Forsythia suspensa Phillyrin Plant materials Preparative chromatography |
Issue Date | 2005 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma |
Citation | Journal Of Chromatography A, 2005, v. 1083 n. 1-2, p. 102-105 How to Cite? |
Abstract | Forsythia suspensa (Thunb.) Vahl. has been used widely in traditional medicines to treat gonorrhea, erysipelas, inflammation, pyrexia and ulcer. It has also shown antioxidant activity, as well as antibacterial, antiviral, choleretic and antiemetic effects. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of the bioactive molecule phillyrin from F. suspensa (Thunb.) Vahl. The crude phillyrin was obtained by extraction with 50% ethanol from the dried fruits of F. suspensa (Thunb.) Vahl. under sonication. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (1:9:1:9, v/v/v/v) was successfully performed, and the components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 5.6 mg phillyrin at 98.6% purity from 500 mg of the crude extract (1.2% phillyrin) with the recovery of 92% in a one-step separation. © 2005 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/68470 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.717 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, HB | en_HK |
dc.contributor.author | Chen, F | en_HK |
dc.date.accessioned | 2010-09-06T06:04:53Z | - |
dc.date.available | 2010-09-06T06:04:53Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | Journal Of Chromatography A, 2005, v. 1083 n. 1-2, p. 102-105 | en_HK |
dc.identifier.issn | 0021-9673 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/68470 | - |
dc.description.abstract | Forsythia suspensa (Thunb.) Vahl. has been used widely in traditional medicines to treat gonorrhea, erysipelas, inflammation, pyrexia and ulcer. It has also shown antioxidant activity, as well as antibacterial, antiviral, choleretic and antiemetic effects. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of the bioactive molecule phillyrin from F. suspensa (Thunb.) Vahl. The crude phillyrin was obtained by extraction with 50% ethanol from the dried fruits of F. suspensa (Thunb.) Vahl. under sonication. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (1:9:1:9, v/v/v/v) was successfully performed, and the components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 5.6 mg phillyrin at 98.6% purity from 500 mg of the crude extract (1.2% phillyrin) with the recovery of 92% in a one-step separation. © 2005 Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma | en_HK |
dc.relation.ispartof | Journal of Chromatography A | en_HK |
dc.rights | Journal of Chromatography A. Copyright © Elsevier BV. | en_HK |
dc.subject | Counter-current chromatography | en_HK |
dc.subject | Forsythia suspensa | en_HK |
dc.subject | Phillyrin | en_HK |
dc.subject | Plant materials | en_HK |
dc.subject | Preparative chromatography | en_HK |
dc.title | Preparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by high-speed counter-current chromatography | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1083&spage=102&epage=105&date=2005&atitle=Preparative+isolation+and+purification+of+phillyrin+from+the+medicinal+plant+Forsythia+suspensa+by+high-speed+counter-current+chromatography | en_HK |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_HK |
dc.identifier.authority | Chen, F=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.chroma.2005.06.025 | en_HK |
dc.identifier.pmid | 16078694 | - |
dc.identifier.scopus | eid_2-s2.0-21544458363 | en_HK |
dc.identifier.hkuros | 122505 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-21544458363&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 1083 | en_HK |
dc.identifier.issue | 1-2 | en_HK |
dc.identifier.spage | 102 | en_HK |
dc.identifier.epage | 105 | en_HK |
dc.identifier.isi | WOS:000230538400014 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_HK |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_HK |
dc.identifier.issnl | 0021-9673 | - |