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Article: F-Actin visualization in generative and sperm cells of living pollen of rice using a GFP-Mouse talin fusion protein

TitleF-Actin visualization in generative and sperm cells of living pollen of rice using a GFP-Mouse talin fusion protein
Authors
KeywordsActin microfilament
Generative cell
Green fluorescent protein (GFP)
Living pollen
Oryza sativa
Sperm cells
Issue Date2003
Citation
Acta Botanica Sinica, 2003, v. 45 n. 8, p. 949-958 How to Cite?
AbstractGreen fluorescent protein (GFP) fused to the F-acton binding domain of mouse talin labels the actin cytoskeleton in the living generative and sperm cells of a third generation transgenic rice (Oryza sativa L.) plant, A005-G-T 1-2. Observations were made on pollen at four major developmental stages, viz. I. uni-nucleate microspore stage; II. early bi-cellular pollen stage; III. late bi-cellular pollen stage; and IV. tri-cellular pollen stage. At each of these developmental stages vegetative nucleus, generative nucleus/ cell, and sperm cells were seen undergoing continuous and coordinated motion and migration. These movements seemed to be influenced by associated microfilament networks existing in the pollen. Based on these observations we propose that it is the interaction between the microfilament networks (usually one existing in the central cytoplasm and another in the cortex) that controls the dynamic movement of the vegetative nucleus, generative nucleus/cell and sperm cells. Furthermore, we have also observed that there is an array of microfilaments (oriented mostly parallel to the long axis of the cell) existing in the generative and sperm cells. As far as we are aware, this is the first report showing the existence of microfilaments in living generative and sperm cells of rice pollen. The implication and significance of the existence of microfilaments in generative and sperm cells in rendering self-propelled motion of these cells in relation to their passage and movement in the pollen tube and embryo sac for fertilization were discussed.
Persistent Identifierhttp://hdl.handle.net/10722/68436
ISSN
2003 Impact Factor: 0.321
2007 SCImago Journal Rankings: 0.289
References

 

DC FieldValueLanguage
dc.contributor.authorXu, SXen_HK
dc.contributor.authorYe, XLen_HK
dc.contributor.authorWang, LJen_HK
dc.contributor.authorQiu, ZPen_HK
dc.contributor.authorYe, YJen_HK
dc.date.accessioned2010-09-06T06:04:36Z-
dc.date.available2010-09-06T06:04:36Z-
dc.date.issued2003en_HK
dc.identifier.citationActa Botanica Sinica, 2003, v. 45 n. 8, p. 949-958en_HK
dc.identifier.issn0577-7496en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68436-
dc.description.abstractGreen fluorescent protein (GFP) fused to the F-acton binding domain of mouse talin labels the actin cytoskeleton in the living generative and sperm cells of a third generation transgenic rice (Oryza sativa L.) plant, A005-G-T 1-2. Observations were made on pollen at four major developmental stages, viz. I. uni-nucleate microspore stage; II. early bi-cellular pollen stage; III. late bi-cellular pollen stage; and IV. tri-cellular pollen stage. At each of these developmental stages vegetative nucleus, generative nucleus/ cell, and sperm cells were seen undergoing continuous and coordinated motion and migration. These movements seemed to be influenced by associated microfilament networks existing in the pollen. Based on these observations we propose that it is the interaction between the microfilament networks (usually one existing in the central cytoplasm and another in the cortex) that controls the dynamic movement of the vegetative nucleus, generative nucleus/cell and sperm cells. Furthermore, we have also observed that there is an array of microfilaments (oriented mostly parallel to the long axis of the cell) existing in the generative and sperm cells. As far as we are aware, this is the first report showing the existence of microfilaments in living generative and sperm cells of rice pollen. The implication and significance of the existence of microfilaments in generative and sperm cells in rendering self-propelled motion of these cells in relation to their passage and movement in the pollen tube and embryo sac for fertilization were discussed.en_HK
dc.languageengen_HK
dc.relation.ispartofActa Botanica Sinicaen_HK
dc.subjectActin microfilamenten_HK
dc.subjectGenerative cellen_HK
dc.subjectGreen fluorescent protein (GFP)en_HK
dc.subjectLiving pollenen_HK
dc.subjectOryza sativaen_HK
dc.subjectSperm cellsen_HK
dc.titleF-Actin visualization in generative and sperm cells of living pollen of rice using a GFP-Mouse talin fusion proteinen_HK
dc.typeArticleen_HK
dc.identifier.emailYe, YJ: wkyip@hkucc.hku.hken_HK
dc.identifier.authorityYe, YJ=rp00833en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0141648462en_HK
dc.identifier.hkuros81365en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141648462&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue8en_HK
dc.identifier.spage949en_HK
dc.identifier.epage958en_HK
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridXu, SX=7404438821en_HK
dc.identifier.scopusauthoridYe, XL=55334959900en_HK
dc.identifier.scopusauthoridWang, LJ=7409175686en_HK
dc.identifier.scopusauthoridQiu, ZP=7202941720en_HK
dc.identifier.scopusauthoridYe, YJ=7102784428en_HK

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