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Article: Application of statistically-based experimental designs for the optimization of eicosapentaenoic acid production by the diatom Nitzschia laevis

TitleApplication of statistically-based experimental designs for the optimization of eicosapentaenoic acid production by the diatom Nitzschia laevis
Authors
KeywordsDiatom
EPA
Experimental design
Heterotrophic
Nitzschia laevis
Optimization
Issue Date2001
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/71002188
Citation
Biotechnology And Bioengineering, 2001, v. 75 n. 2, p. 159-169 How to Cite?
AbstractStatistically based experimental designs were applied to the optimization of medium components and environmental factors for eicosapentaenoic acid (EPA) production by the diatom Nitzschia laevis in heterotrophic conditions. First, the Plackett-Burman design was used to evaluate the effects of variables including medium components and environmental factors on cell growth and EPA production. Among these variables, NaCl, CaCl2, Pl metal solution, pH, and temperature were identified to have the significant effects (with confidence level > 90 %). Subsequently, the concentrations of NaCl, CaCl2, Pl metal solution as well as the values of pH and temperature were optimized using central composite design. The cell growth and EPA production were found to respectively correlate to NaCl, CaCl2, pH, and temperature that could be represented by second-order polynomial models. The optimal values of the four parameters were determined by response surface and numerical analyses as 8 g/L NaCl, 0.10 g/L CaCl2, pH 8.5 and 19.8°C for cell dry weight (DW), and 14 g/L NaCl, 0.10 g/L CaCl2, pH 8.5 and 18°C for EPA production, respectively. The subsequent verification experiments confirmed the validity of the models. This optimization strategy led to a DW of 9 g/L, an EPA yield of 280 mg/L and an EPA productivity of 28 mg/L/d, respectively, which were considerably higher than those obtained in the previous studies. © 2001 John Wiley & Sons, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/68452
ISSN
2015 Impact Factor: 4.243
2015 SCImago Journal Rankings: 1.633
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWen, ZYen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:44Z-
dc.date.available2010-09-06T06:04:44Z-
dc.date.issued2001en_HK
dc.identifier.citationBiotechnology And Bioengineering, 2001, v. 75 n. 2, p. 159-169en_HK
dc.identifier.issn0006-3592en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68452-
dc.description.abstractStatistically based experimental designs were applied to the optimization of medium components and environmental factors for eicosapentaenoic acid (EPA) production by the diatom Nitzschia laevis in heterotrophic conditions. First, the Plackett-Burman design was used to evaluate the effects of variables including medium components and environmental factors on cell growth and EPA production. Among these variables, NaCl, CaCl2, Pl metal solution, pH, and temperature were identified to have the significant effects (with confidence level > 90 %). Subsequently, the concentrations of NaCl, CaCl2, Pl metal solution as well as the values of pH and temperature were optimized using central composite design. The cell growth and EPA production were found to respectively correlate to NaCl, CaCl2, pH, and temperature that could be represented by second-order polynomial models. The optimal values of the four parameters were determined by response surface and numerical analyses as 8 g/L NaCl, 0.10 g/L CaCl2, pH 8.5 and 19.8°C for cell dry weight (DW), and 14 g/L NaCl, 0.10 g/L CaCl2, pH 8.5 and 18°C for EPA production, respectively. The subsequent verification experiments confirmed the validity of the models. This optimization strategy led to a DW of 9 g/L, an EPA yield of 280 mg/L and an EPA productivity of 28 mg/L/d, respectively, which were considerably higher than those obtained in the previous studies. © 2001 John Wiley & Sons, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/71002188en_HK
dc.relation.ispartofBiotechnology and Bioengineeringen_HK
dc.rightsBiotechnology and Bioengineering. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectDiatomen_HK
dc.subjectEPAen_HK
dc.subjectExperimental designen_HK
dc.subjectHeterotrophicen_HK
dc.subjectNitzschia laevisen_HK
dc.subjectOptimizationen_HK
dc.titleApplication of statistically-based experimental designs for the optimization of eicosapentaenoic acid production by the diatom Nitzschia laevisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3592&volume=75&issue=2&spage=159&epage=169&date=2001&atitle=Application+of+statistically-based+experimental+designs+for+the+optimization+of+eicosapentaenoic+acid+production+by+the+diatom+Nitzschia+laevisen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/bit.1175en_HK
dc.identifier.pmid11536138-
dc.identifier.scopuseid_2-s2.0-0035922856en_HK
dc.identifier.hkuros72644en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035922856&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume75en_HK
dc.identifier.issue2en_HK
dc.identifier.spage159en_HK
dc.identifier.epage169en_HK
dc.identifier.isiWOS:000171118200003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWen, ZY=14012373200en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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