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Article: In vitro studies of endophytic fungi from Tripterygium wilfordii with anti-proliferative activity on human peripheral blood mononuclear cells

TitleIn vitro studies of endophytic fungi from Tripterygium wilfordii with anti-proliferative activity on human peripheral blood mononuclear cells
Authors
KeywordsAnti-proliferative activity
Cytotoxicity
Endophytic fungi
Peripheral blood mononuclear cells
Issue Date2004
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2004, v. 94 n. 2-3, p. 295-300 How to Cite?
AbstractAn array of endophytic fungi which habitat in a Chinese medicinal plant, Tripterygium wilfordii Hook. f., (TWHf) were isolated and tested for their suppressive activity on phytohemaglutinin (PHA) stimulated proliferation of human peripheral blood mononuclear cells (PBMC). Out of the 343 isolates, representing 60 different morphotypes, were screened. Amongst the screened fungi, only 15 isolates showed anti-proliferative activity. Ethyl acetate extracts of Pestalotiopsis leucothës, Mucor sp. Verticillium sp. or Pestalotiopsis disseminata, in particular, significantly inhibited the proliferation at doses between 0.12 and 500 μg/ml (stimulation index (S.I.) ranges 0.01-0.70) (P < 0.001-0.05). IC 50 values of these four fungal extracts were between 0.75-0.80 ± 0.12 μg/ml. Trypan blue exclusion test and visual examination of cell morphology demonstrate that the anti-proliferative effect of these extracts was not a sequential consequence of their cytotoxicity. Nevertheless, fungal extracts of Acremonium sp. A and C, Pestalotiopsis suffocata, Morphotype sp. 4 and 5 show a much higher cytotoxic effects on PBMC. Our results indicate that some fungal extracts contain significant amount of immunomodulatory principles. © 2004 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68463
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKumar, DSSen_HK
dc.contributor.authorCheung, HYen_HK
dc.contributor.authorLau, CSen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorHyde, KDen_HK
dc.date.accessioned2010-09-06T06:04:50Z-
dc.date.available2010-09-06T06:04:50Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Ethnopharmacology, 2004, v. 94 n. 2-3, p. 295-300en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68463-
dc.description.abstractAn array of endophytic fungi which habitat in a Chinese medicinal plant, Tripterygium wilfordii Hook. f., (TWHf) were isolated and tested for their suppressive activity on phytohemaglutinin (PHA) stimulated proliferation of human peripheral blood mononuclear cells (PBMC). Out of the 343 isolates, representing 60 different morphotypes, were screened. Amongst the screened fungi, only 15 isolates showed anti-proliferative activity. Ethyl acetate extracts of Pestalotiopsis leucothës, Mucor sp. Verticillium sp. or Pestalotiopsis disseminata, in particular, significantly inhibited the proliferation at doses between 0.12 and 500 μg/ml (stimulation index (S.I.) ranges 0.01-0.70) (P < 0.001-0.05). IC 50 values of these four fungal extracts were between 0.75-0.80 ± 0.12 μg/ml. Trypan blue exclusion test and visual examination of cell morphology demonstrate that the anti-proliferative effect of these extracts was not a sequential consequence of their cytotoxicity. Nevertheless, fungal extracts of Acremonium sp. A and C, Pestalotiopsis suffocata, Morphotype sp. 4 and 5 show a much higher cytotoxic effects on PBMC. Our results indicate that some fungal extracts contain significant amount of immunomodulatory principles. © 2004 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.rightsJournal of Ethnopharmacology. Copyright © Elsevier Ireland Ltd.en_HK
dc.subjectAnti-proliferative activityen_HK
dc.subjectCytotoxicityen_HK
dc.subjectEndophytic fungien_HK
dc.subjectPeripheral blood mononuclear cellsen_HK
dc.subject.meshAdulten_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFungi - isolation & purificationen_HK
dc.subject.meshGrowth Inhibitors - isolation & purification - pharmacologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLeukocytes, Mononuclear - cytology - drug effects - physiologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshPlant Extracts - isolation & purification - pharmacologyen_HK
dc.subject.meshPlant Structuresen_HK
dc.subject.meshTripterygiumen_HK
dc.titleIn vitro studies of endophytic fungi from Tripterygium wilfordii with anti-proliferative activity on human peripheral blood mononuclear cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-8741&volume=94&spage=295&epage=300&date=2004&atitle=In+vitro+studies+of+endophytic+fungi+from+Tripterygium+wilfordii+with+anti-proliferative+activity+on+human+peripheral+blood+mononuclear+cellsen_HK
dc.identifier.emailLau, CS: cslau@hku.hken_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityLau, CS=rp01348en_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jep.2004.05.019en_HK
dc.identifier.pmid15325734-
dc.identifier.scopuseid_2-s2.0-4344600731en_HK
dc.identifier.hkuros105006en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4344600731&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume94en_HK
dc.identifier.issue2-3en_HK
dc.identifier.spage295en_HK
dc.identifier.epage300en_HK
dc.identifier.isiWOS:000223877200011-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridKumar, DSS=7402293889en_HK
dc.identifier.scopusauthoridCheung, HY=7201839371en_HK
dc.identifier.scopusauthoridLau, CS=14035682100en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridHyde, KD=7102588111en_HK

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