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Article: Preparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon

TitlePreparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon
Authors
KeywordsCounter-current chromatography
Lithospermum erythrorhizon
Pharmaceutical analysis
Plant materials
Preparative chromatography
Shikonin
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2004, v. 1023 n. 1, p. 159-163 How to Cite?
AbstractThe bioactive compound shikonin was successfully isolated and purified from the crude extract of the traditional Chinese medicinal plant Lithospermum erythrorhizon Sieb. et Zucc. by preparative high-speed counter-current chromatography (HSCCC). The preparative HSCCC was performed using a two-phase solvent system composed of n-hexane-ethylacetate-ethanol-water (16:14:14:5 (v/v)). A total amount of 19.6mg of shikonin at 98.9% purity was obtained from 52mg of the crude extract (containing 38.9% shikonin) with 96.9% recovery. The preparative isolation and purification of shikonin by HSCCC was completed in 200min in a one-step separation. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68435
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, HTen_HK
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:35Z-
dc.date.available2010-09-06T06:04:35Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Chromatography A, 2004, v. 1023 n. 1, p. 159-163en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68435-
dc.description.abstractThe bioactive compound shikonin was successfully isolated and purified from the crude extract of the traditional Chinese medicinal plant Lithospermum erythrorhizon Sieb. et Zucc. by preparative high-speed counter-current chromatography (HSCCC). The preparative HSCCC was performed using a two-phase solvent system composed of n-hexane-ethylacetate-ethanol-water (16:14:14:5 (v/v)). A total amount of 19.6mg of shikonin at 98.9% purity was obtained from 52mg of the crude extract (containing 38.9% shikonin) with 96.9% recovery. The preparative isolation and purification of shikonin by HSCCC was completed in 200min in a one-step separation. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectLithospermum erythrorhizonen_HK
dc.subjectPharmaceutical analysisen_HK
dc.subjectPlant materialsen_HK
dc.subjectPreparative chromatographyen_HK
dc.subjectShikoninen_HK
dc.titlePreparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizonen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1023&issue=1&spage=159&epage=163&date=2004&atitle=Preparative+high-speed+counter-current+chromatography+for+purification+of+shikonin+from+the+Chinese+medicinal+plant+Lithospermum+erythrorhizonen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2003.09.022en_HK
dc.identifier.pmid14760860-
dc.identifier.scopuseid_2-s2.0-0344256459en_HK
dc.identifier.hkuros90923en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344256459&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1023en_HK
dc.identifier.issue1en_HK
dc.identifier.spage159en_HK
dc.identifier.epage163en_HK
dc.identifier.isiWOS:000187719900017-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLu, HT=7404843255en_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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