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Article: Identification of astaxanthin isomers in Haematococcus lacustris by HPLC-photodiode array detection

TitleIdentification of astaxanthin isomers in Haematococcus lacustris by HPLC-photodiode array detection
Authors
Issue Date1997
PublisherKluwer Academic Publishers. The Journal's web site is located at http://www.wkap.nl
Citation
Biotechnology Techniques, 1997, v. 11 n. 7, p. 455-459 How to Cite?
AbstractAn HPLC method was developed for the separation and identification of the isomers of astaxanthin from the saponification products of the individual astaxanthin ester fractions and the total pigment extract from Haematococcus lacustris. Six astaxanthin ester fractions were initially separated and collected by HPLC. These astaxanthin ester fractions and the total pigment extract were subsequently respectively hydrolysed. Four isomers of astaxanthin in the saponified mixtures were separated and identified respectively as (3S, 3'S)-trans-astaxanthin (478.8 nm), (3S, 3'S)-9-cis-astaxanthin (470.4 nm), (3S, 3's)13-cis-astaxanthin (371.8 and 468.0 nm) and (3R, 3'R)-trans-astaxanthin (477.6 nm) according to their absorbance spectra and absorption maxima by photodiode array detection. The relative contents of these isomers were determined to be 72.8, 9.7, 8.9 and 8.6%, respectively, in Haematococcus lacustris.
Persistent Identifierhttp://hdl.handle.net/10722/68431
ISSN
2001 Impact Factor: 0.697
2002 SCImago Journal Rankings: 0.256
References

 

DC FieldValueLanguage
dc.contributor.authorYuan, JPen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:33Z-
dc.date.available2010-09-06T06:04:33Z-
dc.date.issued1997en_HK
dc.identifier.citationBiotechnology Techniques, 1997, v. 11 n. 7, p. 455-459en_HK
dc.identifier.issn0951-208Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68431-
dc.description.abstractAn HPLC method was developed for the separation and identification of the isomers of astaxanthin from the saponification products of the individual astaxanthin ester fractions and the total pigment extract from Haematococcus lacustris. Six astaxanthin ester fractions were initially separated and collected by HPLC. These astaxanthin ester fractions and the total pigment extract were subsequently respectively hydrolysed. Four isomers of astaxanthin in the saponified mixtures were separated and identified respectively as (3S, 3'S)-trans-astaxanthin (478.8 nm), (3S, 3'S)-9-cis-astaxanthin (470.4 nm), (3S, 3's)13-cis-astaxanthin (371.8 and 468.0 nm) and (3R, 3'R)-trans-astaxanthin (477.6 nm) according to their absorbance spectra and absorption maxima by photodiode array detection. The relative contents of these isomers were determined to be 72.8, 9.7, 8.9 and 8.6%, respectively, in Haematococcus lacustris.en_HK
dc.languageengen_HK
dc.publisherKluwer Academic Publishers. The Journal's web site is located at http://www.wkap.nlen_HK
dc.relation.ispartofBiotechnology Techniquesen_HK
dc.rightsBiotechnology Techniques. Copyright © Kluwer Academic Publishers.en_HK
dc.titleIdentification of astaxanthin isomers in Haematococcus lacustris by HPLC-photodiode array detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0951-208X&volume=11&issue=7&spage=455&epage=459&date=1997&atitle=Identification+of+astaxanthin+isomers+in+Haematococcus+lacustris+by+HPLC-photodiode+array+detectionen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0030758576en_HK
dc.identifier.hkuros35165en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030758576&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue7en_HK
dc.identifier.spage455en_HK
dc.identifier.epage459en_HK
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridYuan, JP=7403401467en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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