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Article: Separation and determination of astaxanthin from microalgal and yeast samples by molecularly imprinted microspheres

TitleSeparation and determination of astaxanthin from microalgal and yeast samples by molecularly imprinted microspheres
Authors
KeywordsAstaxanthin
Molecularly imprinted microspheres
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromb
Citation
Journal Of Chromatography B: Analytical Technologies In The Biomedical And Life Sciences, 2004, v. 804 n. 1, p. 25-30 How to Cite?
AbstractIn this work, molecularly imprinted microspheres (MIMs) were synthesized by aqueous microsuspension polymerization using astaxanthin (3,3′- dihydroxy-β,β′-carotene-4,4′-dione) as imprinting molecule. The MIMs obtained were subsequently packed into the stainless steel column and the chromatographic characterization of the column was investigated. The effects of pH and composition of the mobile phase on the retention factor (k′) were investigated in detail. The mixture of methanol and dichloromethane (DCM) (8:2, v/v) was used as mobile phase A while the mixture of methanol and water (5:5, v/v) as mobile phase B. The separation of astaxanthin and zeaxanthin (3,3′-dihydroxyl-β-carotene) was obtained when the concentration of mobile phase B was higher than 30% (v/v) due to their strong lipophilicity. The method developed was successfully applied to separate astaxanthin in the saponified samples of the microalga Haematococcus pluvialis and the yeast Phaffia rhodozyma. The recovery of adding 40mg astaxanthin to 1. 0g microalgal sample was 95.5% with an R.S.D. (n=5) of 5.3%. The results of determination of astaxanthin in the microalga and the yeast were 3.7% (R.S.D.=1.5%, n=9) and 0.041% (R.S.D.=7.3%, n=9), respectively. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68457
ISSN
2015 Impact Factor: 2.687
2015 SCImago Journal Rankings: 0.947
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, JPen_HK
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorHe, XWen_HK
dc.contributor.authorHuang, JCen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:47Z-
dc.date.available2010-09-06T06:04:47Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Chromatography B: Analytical Technologies In The Biomedical And Life Sciences, 2004, v. 804 n. 1, p. 25-30en_HK
dc.identifier.issn1570-0232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68457-
dc.description.abstractIn this work, molecularly imprinted microspheres (MIMs) were synthesized by aqueous microsuspension polymerization using astaxanthin (3,3′- dihydroxy-β,β′-carotene-4,4′-dione) as imprinting molecule. The MIMs obtained were subsequently packed into the stainless steel column and the chromatographic characterization of the column was investigated. The effects of pH and composition of the mobile phase on the retention factor (k′) were investigated in detail. The mixture of methanol and dichloromethane (DCM) (8:2, v/v) was used as mobile phase A while the mixture of methanol and water (5:5, v/v) as mobile phase B. The separation of astaxanthin and zeaxanthin (3,3′-dihydroxyl-β-carotene) was obtained when the concentration of mobile phase B was higher than 30% (v/v) due to their strong lipophilicity. The method developed was successfully applied to separate astaxanthin in the saponified samples of the microalga Haematococcus pluvialis and the yeast Phaffia rhodozyma. The recovery of adding 40mg astaxanthin to 1. 0g microalgal sample was 95.5% with an R.S.D. (n=5) of 5.3%. The results of determination of astaxanthin in the microalga and the yeast were 3.7% (R.S.D.=1.5%, n=9) and 0.041% (R.S.D.=7.3%, n=9), respectively. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromben_HK
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciencesen_HK
dc.rightsJournal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences. Copyright © Elsevier BV.en_HK
dc.subjectAstaxanthinen_HK
dc.subjectMolecularly imprinted microspheresen_HK
dc.titleSeparation and determination of astaxanthin from microalgal and yeast samples by molecularly imprinted microspheresen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1570-0232&volume=804&issue=1&spage=25&epage=30&date=2004&atitle=Separation+and+determination+of+astaxanthin+from+microalgal+and+yeast+samples+by+molecularly+imprinted+microspheresen_HK
dc.identifier.emailHuang, JC: huangjc@hku.hken_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityHuang, JC=rp00710en_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jchromb.2003.12.010en_HK
dc.identifier.pmid15093156-
dc.identifier.scopuseid_2-s2.0-1842715845en_HK
dc.identifier.hkuros90894en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1842715845&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume804en_HK
dc.identifier.issue1en_HK
dc.identifier.spage25en_HK
dc.identifier.epage30en_HK
dc.identifier.isiWOS:000220939100005-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLai, JP=7401939703en_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK
dc.identifier.scopusauthoridHe, XW=7404409073en_HK
dc.identifier.scopusauthoridHuang, JC=7408108735en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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