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Article: Chromatographic Separation and Purification of trans-Astaxanthin from the Extracts of Haematococcus pluvialis

TitleChromatographic Separation and Purification of trans-Astaxanthin from the Extracts of Haematococcus pluvialis
Authors
KeywordsAstaxanthin
Astaxanthin esters
Carotenoids
Haematococcus pluvialis
HPLC
Purification
Issue Date1998
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 1998, v. 46 n. 8, p. 3371-3375 How to Cite?
AbstractA gradient reversed-phase HPLC method was developed for the separation of astaxanthin esters and the isomers of astaxanthin from the unsaponified and saponified pigment extracts of the microalga Haematococcus pluvialis. Four kinds of isomers of astaxanthin and astaxanthin esters, (3S,3′S)-trans-astaxanthin, (3S,3′S)-9-cis-astaxanthin, (3S,3′S)-13-cis-astaxanthin, (3R,3′R)-trans-astaxanthin, and their esters, were separated and identified. A small amount of (3S,3′S)-15-trans-astaxanthin was also detected from the saponified extract. In addition, a chromatographic purification method was established for the preparation of natural trans-astaxanthin from the saponified extract of H. pluvialis. With this method, 3.7 mg of astaxanthin was isolated from 1 g of dry biomass of H. pluvialis. The purified astaxanthin contained approximately 97.7% trans-astaxanthin, 1.1% cis-astaxanthin, and 1.2% impurity.
Persistent Identifierhttp://hdl.handle.net/10722/68407
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
References

 

DC FieldValueLanguage
dc.contributor.authorYuan, JPen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:21Z-
dc.date.available2010-09-06T06:04:21Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 1998, v. 46 n. 8, p. 3371-3375en_HK
dc.identifier.issn0021-8561en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68407-
dc.description.abstractA gradient reversed-phase HPLC method was developed for the separation of astaxanthin esters and the isomers of astaxanthin from the unsaponified and saponified pigment extracts of the microalga Haematococcus pluvialis. Four kinds of isomers of astaxanthin and astaxanthin esters, (3S,3′S)-trans-astaxanthin, (3S,3′S)-9-cis-astaxanthin, (3S,3′S)-13-cis-astaxanthin, (3R,3′R)-trans-astaxanthin, and their esters, were separated and identified. A small amount of (3S,3′S)-15-trans-astaxanthin was also detected from the saponified extract. In addition, a chromatographic purification method was established for the preparation of natural trans-astaxanthin from the saponified extract of H. pluvialis. With this method, 3.7 mg of astaxanthin was isolated from 1 g of dry biomass of H. pluvialis. The purified astaxanthin contained approximately 97.7% trans-astaxanthin, 1.1% cis-astaxanthin, and 1.2% impurity.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_HK
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_HK
dc.subjectAstaxanthinen_HK
dc.subjectAstaxanthin estersen_HK
dc.subjectCarotenoidsen_HK
dc.subjectHaematococcus pluvialisen_HK
dc.subjectHPLCen_HK
dc.subjectPurificationen_HK
dc.titleChromatographic Separation and Purification of trans-Astaxanthin from the Extracts of Haematococcus pluvialisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8561&volume=46&issue=8&spage=3371&epage=3375&date=1998&atitle=Chromatographic+separation+and+purification+of+trans-astaxanthin+from+the+extracts+of+Haematococcus+pluvialisen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0001599028en_HK
dc.identifier.hkuros45459en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0001599028&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue8en_HK
dc.identifier.spage3371en_HK
dc.identifier.epage3375en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYuan, JP=7403401467en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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