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Article: High-yield production of lutein by the green microalga Chlorella protothecoides in heterotrophic fed-batch culture
Title | High-yield production of lutein by the green microalga Chlorella protothecoides in heterotrophic fed-batch culture |
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Authors | |
Issue Date | 2002 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/bp |
Citation | Biotechnology Progress, 2002, v. 18 n. 4, p. 723-727 How to Cite? |
Abstract | The green microalga Chlorella protothecoides was grown heterotrophically in batch mode in a 3.7-L fermenter containing 40 g/L glucose and 3.6 g/L urea. In the late exponential phase, concentrated nutrients containing glucose and urea were fed into the culture, in which the nitrogen source was sufficient compared to carbon source. As a result, a maximum cell dry weight concentration of 48 g/L was achieved. This cell dry weight concentration was 28.4 g/L higher than that obtained in batch culture under the same growth conditions. In another cultivation run, the culture was provided with the same initial concentrations of glucose (40 g/L) and urea (3.6 g/L) as in the batch mode, followed by a relatively reduced supply of nitrogen source in the fed-batch mode to establish a nitrogen-limited culture. Such a modification resulted in an enhanced lutein production without significantly lowering biomass production. The cellular lutein content was 0.27 mg/g higher than that obtained in the N-sufficient culture. The improvements were also reflected by higher maximum lutein yield, lutein productivity, and lutein yield coefficient on glucose. This N-limited fed-batch culture was successfully scaled up from 3.7 L to 30 L, and a three-step cultivation process was developed for the high-yield production of lutein. The maximum cell dry weight concentration (45.8 g/L) achieved in the large fermenter (30 L) was comparable to that in the small one (3.7 L). The maintenance of the culture at a higher temperature (i.e., 32°C) for 84 h resulted in a 19.9% increase in lutein content but a 13.6% decrease in cell dry weight concentration as compared to the fed-batch culture (30 L) without such a treatment. The enhancement of lutein production resulted from the combination of nitrogen limitation and high-temperature stress. |
Persistent Identifier | http://hdl.handle.net/10722/68449 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.527 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Shi, XM | en_HK |
dc.contributor.author | Jiang, Y | en_HK |
dc.contributor.author | Chen, F | en_HK |
dc.date.accessioned | 2010-09-06T06:04:43Z | - |
dc.date.available | 2010-09-06T06:04:43Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | Biotechnology Progress, 2002, v. 18 n. 4, p. 723-727 | en_HK |
dc.identifier.issn | 8756-7938 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/68449 | - |
dc.description.abstract | The green microalga Chlorella protothecoides was grown heterotrophically in batch mode in a 3.7-L fermenter containing 40 g/L glucose and 3.6 g/L urea. In the late exponential phase, concentrated nutrients containing glucose and urea were fed into the culture, in which the nitrogen source was sufficient compared to carbon source. As a result, a maximum cell dry weight concentration of 48 g/L was achieved. This cell dry weight concentration was 28.4 g/L higher than that obtained in batch culture under the same growth conditions. In another cultivation run, the culture was provided with the same initial concentrations of glucose (40 g/L) and urea (3.6 g/L) as in the batch mode, followed by a relatively reduced supply of nitrogen source in the fed-batch mode to establish a nitrogen-limited culture. Such a modification resulted in an enhanced lutein production without significantly lowering biomass production. The cellular lutein content was 0.27 mg/g higher than that obtained in the N-sufficient culture. The improvements were also reflected by higher maximum lutein yield, lutein productivity, and lutein yield coefficient on glucose. This N-limited fed-batch culture was successfully scaled up from 3.7 L to 30 L, and a three-step cultivation process was developed for the high-yield production of lutein. The maximum cell dry weight concentration (45.8 g/L) achieved in the large fermenter (30 L) was comparable to that in the small one (3.7 L). The maintenance of the culture at a higher temperature (i.e., 32°C) for 84 h resulted in a 19.9% increase in lutein content but a 13.6% decrease in cell dry weight concentration as compared to the fed-batch culture (30 L) without such a treatment. The enhancement of lutein production resulted from the combination of nitrogen limitation and high-temperature stress. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/bp | en_HK |
dc.relation.ispartof | Biotechnology Progress | en_HK |
dc.title | High-yield production of lutein by the green microalga Chlorella protothecoides in heterotrophic fed-batch culture | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0278-9728&volume=18&spage=723&epage=727&date=2002&atitle=High-yield+production+of+lutein+by+the+green+microalga+Chlorella+protothecoides+in+heterotrophic+fed-batch+culture | en_HK |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_HK |
dc.identifier.authority | Chen, F=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/bp0101987 | en_HK |
dc.identifier.scopus | eid_2-s2.0-0036021987 | en_HK |
dc.identifier.hkuros | 80818 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036021987&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 18 | en_HK |
dc.identifier.issue | 4 | en_HK |
dc.identifier.spage | 723 | en_HK |
dc.identifier.epage | 727 | en_HK |
dc.identifier.isi | WOS:000177285200007 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Shi, XM=7402953728 | en_HK |
dc.identifier.scopusauthorid | Jiang, Y=24605346600 | en_HK |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_HK |
dc.identifier.issnl | 1520-6033 | - |