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- Publisher Website: 10.1016/j.chroma.2003.09.022
- Scopus: eid_2-s2.0-0344256459
- PMID: 14760860
- WOS: WOS:000187719900017
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Article: Preparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon
Title | Preparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon |
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Authors | |
Keywords | Counter-current chromatography Lithospermum erythrorhizon Pharmaceutical analysis Plant materials Preparative chromatography Shikonin |
Issue Date | 2004 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma |
Citation | Journal Of Chromatography A, 2004, v. 1023 n. 1, p. 159-163 How to Cite? |
Abstract | The bioactive compound shikonin was successfully isolated and purified from the crude extract of the traditional Chinese medicinal plant Lithospermum erythrorhizon Sieb. et Zucc. by preparative high-speed counter-current chromatography (HSCCC). The preparative HSCCC was performed using a two-phase solvent system composed of n-hexane-ethylacetate-ethanol-water (16:14:14:5 (v/v)). A total amount of 19.6mg of shikonin at 98.9% purity was obtained from 52mg of the crude extract (containing 38.9% shikonin) with 96.9% recovery. The preparative isolation and purification of shikonin by HSCCC was completed in 200min in a one-step separation. © 2003 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/68435 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.717 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Lu, HT | en_HK |
dc.contributor.author | Jiang, Y | en_HK |
dc.contributor.author | Chen, F | en_HK |
dc.date.accessioned | 2010-09-06T06:04:35Z | - |
dc.date.available | 2010-09-06T06:04:35Z | - |
dc.date.issued | 2004 | en_HK |
dc.identifier.citation | Journal Of Chromatography A, 2004, v. 1023 n. 1, p. 159-163 | en_HK |
dc.identifier.issn | 0021-9673 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/68435 | - |
dc.description.abstract | The bioactive compound shikonin was successfully isolated and purified from the crude extract of the traditional Chinese medicinal plant Lithospermum erythrorhizon Sieb. et Zucc. by preparative high-speed counter-current chromatography (HSCCC). The preparative HSCCC was performed using a two-phase solvent system composed of n-hexane-ethylacetate-ethanol-water (16:14:14:5 (v/v)). A total amount of 19.6mg of shikonin at 98.9% purity was obtained from 52mg of the crude extract (containing 38.9% shikonin) with 96.9% recovery. The preparative isolation and purification of shikonin by HSCCC was completed in 200min in a one-step separation. © 2003 Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma | en_HK |
dc.relation.ispartof | Journal of Chromatography A | en_HK |
dc.rights | Journal of Chromatography A. Copyright © Elsevier BV. | en_HK |
dc.subject | Counter-current chromatography | en_HK |
dc.subject | Lithospermum erythrorhizon | en_HK |
dc.subject | Pharmaceutical analysis | en_HK |
dc.subject | Plant materials | en_HK |
dc.subject | Preparative chromatography | en_HK |
dc.subject | Shikonin | en_HK |
dc.title | Preparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1023&issue=1&spage=159&epage=163&date=2004&atitle=Preparative+high-speed+counter-current+chromatography+for+purification+of+shikonin+from+the+Chinese+medicinal+plant+Lithospermum+erythrorhizon | en_HK |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_HK |
dc.identifier.authority | Chen, F=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.chroma.2003.09.022 | en_HK |
dc.identifier.pmid | 14760860 | - |
dc.identifier.scopus | eid_2-s2.0-0344256459 | en_HK |
dc.identifier.hkuros | 90923 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0344256459&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 1023 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 159 | en_HK |
dc.identifier.epage | 163 | en_HK |
dc.identifier.isi | WOS:000187719900017 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Lu, HT=7404843255 | en_HK |
dc.identifier.scopusauthorid | Jiang, Y=24605346600 | en_HK |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_HK |
dc.identifier.issnl | 0021-9673 | - |